Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACK1U3_RS08150 Genome accession   NZ_CP176537
Coordinates   1811811..1812809 (-) Length   332 a.a.
NCBI ID   WP_409488950.1    Uniprot ID   -
Organism   Pseudomonas promysalinigenes strain KZH.chiA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1806811..1817809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK1U3_RS08130 (ACK1U3_08130) argS 1808029..1809765 (+) 1737 WP_409488949.1 arginine--tRNA ligase -
  ACK1U3_RS08135 (ACK1U3_08135) - 1809767..1810468 (+) 702 WP_207937374.1 SPOR domain-containing protein -
  ACK1U3_RS08140 (ACK1U3_08140) - 1810521..1810940 (-) 420 WP_060479878.1 NINE protein -
  ACK1U3_RS08145 (ACK1U3_08145) - 1811107..1811736 (+) 630 WP_186476262.1 C40 family peptidase -
  ACK1U3_RS08150 (ACK1U3_08150) pilT 1811811..1812809 (-) 999 WP_409488950.1 type IV pilus twitching motility protein PilT Machinery gene
  ACK1U3_RS08155 (ACK1U3_08155) - 1812876..1813562 (+) 687 WP_060479875.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACK1U3_RS08160 (ACK1U3_08160) proC 1813601..1814419 (+) 819 WP_186476260.1 pyrroline-5-carboxylate reductase -
  ACK1U3_RS08165 (ACK1U3_08165) - 1814430..1815020 (+) 591 WP_060479873.1 YggT family protein -
  ACK1U3_RS08170 (ACK1U3_08170) - 1815119..1816258 (+) 1140 WP_060479872.1 homoserine O-acetyltransferase -
  ACK1U3_RS08175 (ACK1U3_08175) metW 1816266..1816886 (+) 621 WP_186476258.1 methionine biosynthesis protein MetW -
  ACK1U3_RS08180 (ACK1U3_08180) - 1816909..1817346 (+) 438 WP_060479870.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36128.78 Da        Isoelectric Point: 6.8331

>NTDB_id=1080829 ACK1U3_RS08150 WP_409488950.1 1811811..1812809(-) (pilT) [Pseudomonas promysalinigenes strain KZH.chiA]
MDVTDLLARAMDARASDLHLATGEVPMLRVDGALRRMPLPEITAAALGDAITPLLDQSQCRQWQHGDELDLALQLPGLGR
FRLNLFRQFNGLAATIRLIAQRIACVDELDLEEVYQAVARYSDGLVLVGGPTGSGKSSTLAALVDRLNRERALHIITLED
PIEVIHTSQCSLINQREVGRHCRDFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSIDRL
VEVFEAPEKPLVRSMLAESLRLVVAQALVKRVGGGRVAAREVLAATPAVRNLVREGRMAQLCSVMQAGAAEGMRTMEGAI
RGLRERGLISDP

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1080829 ACK1U3_RS08150 WP_409488950.1 1811811..1812809(-) (pilT) [Pseudomonas promysalinigenes strain KZH.chiA]
ATGGATGTGACCGACCTGTTGGCCCGGGCCATGGATGCAAGGGCTAGCGACCTGCACCTGGCAACGGGTGAGGTACCGAT
GCTGCGCGTGGATGGGGCGTTACGGCGCATGCCGCTGCCAGAGATTACGGCAGCCGCGTTGGGCGATGCGATAACGCCCT
TGCTCGATCAGAGCCAGTGCCGGCAATGGCAGCATGGCGATGAGCTGGACCTGGCCTTGCAGTTACCTGGCCTGGGGCGA
TTTCGTCTCAATCTGTTTCGGCAGTTCAATGGTCTGGCCGCCACCATCAGGCTGATTGCTCAGCGCATAGCCTGCGTGGA
TGAGCTCGACCTCGAGGAAGTGTATCAAGCTGTTGCGCGATATTCGGATGGGCTTGTGCTGGTAGGCGGCCCGACCGGCA
GCGGCAAGTCAAGCACCCTGGCGGCCTTGGTCGACCGGCTGAACCGGGAACGGGCGCTGCACATCATCACCCTCGAAGAC
CCCATCGAAGTTATCCACACCAGCCAATGCAGCCTGATCAACCAGCGTGAAGTAGGCCGGCACTGCCGCGACTTCGCGCA
AGGCTTGCGCAGTGCGCTACGTCAGGACCCCGATGTGATCATGATTGGCGAGCTGCGCGATCTGGAAACCATCCGCTTGG
CGTTGCGTGCCGCAGAGACCGGCCATCTGGTGCTGGCCACGGTGCATACGCGGTCTGCTGCAGGCAGCATCGATCGGTTG
GTCGAGGTTTTCGAGGCGCCGGAAAAGCCGCTGGTAAGGTCCATGCTGGCAGAGTCATTACGCTTGGTGGTGGCGCAAGC
GCTGGTCAAGCGGGTGGGCGGTGGCCGTGTCGCGGCGCGTGAGGTGCTGGCGGCGACACCTGCTGTGCGCAATCTGGTGC
GCGAGGGGCGAATGGCGCAGTTATGTTCGGTGATGCAGGCCGGGGCGGCGGAGGGCATGCGGACGATGGAGGGAGCGATT
CGGGGGCTGAGGGAGCGGGGGTTGATTAGTGACCCGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.212

99.398

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

99.096

0.506
  pilT Vibrio cholerae strain A1552

51.064

99.096

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Neisseria meningitidis 8013

50.453

99.699

0.503
  pilT Acinetobacter baylyi ADP1

50.301

100

0.503
  pilT Neisseria gonorrhoeae MS11

50.151

99.699

0.5
  pilT Acinetobacter baumannii strain A118

49.697

99.398

0.494
  pilT Acinetobacter nosocomialis M2

49.697

99.398

0.494
  pilT Acinetobacter baumannii D1279779

49.697

99.398

0.494
  pilT Legionella pneumophila strain Lp02

48.485

99.398

0.482
  pilT Legionella pneumophila strain ERS1305867

48.485

99.398

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.015

99.699

0.449
  pilU Pseudomonas stutzeri DSM 10701

36.716

100

0.37

Multiple sequence alignment