Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACI703_RS17755 Genome accession   NZ_CP173765
Coordinates   3917011..3918141 (+) Length   376 a.a.
NCBI ID   WP_108767636.1    Uniprot ID   -
Organism   Isoptericola jiangsuensis strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3912011..3923141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS17730 (ACI703_17725) - 3912045..3913217 (-) 1173 WP_406233147.1 cystathionine gamma-synthase -
  ACI703_RS17735 (ACI703_17730) - 3913341..3914711 (-) 1371 WP_108767639.1 pyridoxal-phosphate dependent enzyme -
  ACI703_RS17740 (ACI703_17735) - 3915082..3915318 (-) 237 WP_079220527.1 YdcH family protein -
  ACI703_RS17745 (ACI703_17740) - 3915643..3916527 (-) 885 WP_406233149.1 hypothetical protein -
  ACI703_RS17750 (ACI703_17745) - 3916524..3916889 (-) 366 WP_108767637.1 DUF4398 domain-containing protein -
  ACI703_RS17755 (ACI703_17750) pilU 3917011..3918141 (+) 1131 WP_108767636.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI703_RS17760 (ACI703_17755) - 3918268..3919551 (+) 1284 WP_406233151.1 putative DNA modification/repair radical SAM protein -
  ACI703_RS17765 (ACI703_17760) - 3919532..3921004 (+) 1473 WP_406233153.1 UdgX family uracil-DNA binding protein -
  ACI703_RS17770 (ACI703_17765) - 3921054..3922991 (-) 1938 WP_406233155.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41574.87 Da        Isoelectric Point: 6.3741

>NTDB_id=1073987 ACI703_RS17755 WP_108767636.1 3917011..3918141(+) (pilU) [Isoptericola jiangsuensis strain JSH01]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMESSLEEGMESFDQCL
FRLAKAGVIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSSPTQTSITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1073987 ACI703_RS17755 WP_108767636.1 3917011..3918141(+) (pilU) [Isoptericola jiangsuensis strain JSH01]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGTGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGCTCGGCAACACCGGGCTGCCGCCGGGCATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGC
TTCCGTGTCAATGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGTTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACTTCGCTGGCGTCGATGATCGACCACCGCAACAGTACGACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGTGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGTTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAGTCGTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGCCTGGCCAAGGCCGGGGTGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAGGGTGGCAGCGGCGAGCACGATCCGTACGCGGATTTCTCCTCGCCCACCCAGACCAGCATCACCC
ACGGCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.479

0.489
  pilU Vibrio cholerae strain A1552

49.167

95.745

0.471
  pilU Acinetobacter baylyi ADP1

49.435

94.149

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.447
  pilT Pseudomonas aeruginosa PAK

42.604

89.894

0.383
  pilT Acinetobacter baumannii D1279779

41.716

89.894

0.375
  pilT Acinetobacter nosocomialis M2

41.716

89.894

0.375
  pilT Acinetobacter baumannii strain A118

41.716

89.894

0.375
  pilT Acinetobacter baylyi ADP1

41.176

90.426

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.894

0.37
  pilT Legionella pneumophila strain ERS1305867

40.828

89.894

0.367
  pilT Legionella pneumophila strain Lp02

40.828

89.894

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.564

0.362
  pilT Vibrio cholerae strain A1552

40.841

88.564

0.362

Multiple sequence alignment