Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM52_RS29840 Genome accession   NZ_AP028663
Coordinates   6419233..6420369 (+) Length   378 a.a.
NCBI ID   WP_062482331.1    Uniprot ID   A0A554RS03
Organism   Variovorax sp. V118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6414233..6425369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM52_RS29810 (GmRootV118_59130) dapA 6414317..6415210 (+) 894 WP_369645354.1 4-hydroxy-tetrahydrodipicolinate synthase -
  ACAM52_RS29815 (GmRootV118_59140) - 6415217..6416059 (+) 843 WP_369645355.1 alpha/beta hydrolase -
  ACAM52_RS29820 (GmRootV118_59150) - 6416056..6416646 (+) 591 WP_369645356.1 malonic semialdehyde reductase -
  ACAM52_RS29825 (GmRootV118_59160) - 6416657..6417394 (+) 738 WP_369645357.1 sulfite exporter TauE/SafE family protein -
  ACAM52_RS29830 (GmRootV118_59170) - 6417407..6418108 (-) 702 WP_369645358.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM52_RS29835 (GmRootV118_59180) pilT 6418160..6419203 (+) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM52_RS29840 (GmRootV118_59190) pilU 6419233..6420369 (+) 1137 WP_062482331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM52_RS29845 (GmRootV118_59200) - 6420439..6421347 (+) 909 WP_369645359.1 NAD(P)-dependent oxidoreductase -
  ACAM52_RS29850 (GmRootV118_59210) - 6421378..6422172 (-) 795 WP_369645360.1 BON domain-containing protein -
  ACAM52_RS29855 (GmRootV118_59220) - 6422169..6422810 (-) 642 WP_062482322.1 SIS domain-containing protein -
  ACAM52_RS29860 (GmRootV118_59230) - 6422897..6423268 (-) 372 WP_070063810.1 YraN family protein -
  ACAM52_RS29865 (GmRootV118_59240) rsmI 6423316..6424275 (+) 960 WP_369645361.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM52_RS29870 (GmRootV118_59250) - 6424279..6425007 (-) 729 WP_369645362.1 glutathione S-transferase -
  ACAM52_RS29875 (GmRootV118_59260) - 6425097..6425324 (-) 228 WP_062481748.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41796.88 Da        Isoelectric Point: 6.3136

>NTDB_id=106759 ACAM52_RS29840 WP_062482331.1 6419233..6420369(+) (pilU) [Variovorax sp. V118]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106759 ACAM52_RS29840 WP_062482331.1 6419233..6420369(+) (pilU) [Variovorax sp. V118]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCTCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAAACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGGCGCTTTCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGATGGCCTGGGCATGCCGCAGGTGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGTTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTCGCCTTTGCTGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTCTTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTGTCGCAGCGGCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGATCCTGCTGAACA
CGCCGTTGATCTCCGACCTGATCTTTAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCGATCAACTTCGAAGAGGCGATCCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Legionella pneumophila strain ERS1305867

44.681

87.037

0.389
  pilT Legionella pneumophila strain Lp02

44.681

87.037

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment