Detailed information NTG1060694

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ACFWQH_RS23745
• Genome accession: NZ_CP171272
• Coordinates: 5042402..5042854 (-)
• Length: 150 a.a.
• NCBI ID: WP_023518088.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain W221205R
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5037402..5047854

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFWQH_RS23725 (ACFWQH_23725)	ampD	5038097..5038663 (-)	567	WP_003094640.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ACFWQH_RS23730 (ACFWQH_23730)	-	5038814..5041069 (-)	2256	WP_023518087.1	DUF1631 domain-containing protein	-
ACFWQH_RS23735 (ACFWQH_23735)	nadC	5041355..5042203 (+)	849	WP_003161760.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ACFWQH_RS23745 (ACFWQH_23745)	pilA	5042402..5042854 (-)	453	WP_023518088.1	pilin	Machinery gene
ACFWQH_RS23750 (ACFWQH_23750)	pilB	5043086..5044786 (+)	1701	WP_021264132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACFWQH_RS23755 (ACFWQH_23755)	pilC	5044790..5046010 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
ACFWQH_RS23760 (ACFWQH_23760)	pilD	5046014..5046886 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ACFWQH_RS23765 (ACFWQH_23765)	coaE	5046883..5047494 (+)	612	WP_023518089.1	dephospho-CoA kinase	-
ACFWQH_RS23770 (ACFWQH_23770)	yacG	5047491..5047691 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15686.02 Da
• Isoelectric Point: 7.7814

>NTDB_id=1060694 ACFWQH_RS23745 WP_023518088.1 5042402..5042854(-) (pilA) [Pseudomonas aeruginosa strain W221205R]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

• Length: 453 bp

>NTDB_id=1060694 ACFWQH_RS23745 WP_023518088.1 5042402..5042854(-) (pilA) [Pseudomonas aeruginosa strain W221205R]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

• Pilin (bacterial filament) (Pilin) (35-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	47.651	99.333	0.473
pilA	Vibrio cholerae strain A1552	47.651	99.333	0.473
pilA	Vibrio cholerae C6706	47.651	99.333	0.473
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.333	100	0.453
pilA	Acinetobacter baumannii strain A118	44.595	98.667	0.44
pilA	Vibrio parahaemolyticus RIMD 2210633	43.243	98.667	0.427
comP	Acinetobacter baylyi ADP1	42	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.958	95.333	0.4
pilA2	Legionella pneumophila strain ERS1305867	39.597	99.333	0.393
pilA2	Legionella pneumophila str. Paris	38.926	99.333	0.387
pilA/pilA1	Eikenella corrodens VA1	38.514	98.667	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	38.889	96	0.373
pilA	Acinetobacter nosocomialis M2	41.353	88.667	0.367