Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACI3L8_RS09680 Genome accession   NZ_CP173391
Coordinates   2109948..2111054 (+) Length   368 a.a.
NCBI ID   WP_005425812.1    Uniprot ID   A7MTP8
Organism   Vibrio campbellii strain LB3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2104948..2116054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L8_RS09645 (ACI3L8_09645) - 2105088..2105690 (-) 603 WP_404971366.1 XTP/dITP diphosphatase -
  ACI3L8_RS09650 (ACI3L8_09650) - 2105796..2106227 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  ACI3L8_RS09655 (ACI3L8_09655) yggU 2106287..2106577 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  ACI3L8_RS09660 (ACI3L8_09660) - 2106577..2107134 (-) 558 WP_005425806.1 YggT family protein -
  ACI3L8_RS09665 (ACI3L8_09665) proC 2107188..2108006 (-) 819 WP_404971367.1 pyrroline-5-carboxylate reductase -
  ACI3L8_RS09670 (ACI3L8_09670) - 2108137..2108847 (-) 711 WP_005425809.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI3L8_RS09675 (ACI3L8_09675) pilT 2108876..2109916 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI3L8_RS09680 (ACI3L8_09680) pilU 2109948..2111054 (+) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI3L8_RS09685 (ACI3L8_09685) ruvX 2111116..2111541 (-) 426 WP_005425820.1 Holliday junction resolvase RuvX -
  ACI3L8_RS09690 (ACI3L8_09690) - 2111589..2112152 (-) 564 WP_038892068.1 YqgE/AlgH family protein -
  ACI3L8_RS09695 (ACI3L8_09695) gshB 2112282..2113232 (-) 951 WP_005425823.1 glutathione synthase -
  ACI3L8_RS09700 (ACI3L8_09700) rsmE 2113247..2113978 (-) 732 WP_005425825.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACI3L8_RS09705 (ACI3L8_09705) endA 2114125..2114820 (-) 696 WP_404971368.1 deoxyribonuclease I -
  ACI3L8_RS09710 (ACI3L8_09710) - 2114970..2115467 (-) 498 WP_045451129.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.48 Da        Isoelectric Point: 6.7731

>NTDB_id=1072023 ACI3L8_RS09680 WP_005425812.1 2109948..2111054(+) (pilU) [Vibrio campbellii strain LB3]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1072023 ACI3L8_RS09680 WP_005425812.1 2109948..2111054(+) (pilU) [Vibrio campbellii strain LB3]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGCGAATTGCGCCCGCAAGGTGATAAGCTGACTGAAAATGATGTCGCCTTGTTACTTGACAGTGCCA
TGGAACCAGAGCGACGCCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGATCTTGCTATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTATCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAGCACAAGCGTTGCATCGTGACACAGCGTGAAGTTGGCCTAGACACCGACAGCTATGAAGTGGCGTT
GAAAAATTCGTTACGTCAGGCGCCAGACATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTGGAGCGTATTTTGCAC
CTAGTGCCGAAAGATCAGAAAGATCAGTTCTTATTTGATCTGTCGATGAACTTAAAAGGTGTGGTGGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAACAGTCCACGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAATTGAAATCGACCATGGCTCGCTCTAACGAGTTCGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCATTGCACAGTGCAGATTCAGCCAACGATCTACGTTTAATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACAGGCACGCTGGCTAATGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.25

100

0.813
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375

Multiple sequence alignment