Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFGDV_RS18765 Genome accession   NZ_CP176528
Coordinates   4054461..4055642 (-) Length   393 a.a.
NCBI ID   WP_136414654.1    Uniprot ID   -
Organism   Noviherbaspirillum sp. ST 5-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4049461..4060642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFGDV_RS18735 - 4049996..4050676 (+) 681 WP_136414668.1 dienelactone hydrolase family protein -
  ACFGDV_RS18740 - 4050666..4051484 (+) 819 WP_136414665.1 phosphoribosyltransferase -
  ACFGDV_RS18745 - 4051488..4052090 (+) 603 WP_136414663.1 transglutaminase-like cysteine peptidase -
  ACFGDV_RS18750 - 4052152..4052682 (-) 531 WP_136414661.1 c-type cytochrome -
  ACFGDV_RS18755 - 4052747..4053430 (-) 684 WP_136414659.1 DUF6639 family protein -
  ACFGDV_RS18760 - 4053881..4054246 (+) 366 WP_136414657.1 glutaredoxin domain-containing protein -
  ACFGDV_RS18765 pilU 4054461..4055642 (-) 1182 WP_136414654.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFGDV_RS18770 - 4055698..4056057 (+) 360 WP_136414652.1 hypothetical protein -
  ACFGDV_RS18775 - 4056116..4056529 (+) 414 WP_136414649.1 YqjD family protein -
  ACFGDV_RS18780 - 4056645..4057013 (+) 369 WP_136414647.1 phage holin family protein -
  ACFGDV_RS18785 - 4057010..4057438 (+) 429 WP_136414644.1 hypothetical protein -
  ACFGDV_RS18790 - 4057520..4057657 (+) 138 WP_136414642.1 entericidin A/B family lipoprotein -
  ACFGDV_RS18795 - 4057718..4058905 (-) 1188 WP_136414640.1 AI-2E family transporter -
  ACFGDV_RS18800 - 4058920..4059741 (-) 822 WP_136414638.1 hypothetical protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43825.01 Da        Isoelectric Point: 6.8245

>NTDB_id=1080725 ACFGDV_RS18765 WP_136414654.1 4054461..4055642(-) (pilU) [Noviherbaspirillum sp. ST 5-3]
MSSNFGPDEARAYMHQLLKVMFQQGGSDLFISADFPPSMKHQGAMKALSQQKLTGDITKKLALALMNDKQRQEFETELEC
NFAISLPNVCRFRVNVFVQQQQTGMVIRTIAAEIPSFEKLQLPDTLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNA
NSSGHIITVEDPVEYVHKNKSCLITHREVGVDTLSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTIDRIINFFPEERRNQLLGDLSSNLRAIVSQRLVRTEDGKGRRAAIEILLNTPTIAEQILKGQFHAIKETMAKS
RELGMRTFDQALFDLYNAGHISYDEAIRNADSANELRLQIKLRGERGRQGGAETGGLNLAIDEEKVEEVEEKK

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=1080725 ACFGDV_RS18765 WP_136414654.1 4054461..4055642(-) (pilU) [Noviherbaspirillum sp. ST 5-3]
ATGTCTTCCAACTTCGGCCCCGATGAAGCGCGCGCCTACATGCATCAACTTCTGAAGGTGATGTTTCAGCAGGGCGGCTC
GGATCTGTTCATCTCCGCGGACTTCCCGCCCAGCATGAAGCACCAGGGCGCGATGAAGGCGCTCAGCCAGCAGAAGCTGA
CCGGCGACATCACGAAAAAGCTGGCGCTGGCACTGATGAACGACAAGCAGCGGCAGGAATTCGAAACCGAGCTGGAATGC
AATTTCGCGATTTCGCTGCCCAACGTGTGCCGCTTCCGCGTCAACGTCTTTGTGCAGCAACAGCAGACCGGCATGGTGAT
CCGCACCATCGCCGCCGAAATCCCCAGCTTCGAAAAACTGCAGTTGCCCGACACGCTCAAGGACGTGATCATGACCAAGC
GCGGACTCGTGCTGGTCGTCGGCGGCACCGGCTCCGGCAAATCGACCACGCTGGCGGCGATGATCGATCACCGCAACGCC
AACTCCTCCGGTCACATCATCACCGTCGAAGATCCGGTCGAATATGTGCACAAGAACAAGTCCTGCCTGATCACGCATCG
CGAGGTCGGCGTCGATACGCTCTCATGGCATCACGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCG
GCGAGATCCGCGATACGGAAACCATGGAGCATGCAATCGCCTTCGCCGAAACCGGCCACCTCTGCCTCGGTACGCTGCAC
GCGAACAACGCCAACCAGACCATCGACCGCATCATCAACTTCTTCCCGGAAGAGAGGCGCAACCAGTTGCTGGGCGACCT
CTCGTCCAACCTGCGCGCCATCGTTTCGCAACGCCTGGTGCGCACCGAAGACGGCAAGGGCCGACGCGCCGCAATCGAAA
TCCTGCTCAACACGCCGACCATCGCCGAACAGATTCTCAAAGGCCAGTTCCACGCGATCAAGGAAACCATGGCCAAATCC
AGGGAACTCGGCATGCGCACCTTCGACCAGGCCTTGTTCGACCTGTACAACGCGGGGCATATCAGTTACGACGAAGCCAT
CCGCAATGCGGATTCGGCGAATGAGCTGCGCCTGCAGATCAAGCTGCGCGGCGAGCGCGGACGGCAGGGCGGCGCCGAAA
CGGGCGGCCTGAATCTGGCGATCGACGAGGAGAAGGTCGAGGAGGTAGAGGAGAAGAAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.692

92.621

0.534
  pilU Acinetobacter baylyi ADP1

55.807

89.822

0.501
  pilU Vibrio cholerae strain A1552

50.538

94.656

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.529

86.514

0.377
  pilT Acinetobacter baylyi ADP1

44.109

84.224

0.372
  pilT Legionella pneumophila strain Lp02

44.343

83.206

0.369
  pilT Legionella pneumophila strain ERS1305867

44.343

83.206

0.369
  pilT Acinetobacter nosocomialis M2

44.037

83.206

0.366
  pilT Acinetobacter baumannii D1279779

44.037

83.206

0.366
  pilT Acinetobacter baumannii strain A118

44.037

83.206

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.969

80.916

0.364
  pilT Vibrio cholerae strain A1552

44.969

80.916

0.364
  pilT Pseudomonas aeruginosa PAK

43.202

84.224

0.364
  pilT Pseudomonas stutzeri DSM 10701

42.388

85.242

0.361

Multiple sequence alignment