Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACF3OJ_RS11890 Genome accession   NZ_CP171111
Coordinates   2468335..2468775 (+) Length   146 a.a.
NCBI ID   WP_392406970.1    Uniprot ID   -
Organism   Cardiobacterium hominis strain WGS1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2463335..2473775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3OJ_RS11860 (ACF3OJ_11860) - 2463407..2464288 (+) 882 WP_392405838.1 DMT family transporter -
  ACF3OJ_RS11865 (ACF3OJ_11865) murI 2464285..2465103 (+) 819 WP_392405840.1 glutamate racemase -
  ACF3OJ_RS11870 (ACF3OJ_11870) - 2465226..2465816 (+) 591 WP_311336902.1 Slam-dependent surface lipoprotein -
  ACF3OJ_RS11875 (ACF3OJ_11875) - 2466170..2466940 (-) 771 WP_004140962.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACF3OJ_RS11880 (ACF3OJ_11880) - 2467014..2467502 (-) 489 WP_079539431.1 hypothetical protein -
  ACF3OJ_RS11885 (ACF3OJ_11885) - 2467499..2467942 (-) 444 WP_004140958.1 universal stress protein -
  ACF3OJ_RS11890 (ACF3OJ_11890) pilE 2468335..2468775 (+) 441 WP_392406970.1 pilin Machinery gene
  ACF3OJ_RS11895 (ACF3OJ_11895) pilE 2468853..2469296 (+) 444 WP_004140954.1 pilin Machinery gene
  ACF3OJ_RS11900 (ACF3OJ_11900) - 2469360..2472266 (+) 2907 WP_392405842.1 fused MFS/spermidine synthase -
  ACF3OJ_RS11905 (ACF3OJ_11905) - 2472306..2473235 (-) 930 WP_143312202.1 hypothetical protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15367.67 Da        Isoelectric Point: 9.3425

>NTDB_id=1060147 ACF3OJ_RS11890 WP_392406970.1 2468335..2468775(+) (pilE) [Cardiobacterium hominis strain WGS1530]
MKKLQKGFTLIELMIVIAIIGILAAIALPAYQDYIARSQMTEGFNLAGGQKGALSEFYADKGRWPSNNTEAGIAVASQIT
GKYVANVAVAGSEITATMKTSEISKGIQGKKVILTGTVAGNPQDQGSYQWACSSDASAKYLPASCR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1060147 ACF3OJ_RS11890 WP_392406970.1 2468335..2468775(+) (pilE) [Cardiobacterium hominis strain WGS1530]
ATGAAAAAATTGCAAAAAGGCTTCACCCTCATCGAGCTGATGATCGTTATCGCCATCATCGGTATTCTGGCTGCTATCGC
TCTGCCGGCTTACCAAGACTATATCGCTCGTTCACAAATGACTGAAGGTTTTAACCTTGCTGGTGGCCAAAAAGGCGCCC
TGAGCGAATTCTACGCTGACAAAGGCAGATGGCCTTCTAACAATACTGAAGCAGGTATTGCTGTAGCAAGCCAAATTACT
GGCAAATATGTTGCTAACGTTGCTGTTGCTGGTAGTGAAATTACAGCCACGATGAAAACTTCTGAAATTTCTAAGGGCAT
TCAAGGTAAAAAAGTTATCTTGACTGGTACTGTAGCAGGTAACCCGCAGGATCAAGGTTCATATCAGTGGGCTTGCTCAT
CTGACGCTTCTGCTAAATATCTTCCTGCTTCTTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

49.08

100

0.548
  pilE Neisseria gonorrhoeae MS11

48.78

100

0.548
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.842

100

0.479
  pilA/pilA1 Eikenella corrodens VA1

43.949

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

39.286

100

0.452
  pilA2 Legionella pneumophila strain ERS1305867

42.657

97.945

0.418
  pilA2 Legionella pneumophila str. Paris

42.657

97.945

0.418
  comP Acinetobacter baylyi ADP1

39.474

100

0.411
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.726

100

0.397
  pilA Acinetobacter nosocomialis M2

37.908

100

0.397
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.384
  pilA Acinetobacter baumannii strain A118

38.356

100

0.384
  pilA Vibrio campbellii strain DS40M4

42.188

87.671

0.37

Multiple sequence alignment