Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACFELC_RS24480 Genome accession   NZ_CP172849
Coordinates   5241287..5241736 (-) Length   149 a.a.
NCBI ID   WP_021264133.1    Uniprot ID   A0A509JKL4
Organism   Pseudomonas aeruginosa strain 238641E_ID020     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5236287..5246736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELC_RS24460 (ACFELC_24460) ampD 5236980..5237546 (-) 567 WP_039028111.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFELC_RS24465 (ACFELC_24465) - 5237696..5239951 (-) 2256 WP_033976391.1 DUF1631 domain-containing protein -
  ACFELC_RS24470 (ACFELC_24470) nadC 5240237..5241085 (+) 849 WP_073649707.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELC_RS24480 (ACFELC_24480) pilA 5241287..5241736 (-) 450 WP_021264133.1 pilin Machinery gene
  ACFELC_RS24485 (ACFELC_24485) pilB 5241968..5243668 (+) 1701 WP_034071567.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELC_RS24490 (ACFELC_24490) pilC 5243672..5244892 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACFELC_RS24495 (ACFELC_24495) pilD 5244896..5245768 (+) 873 WP_034071571.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELC_RS24500 (ACFELC_24500) coaE 5245765..5246376 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  ACFELC_RS24505 (ACFELC_24505) yacG 5246373..5246573 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15537.82 Da        Isoelectric Point: 7.7818

>NTDB_id=1069177 ACFELC_RS24480 WP_021264133.1 5241287..5241736(-) (pilA) [Pseudomonas aeruginosa strain 238641E_ID020]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGTNIKIGTDPSTATETYV
GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1069177 ACFELC_RS24480 WP_021264133.1 5241287..5241736(-) (pilA) [Pseudomonas aeruginosa strain 238641E_ID020]
ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTGCTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A509JKL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.358

100

0.47
  pilA Acinetobacter baumannii strain A118

47.297

99.329

0.47
  comP Acinetobacter baylyi ADP1

43.59

100

0.456
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

45.578

98.658

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

95.973

0.436
  pilA Acinetobacter nosocomialis M2

41.429

93.96

0.389
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383

Multiple sequence alignment