Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLB90_RS12425 Genome accession   NZ_CP177163
Coordinates   2890541..2891671 (-) Length   376 a.a.
NCBI ID   WP_411849790.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LGBM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2885541..2896671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS12410 (ACLB90_12410) - 2887035..2888420 (-) 1386 WP_411849787.1 DEAD/DEAH box helicase -
  ACLB90_RS12415 (ACLB90_12415) - 2888837..2889826 (+) 990 WP_411849788.1 fumarylacetoacetate hydrolase family protein -
  ACLB90_RS12420 (ACLB90_12420) maiA 2889847..2890503 (+) 657 WP_411849789.1 maleylacetoacetate isomerase -
  ACLB90_RS12425 (ACLB90_12425) pilU 2890541..2891671 (-) 1131 WP_411849790.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLB90_RS12430 (ACLB90_12430) - 2891771..2892157 (+) 387 WP_411849791.1 DUF4398 domain-containing protein -
  ACLB90_RS12435 (ACLB90_12435) - 2892169..2893038 (+) 870 WP_411852754.1 hypothetical protein -
  ACLB90_RS12440 (ACLB90_12440) - 2893383..2893601 (+) 219 WP_411849792.1 YdcH family protein -
  ACLB90_RS12445 (ACLB90_12445) - 2893836..2895206 (+) 1371 WP_411849793.1 pyridoxal-phosphate dependent enzyme -
  ACLB90_RS12450 (ACLB90_12450) - 2895334..2896518 (+) 1185 WP_411849794.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41460.79 Da        Isoelectric Point: 6.3738

>NTDB_id=1085711 ACLB90_RS12425 WP_411849790.1 2890541..2891671(-) (pilU) [Stenotrophomonas sp. LGBM10]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNTTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVVSQRLVKGKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMAKSGIIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYSAASPAAITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1085711 ACLB90_RS12425 WP_411849790.1 2890541..2891671(-) (pilU) [Stenotrophomonas sp. LGBM10]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGAGCCCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTACCCGCTGGGGAACACCGGCCTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCGGACGCGGGACGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCCATCCGCAGCAAGATCCCGAGCATCGA
AGAGCTCAATCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAATCGACCTCACTGGCCTCGATGATCGATCACCGCAACACCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGTGCGCACCGCAACGTGCTGATGAACCTCTCGCTGAACCTGCGCGCGGTGGTCTCCCAGCG
CCTGGTCAAGGGCAAGGACGGACGCCGCCTGCCGGCCACCGAGGTACTGATCAACACCCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCCGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGATGGCCAAGTCCGGCATCATCGAGCAGGAAGAGGCGCTGCGCGCGGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAACACGACCCCTACGCGGATTATTCGGCCGCGAGCCCGGCGGCCATCACCC
ACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

95.479

0.495
  pilU Acinetobacter baylyi ADP1

49.718

94.149

0.468
  pilU Vibrio cholerae strain A1552

48.611

95.745

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.449
  pilT Pseudomonas aeruginosa PAK

42.899

89.894

0.386
  pilT Acinetobacter baylyi ADP1

41.471

90.426

0.375
  pilT Acinetobacter baumannii D1279779

41.716

89.894

0.375
  pilT Acinetobacter baumannii strain A118

41.716

89.894

0.375
  pilT Acinetobacter nosocomialis M2

41.716

89.894

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.42

89.894

0.372
  pilT Legionella pneumophila strain Lp02

40.828

89.894

0.367
  pilT Legionella pneumophila strain ERS1305867

40.828

89.894

0.367
  pilT Neisseria gonorrhoeae MS11

39.193

92.287

0.362
  pilT Neisseria meningitidis 8013

39.193

92.287

0.362

Multiple sequence alignment