Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJJWD_RS23350 Genome accession   NZ_CP174505
Coordinates   5147336..5148472 (+) Length   378 a.a.
NCBI ID   WP_003050582.1    Uniprot ID   B7WR82
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5142336..5153472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS23330 (ACJJWD_23350) - 5143168..5143938 (+) 771 WP_407714452.1 TetR/AcrR family transcriptional regulator -
  ACJJWD_RS23335 (ACJJWD_23355) - 5143986..5145233 (+) 1248 WP_407714453.1 branched-chain amino acid ABC transporter substrate-binding protein -
  ACJJWD_RS23340 (ACJJWD_23360) - 5145388..5146110 (-) 723 WP_211457296.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJJWD_RS23345 (ACJJWD_23365) pilT 5146148..5147191 (+) 1044 WP_003050584.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJJWD_RS23350 (ACJJWD_23370) pilU 5147336..5148472 (+) 1137 WP_003050582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJJWD_RS23355 (ACJJWD_23375) - 5148620..5149531 (+) 912 WP_407714454.1 NAD(P)-dependent oxidoreductase -
  ACJJWD_RS23360 (ACJJWD_23380) - 5149593..5150246 (-) 654 WP_003050576.1 BON domain-containing protein -
  ACJJWD_RS23365 (ACJJWD_23385) - 5150243..5150851 (-) 609 WP_003050573.1 SIS domain-containing protein -
  ACJJWD_RS23370 (ACJJWD_23390) - 5150941..5151342 (-) 402 WP_003050571.1 YraN family protein -
  ACJJWD_RS23375 (ACJJWD_23395) rsmI 5151342..5152265 (+) 924 WP_019043139.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42021.53 Da        Isoelectric Point: 6.7687

>NTDB_id=1077456 ACJJWD_RS23350 WP_003050582.1 5147336..5148472(+) (pilU) [Comamonas testosteroni strain VSPL.dLG]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSQLHTLALARSIMNDKQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1077456 ACJJWD_RS23350 WP_003050582.1 5147336..5148472(+) (pilU) [Comamonas testosteroni strain VSPL.dLG]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAATTTCCGCCCGCCATCAAGATTGACGGCAAGGTCACCAAGGTCTCGCCGCAGCCGCTGAGCCAGCTGCATA
CGCTGGCCCTGGCGCGCTCCATCATGAACGACAAGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCGTGGGGCGCTTCCGCGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCACAGGTGCTCAAGGAGGTCACCATGACCAAGCGCGGCCTGTGCA
TTCTGGTCGGCGCGACGGGCTCCGGTAAATCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAACGCGAGGTGGGCCT
GGATACCGAAAGCTGGGAAGCGGCGCTCAAAAATACGCTGCGCCAGGCTCCCGATGTGATTTTGATGGGCGAAATCCGCG
ACCGTGAAACCATGGAGCACGCGATTGCGTTCTCGGAAACCGGTCACCTGTGTCTGGCCACGCTGCATGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAACGCCGCCCACAGCTGCTGATGGATGTGTCGCTGAACCT
GCGGGCCATCATCTCCCAGCGTCTGATTCCCAAGCAGGACAGCAAGGGCCGTGTCGCCGCCATCGAGGTGATGCTCAACT
CTCCGCTGATTGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCTGGACCTGGCAGCCGGCACCGAAC
ATCTGACCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7WR82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.202

96.825

0.593
  pilU Acinetobacter baylyi ADP1

56.267

94.974

0.534
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.342

88.095

0.373
  pilT Legionella pneumophila strain Lp02

42.342

88.095

0.373
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment