Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ77P_RS10085 Genome accession   NZ_CP175563
Coordinates   2204424..2205506 (-) Length   360 a.a.
NCBI ID   WP_367360236.1    Uniprot ID   -
Organism   Syntrophus buswellii strain DM-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2199424..2210506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ77P_RS10055 (ACJ77P_10055) waaC 2199801..2200874 (+) 1074 WP_367360230.1 lipopolysaccharide heptosyltransferase I -
  ACJ77P_RS10060 (ACJ77P_10060) - 2200947..2201153 (+) 207 WP_367360231.1 Trm112 family protein -
  ACJ77P_RS10065 (ACJ77P_10065) - 2201253..2201564 (+) 312 WP_367360232.1 IscA/HesB family protein -
  ACJ77P_RS10070 (ACJ77P_10070) - 2201649..2202617 (+) 969 WP_367360233.1 lipopolysaccharide assembly protein LapB -
  ACJ77P_RS10075 (ACJ77P_10075) - 2202629..2203141 (-) 513 WP_367360234.1 hypothetical protein -
  ACJ77P_RS10080 (ACJ77P_10080) - 2203158..2204330 (-) 1173 WP_367360235.1 type IV pilus twitching motility protein PilT -
  ACJ77P_RS10085 (ACJ77P_10085) pilT 2204424..2205506 (-) 1083 WP_367360236.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ77P_RS10095 (ACJ77P_10095) - 2206141..2206959 (+) 819 WP_367360237.1 hypothetical protein -
  ACJ77P_RS10100 (ACJ77P_10100) - 2207227..2209215 (+) 1989 WP_367360315.1 alpha-amylase family glycosyl hydrolase -
  ACJ77P_RS10105 (ACJ77P_10105) - 2209370..2209930 (+) 561 WP_408998142.1 hypothetical protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39906.93 Da        Isoelectric Point: 6.4188

>NTDB_id=1078232 ACJ77P_RS10085 WP_367360236.1 2204424..2205506(-) (pilT) [Syntrophus buswellii strain DM-2]
MAKIDAFFQLMHEQGASDLHLISGQQPAIRLRGELERVKYNTLDNDELKAMLYEITPEHKVKQFEETGDVDFAYEIPGLA
RYRANFFEQKFGVGAVFREIPSEILTCEQLGLPPVISKLASLPRGLVLVTGPTGSGKSTTLAAIVNEANRIRKDHIITVE
DPIEFVHQSQSCIVNHREVGIHTQSFSAALRGALREDPDIILVGEMRDLETISLAVEAASTGHLVFGTLHTTSAAKTVDR
VIEVFPSSEQAQIRSTLADGLRAIIAQVLFKRIDKKGRCAALEILIANAAVRNLIRESKTFQIPSMIQTGKKYGMQLLDD
AIMELFNKGWISADEAYMKCNDKAKFRALLKSPPADFTEV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=1078232 ACJ77P_RS10085 WP_367360236.1 2204424..2205506(-) (pilT) [Syntrophus buswellii strain DM-2]
ATGGCAAAAATCGATGCTTTTTTTCAATTGATGCATGAACAGGGCGCATCGGACCTTCACCTCATTTCCGGGCAGCAGCC
TGCCATTCGGCTACGTGGCGAACTGGAGCGGGTCAAGTATAACACCCTGGATAATGATGAACTGAAGGCCATGCTCTATG
AAATCACCCCGGAACACAAGGTCAAGCAGTTCGAGGAAACGGGCGATGTGGATTTTGCCTATGAAATTCCTGGATTGGCC
CGCTACCGTGCCAATTTTTTCGAGCAGAAGTTCGGCGTGGGGGCTGTGTTCCGGGAGATTCCCAGCGAGATCCTGACCTG
CGAGCAGCTGGGACTTCCACCTGTCATTTCCAAGCTGGCCTCGCTCCCCCGGGGGCTGGTCCTCGTGACCGGACCCACCG
GCAGCGGAAAGTCGACGACCCTGGCGGCCATCGTCAATGAAGCAAACCGCATCCGCAAGGATCACATCATCACGGTCGAA
GATCCTATCGAATTTGTCCATCAGAGCCAGAGCTGCATCGTCAATCACCGGGAAGTGGGCATCCACACGCAGAGTTTTTC
GGCGGCTCTCAGAGGCGCCCTCCGTGAAGACCCGGACATCATCCTGGTGGGCGAAATGCGGGATCTGGAAACCATCTCGC
TGGCCGTGGAGGCCGCCTCCACAGGCCACCTCGTCTTCGGGACACTCCACACCACAAGCGCGGCCAAGACGGTTGACCGG
GTCATCGAGGTGTTTCCCTCGAGCGAACAGGCCCAGATCCGCTCCACCCTGGCAGATGGTCTGCGGGCCATCATTGCCCA
GGTTCTGTTCAAAAGAATCGACAAGAAAGGGCGCTGCGCCGCCCTGGAAATCCTTATTGCCAATGCGGCGGTACGCAACC
TGATCCGCGAGTCCAAGACGTTCCAGATCCCCTCCATGATTCAGACGGGGAAAAAGTACGGGATGCAGCTTCTGGATGAC
GCCATCATGGAGCTGTTCAATAAGGGCTGGATCAGCGCCGACGAGGCCTACATGAAGTGCAACGACAAGGCAAAATTCCG
GGCTCTGTTGAAAAGCCCGCCGGCTGATTTCACGGAAGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

56.287

92.778

0.522
  pilT Pseudomonas aeruginosa PAK

55.689

92.778

0.517
  pilT Legionella pneumophila strain Lp02

55.589

91.944

0.511
  pilT Legionella pneumophila strain ERS1305867

55.589

91.944

0.511
  pilT Acinetobacter baylyi ADP1

53.846

93.889

0.506
  pilT Acinetobacter baumannii D1279779

53.593

92.778

0.497
  pilT Acinetobacter baumannii strain A118

53.593

92.778

0.497
  pilT Acinetobacter nosocomialis M2

53.593

92.778

0.497
  pilT Neisseria meningitidis 8013

52.905

90.833

0.481
  pilT Neisseria gonorrhoeae MS11

52.599

90.833

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

90.833

0.472
  pilT Vibrio cholerae strain A1552

51.988

90.833

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.11

96.111

0.453
  pilU Pseudomonas stutzeri DSM 10701

38.395

96.944

0.372

Multiple sequence alignment