Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJRPX_RS07800 Genome accession   NZ_CP174475
Coordinates   1714512..1715633 (+) Length   373 a.a.
NCBI ID   WP_411350505.1    Uniprot ID   -
Organism   Moraxella lincolnii strain CCUG 52988     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709512..1720633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJRPX_RS07775 (ACJRPX_07775) - 1710069..1710989 (-) 921 WP_078306114.1 pilin -
  ACJRPX_RS07780 (ACJRPX_07780) - 1711048..1711392 (-) 345 Protein_1502 hypothetical protein -
  ACJRPX_RS07785 (ACJRPX_07785) trmB 1711645..1712457 (+) 813 WP_143823209.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJRPX_RS07790 (ACJRPX_07790) - 1712461..1713240 (-) 780 WP_078306113.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJRPX_RS07795 (ACJRPX_07795) pilT 1713443..1714489 (+) 1047 WP_078306112.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJRPX_RS07800 (ACJRPX_07800) pilU 1714512..1715633 (+) 1122 WP_411350505.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJRPX_RS07805 (ACJRPX_07805) - 1715700..1715858 (-) 159 WP_411350506.1 hypothetical protein -
  ACJRPX_RS07810 (ACJRPX_07810) fur 1715940..1716374 (-) 435 WP_078306109.1 ferric iron uptake transcriptional regulator -
  ACJRPX_RS07815 (ACJRPX_07815) - 1716589..1716972 (+) 384 WP_078306108.1 outer membrane protein assembly factor BamE -
  ACJRPX_RS07820 (ACJRPX_07820) - 1717077..1717415 (-) 339 WP_078306107.1 RnfH family protein -
  ACJRPX_RS07825 (ACJRPX_07825) - 1717502..1718707 (-) 1206 WP_338755634.1 hypothetical protein -
  ACJRPX_RS07830 (ACJRPX_07830) - 1718931..1719596 (-) 666 WP_078306105.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41898.08 Da        Isoelectric Point: 6.3496

>NTDB_id=1077264 ACJRPX_RS07800 WP_411350505.1 1714512..1715633(+) (pilU) [Moraxella lincolnii strain CCUG 52988]
MDFDKLLQLMVKQNGSDLFITANVPPSIKINGQIMPVSKSPLSPKQTMQLVTSIMTNSQKQEFEETNECQFAISDKGETA
RFRVSAFIQRNHAGMVLRKIETEIPTVEDLNLPIILKELAMKKRGIILLVGATGTGKSTTLAAMMGYRNANSRGHIITIE
DPIEYVHEHKGCIVTQREVGIDTLSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLRAIIAQQLIPTPDGKGRRAAIEILLNSQLISDFIRKGEVHEIKPVMKRSREQGMQTFDQ
ALFDLYEAGEITYKDALKHADSPNDLRLQIKLEGKNAGQDIYDDNTLSLDINE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1077264 ACJRPX_RS07800 WP_411350505.1 1714512..1715633(+) (pilU) [Moraxella lincolnii strain CCUG 52988]
ATGGATTTTGATAAATTATTACAATTAATGGTAAAACAAAACGGCTCAGACCTATTCATTACCGCCAATGTGCCACCATC
TATAAAAATTAATGGTCAAATCATGCCTGTGAGCAAATCACCATTATCACCCAAGCAAACCATGCAGCTGGTTACCAGCA
TCATGACCAACAGCCAAAAACAAGAATTTGAAGAGACCAATGAGTGCCAGTTTGCCATTAGCGATAAAGGTGAGACAGCA
CGCTTTCGTGTCAGTGCTTTTATCCAACGAAACCATGCTGGCATGGTGCTGCGTAAAATTGAAACAGAAATCCCCACGGT
TGAAGACTTAAATCTGCCCATCATCTTAAAAGAATTGGCGATGAAAAAACGTGGCATTATCCTATTGGTCGGAGCAACAG
GCACAGGTAAATCCACCACCCTTGCCGCCATGATGGGCTATCGCAATGCCAACTCTCGAGGACACATCATCACCATCGAA
GACCCCATTGAATACGTCCACGAACACAAAGGCTGTATCGTTACTCAACGTGAAGTGGGTATTGACACCCTATCGTTTGA
AGCGGGGCTAAAAAACACCCTGCGTCAAGCCCCAGATGTGATTTTGATTGGGGAGATTCGTAACCGTGAAGTCATGAGTT
ACGCCATTCAATATGCCGAAACAGGGCATTTGGTATTTGCCACCTTGCACGCTAACAACGCCAACCAAGCCATTGACCGC
ATCATTCACTTTTTTGAGGCCGACCGTCATAATCAACTATTTATGGACTTGTCGCTCAACTTACGTGCCATCATTGCCCA
ACAGCTGATACCCACACCAGATGGCAAAGGGCGACGAGCAGCGATTGAGATTTTGTTAAATTCACAACTTATCAGTGACT
TTATTCGTAAAGGAGAAGTCCATGAAATTAAGCCTGTGATGAAGCGTTCTCGTGAGCAGGGCATGCAAACCTTTGACCAA
GCCTTGTTTGATTTGTATGAAGCAGGTGAAATCACTTACAAAGATGCCCTCAAACACGCCGATTCACCCAATGACCTACG
CCTACAAATCAAACTAGAAGGCAAAAATGCAGGTCAAGACATATATGATGACAACACATTGTCTTTAGACATTAATGAAT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.975

96.783

0.668
  pilU Acinetobacter baylyi ADP1

69.602

94.37

0.657
  pilU Vibrio cholerae strain A1552

54.416

94.102

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain Lp02

44.308

87.131

0.386
  pilT Legionella pneumophila strain ERS1305867

44.308

87.131

0.386
  pilT Pseudomonas aeruginosa PAK

41.618

92.761

0.386
  pilT Acinetobacter nosocomialis M2

41.691

91.957

0.383
  pilT Acinetobacter baumannii D1279779

41.691

91.957

0.383
  pilT Acinetobacter baumannii strain A118

41.691

91.957

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.751

92.761

0.378
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.125

85.791

0.37
  pilT Vibrio cholerae strain A1552

43.125

85.791

0.37

Multiple sequence alignment