Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM56_RS23070 Genome accession   NZ_AP028667
Coordinates   4865578..4866714 (+) Length   378 a.a.
NCBI ID   WP_369649044.1    Uniprot ID   -
Organism   Variovorax sp. V59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4860578..4871714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM56_RS23055 (GmRootV59_45640) - 4863301..4863774 (+) 474 WP_369649042.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM56_RS23060 (GmRootV59_45650) - 4863771..4864457 (-) 687 WP_369649043.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM56_RS23065 (GmRootV59_45660) pilT 4864509..4865552 (+) 1044 WP_015867574.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM56_RS23070 (GmRootV59_45670) pilU 4865578..4866714 (+) 1137 WP_369649044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM56_RS23075 (GmRootV59_45680) - 4866949..4867857 (+) 909 WP_369649045.1 NAD(P)-dependent oxidoreductase -
  ACAM56_RS23080 (GmRootV59_45690) - 4867907..4868674 (-) 768 WP_209500981.1 BON domain-containing protein -
  ACAM56_RS23085 (GmRootV59_45700) - 4868671..4869315 (-) 645 WP_145739786.1 SIS domain-containing protein -
  ACAM56_RS23090 (GmRootV59_45710) - 4869391..4869762 (-) 372 WP_145739784.1 YraN family protein -
  ACAM56_RS23095 (GmRootV59_45720) rsmI 4869807..4870754 (+) 948 WP_369649046.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM56_RS23100 (GmRootV59_45730) - 4870761..4871303 (-) 543 WP_369649047.1 RNA 2'-phosphotransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41898.93 Da        Isoelectric Point: 6.4189

>NTDB_id=106813 ACAM56_RS23070 WP_369649044.1 4865578..4866714(+) (pilU) [Variovorax sp. V59]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106813 ACAM56_RS23070 WP_369649044.1 4865578..4866714(+) (pilU) [Variovorax sp. V59]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCCGACTTCCCGCCCGCGATCAAGGTCGATGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGGGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCCATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAAAATTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCATGCCGTGGCCTTTGCCGAAACCGGCCACCTCTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTGGACCGGATCATCAACTTCTTCCCCGAGGAACGCCGCGCCCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCGCAGCGCCTGGTGCCCACCGAGGACGGCCAGGGCCGCGTGGCGGCGGTGGAAGTGCTGCTGAACA
CGCCCCTCATTTCGGACCTGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTTGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGTCGGCCGGCACGGAGC
ATTTCGCGATCGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment