Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFELC_RS24490 Genome accession   NZ_CP172849
Coordinates   5243672..5244892 (+) Length   406 a.a.
NCBI ID   WP_033976387.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238641E_ID020     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5238672..5249892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELC_RS24470 (ACFELC_24470) nadC 5240237..5241085 (+) 849 WP_073649707.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELC_RS24480 (ACFELC_24480) pilA 5241287..5241736 (-) 450 WP_021264133.1 pilin Machinery gene
  ACFELC_RS24485 (ACFELC_24485) pilB 5241968..5243668 (+) 1701 WP_034071567.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELC_RS24490 (ACFELC_24490) pilC 5243672..5244892 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACFELC_RS24495 (ACFELC_24495) pilD 5244896..5245768 (+) 873 WP_034071571.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELC_RS24500 (ACFELC_24500) coaE 5245765..5246376 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  ACFELC_RS24505 (ACFELC_24505) yacG 5246373..5246573 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACFELC_RS24510 (ACFELC_24510) - 5246610..5246819 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELC_RS24515 (ACFELC_24515) - 5246925..5247614 (-) 690 WP_089034719.1 energy-coupling factor ABC transporter permease -
  ACFELC_RS24520 (ACFELC_24520) - 5247611..5248081 (-) 471 WP_033970459.1 hypothetical protein -
  ACFELC_RS24525 (ACFELC_24525) - 5248078..5248503 (-) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  ACFELC_RS24530 (ACFELC_24530) - 5248636..5249265 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACFELC_RS24535 (ACFELC_24535) - 5249262..5249711 (+) 450 WP_033970456.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44330.12 Da        Isoelectric Point: 9.8784

>NTDB_id=1069179 ACFELC_RS24490 WP_033976387.1 5243672..5244892(+) (pilC) [Pseudomonas aeruginosa strain 238641E_ID020]
MAEKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1069179 ACFELC_RS24490 WP_033976387.1 5243672..5244892(+) (pilC) [Pseudomonas aeruginosa strain 238641E_ID020]
ATGGCGGAAAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAAGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACATACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTGCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCTATCGGCGAGGAGTCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAGCCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTCGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Acinetobacter baumannii D1279779

60.494

99.754

0.603
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

36.908

98.768

0.365

Multiple sequence alignment