Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   ACFSN5_RS00810 Genome accession   NZ_CP172427
Coordinates   143491..144438 (+) Length   315 a.a.
NCBI ID   WP_165212397.1    Uniprot ID   -
Organism   Streptococcus sp. ZJ 1593     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 138491..149438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFSN5_RS00795 (ACFSN5_00795) - 139426..140046 (+) 621 WP_165212401.1 TetR/AcrR family transcriptional regulator -
  ACFSN5_RS00800 (ACFSN5_00800) - 140389..142764 (-) 2376 WP_165329443.1 HAD-IC family P-type ATPase -
  ACFSN5_RS00805 (ACFSN5_00805) - 142983..143357 (+) 375 WP_165329442.1 DUF1033 family protein -
  ACFSN5_RS00810 (ACFSN5_00810) comGA/cglA 143491..144438 (+) 948 WP_165212397.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFSN5_RS00815 (ACFSN5_00815) comYB 144362..145405 (+) 1044 WP_277423768.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFSN5_RS00820 (ACFSN5_00820) comYC 145402..145728 (+) 327 WP_165212393.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFSN5_RS00825 (ACFSN5_00825) comYD 145682..146116 (+) 435 WP_165212383.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFSN5_RS00830 (ACFSN5_00830) comYE 146088..146381 (+) 294 WP_165212381.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACFSN5_RS00835 (ACFSN5_00835) comYF 146365..146802 (+) 438 WP_165212379.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACFSN5_RS00840 (ACFSN5_00840) comGG 146780..147238 (+) 459 WP_165212377.1 competence type IV pilus minor pilin ComGG -
  ACFSN5_RS00845 (ACFSN5_00845) comYH 147415..148368 (+) 954 WP_165212375.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 315 a.a.        Molecular weight: 35695.97 Da        Isoelectric Point: 6.3941

>NTDB_id=1067647 ACFSN5_RS00810 WP_165212397.1 143491..144438(+) (comGA/cglA) [Streptococcus sp. ZJ 1593]
MVQVQELGKSLIGEAVSRQAQDIYLLPKIDDYEIYLRIGDERRLFQTCPQEQAMGLISHFKFVAGMNVGEKRRAQLGACH
YDFADGETAALRLSTVGDYQGRESLVVRLLFDRRRELMFWFDGLNALLPKIIDRGLYLFSGPVGSGKTTLMYELVSRKYA
DRQIITIEDPVEIKQDQMLQLQLNPSIEMTYDNLIKLSLRHRPDVLIIGEIRDKETAQAVIRASLTGIRVFSTVHAKSIP
GVYARILELGVSQEELDNCLAGIAYQRLIGGGGLIDFAQDHFQSHKADSWNKKIDQLFTTGHLTYEQAEAEKIKN

Nucleotide


Download         Length: 948 bp        

>NTDB_id=1067647 ACFSN5_RS00810 WP_165212397.1 143491..144438(+) (comGA/cglA) [Streptococcus sp. ZJ 1593]
ATGGTACAGGTGCAGGAGCTAGGGAAGAGTCTGATTGGGGAAGCTGTCAGCAGGCAGGCTCAGGATATTTATTTGCTGCC
AAAGATTGATGATTATGAAATTTATCTGCGCATAGGTGATGAGAGACGTCTATTCCAAACTTGCCCTCAGGAACAGGCTA
TGGGACTAATCAGTCATTTTAAATTTGTTGCTGGAATGAATGTTGGGGAGAAGCGGCGAGCCCAGTTGGGAGCCTGTCAC
TATGATTTTGCAGATGGTGAGACGGCCGCTCTGCGTTTATCAACGGTCGGAGACTATCAAGGACGGGAAAGTCTGGTTGT
TCGCTTGCTCTTTGACAGGCGACGAGAGCTGATGTTTTGGTTTGATGGCCTCAATGCACTGTTACCCAAGATTATTGACC
GAGGCCTCTATCTTTTCTCTGGCCCTGTTGGTTCGGGTAAGACGACTTTGATGTATGAGTTGGTCAGTCGCAAGTATGCT
GACCGCCAGATTATTACTATTGAAGATCCTGTTGAGATTAAACAGGATCAGATGCTACAGCTCCAGCTTAATCCCTCTAT
CGAGATGACCTATGATAATTTGATTAAACTTTCACTGCGACACCGGCCAGATGTTCTAATTATAGGAGAAATTCGCGATA
AAGAGACAGCCCAAGCGGTTATCAGAGCTAGCTTGACAGGAATACGAGTTTTCTCAACCGTCCATGCTAAGAGTATTCCA
GGTGTTTATGCTCGGATTTTAGAATTGGGAGTTTCTCAGGAAGAGTTGGATAATTGTCTGGCTGGCATTGCTTACCAGCG
TTTAATCGGGGGAGGAGGATTGATTGATTTTGCTCAAGACCATTTTCAAAGTCACAAGGCTGACAGCTGGAATAAGAAAA
TTGACCAGCTTTTTACAACAGGACATCTCACTTACGAGCAGGCAGAAGCCGAAAAGATTAAGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

86.349

100

0.863
  comYA Streptococcus mutans UA140

66.346

99.048

0.657
  comYA Streptococcus mutans UA159

66.346

99.048

0.657
  comYA Streptococcus gordonii str. Challis substr. CH1

64.744

99.048

0.641
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.258

98.413

0.613
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.935

98.413

0.61
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.935

98.413

0.61
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.935

98.413

0.61
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.935

98.413

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

53.226

98.413

0.524
  comGA Latilactobacillus sakei subsp. sakei 23K

40.278

91.429

0.368

Multiple sequence alignment