Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACK3BE_RS27030 Genome accession   NZ_CP177237
Coordinates   5908814..5910031 (+) Length   405 a.a.
NCBI ID   WP_010467583.1    Uniprot ID   A0A024EER5
Organism   Pseudomonas mandelii strain VR4' HT30-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5903814..5915031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3BE_RS27010 (ACK3BE_27010) - 5905232..5905528 (+) 297 WP_282411970.1 DUF2845 domain-containing protein -
  ACK3BE_RS27015 (ACK3BE_27015) - 5905812..5906165 (-) 354 WP_010467586.1 BON domain-containing protein -
  ACK3BE_RS27020 (ACK3BE_27020) pilA 5906478..5906885 (-) 408 WP_010467585.1 pilin Machinery gene
  ACK3BE_RS27025 (ACK3BE_27025) pilB 5907111..5908811 (+) 1701 WP_083375734.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK3BE_RS27030 (ACK3BE_27030) pilC 5908814..5910031 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  ACK3BE_RS27035 (ACK3BE_27035) pilD 5910033..5910905 (+) 873 WP_311096686.1 A24 family peptidase Machinery gene
  ACK3BE_RS27040 (ACK3BE_27040) coaE 5910902..5911525 (+) 624 WP_102593899.1 dephospho-CoA kinase -
  ACK3BE_RS27045 (ACK3BE_27045) yacG 5911522..5911722 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  ACK3BE_RS27050 (ACK3BE_27050) - 5911726..5911941 (-) 216 WP_010467577.1 hypothetical protein -
  ACK3BE_RS27055 (ACK3BE_27055) - 5912005..5912694 (-) 690 WP_083375730.1 energy-coupling factor ABC transporter permease -
  ACK3BE_RS27060 (ACK3BE_27060) - 5912691..5913158 (-) 468 WP_010467572.1 hypothetical protein -
  ACK3BE_RS27065 (ACK3BE_27065) - 5913288..5913914 (+) 627 WP_007970534.1 DUF1780 domain-containing protein -
  ACK3BE_RS27070 (ACK3BE_27070) - 5913911..5914438 (+) 528 WP_010467568.1 MOSC domain-containing protein -
  ACK3BE_RS27075 (ACK3BE_27075) - 5914490..5914663 (+) 174 WP_008028730.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44190.99 Da        Isoelectric Point: 9.7373

>NTDB_id=1086339 ACK3BE_RS27030 WP_010467583.1 5908814..5910031(+) (pilC) [Pseudomonas mandelii strain VR4' HT30-3]
MAVKAAKISVYAWEGTDRKGSKMTGELSGLNPALIKAQLRKQGINPGKVRKKSASIFSFGNRIKAQEIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLIKVVPQFESVFKGFGAELPAFTVMVIGLSEFLQAWWWMMLGALIAAIFGVR
HAFKTSEAFRDRTDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPSMAIQLTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1086339 ACK3BE_RS27030 WP_010467583.1 5908814..5910031(+) (pilC) [Pseudomonas mandelii strain VR4' HT30-3]
ATGGCGGTCAAAGCAGCAAAAATCAGCGTCTACGCCTGGGAAGGCACGGACAGGAAAGGATCAAAGATGACCGGCGAGTT
GAGCGGTCTCAACCCGGCCTTGATCAAGGCTCAGTTGCGCAAGCAGGGGATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CCTCGATTTTCAGTTTCGGCAATCGGATCAAGGCTCAGGAAATCGCCCTGTTTACCCGGCAAATGGCAACGATGATGAAA
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATCGGTGAAGGCTTCGACAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAGCAGGAAGTCGCGGCGGGTAACAGCTTCGCTGCGTCCTTGCGTAAAAAACCGCAGTATTTCGACGAGCTGTACT
GCAACCTCGTCGACGCAGGTGAGCAGGCTGGCGCTCTGGATACCCTGCTGGAACGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCCGTGGTCTTTGTGGCGATCATTGTCACCGGGAT
TCTGCTGATCAAGGTGGTGCCGCAGTTCGAGTCGGTGTTCAAGGGCTTTGGCGCCGAGTTGCCGGCCTTCACCGTGATGG
TCATCGGCTTGTCGGAGTTCTTGCAGGCCTGGTGGTGGATGATGCTTGGCGCGTTGATCGCCGCGATCTTCGGTGTGCGC
CACGCCTTTAAAACATCCGAGGCCTTTCGGGACCGCACGGACACCTGGCTGCTGAAGCTGCCGCTGATCGGCACCTTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCCTGTCGACCACATTCGCCGCCGGCGTGCCGTTGGTCGAAGCCCTGG
ATTCAGTGGCGGGCGCGACTGGCAACATCGTGTTCAAACGGGCGGTGCTGCGCATCAAGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTCTCGATGCGCACCTCCGGCATCTTTCCCAGTATGGCGATCCAGCTGACCGCCATTGGCGAAGAATCCGG
CGCTCTGGACGACATGCTCGATAAGGTCGCGAGCTTCTACGAGGACGAAGTGGACAATATGGTCGATAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATTGTCGGTGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EER5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

58.333

100

0.588
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

40.148

100

0.402
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment