Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACF3OJ_RS11895 Genome accession   NZ_CP171111
Coordinates   2468853..2469296 (+) Length   147 a.a.
NCBI ID   WP_004140954.1    Uniprot ID   C8N9J4
Organism   Cardiobacterium hominis strain WGS1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2463853..2474296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3OJ_RS11865 (ACF3OJ_11865) murI 2464285..2465103 (+) 819 WP_392405840.1 glutamate racemase -
  ACF3OJ_RS11870 (ACF3OJ_11870) - 2465226..2465816 (+) 591 WP_311336902.1 Slam-dependent surface lipoprotein -
  ACF3OJ_RS11875 (ACF3OJ_11875) - 2466170..2466940 (-) 771 WP_004140962.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACF3OJ_RS11880 (ACF3OJ_11880) - 2467014..2467502 (-) 489 WP_079539431.1 hypothetical protein -
  ACF3OJ_RS11885 (ACF3OJ_11885) - 2467499..2467942 (-) 444 WP_004140958.1 universal stress protein -
  ACF3OJ_RS11890 (ACF3OJ_11890) pilE 2468335..2468775 (+) 441 WP_392406970.1 pilin Machinery gene
  ACF3OJ_RS11895 (ACF3OJ_11895) pilE 2468853..2469296 (+) 444 WP_004140954.1 pilin Machinery gene
  ACF3OJ_RS11900 (ACF3OJ_11900) - 2469360..2472266 (+) 2907 WP_392405842.1 fused MFS/spermidine synthase -
  ACF3OJ_RS11905 (ACF3OJ_11905) - 2472306..2473235 (-) 930 WP_143312202.1 hypothetical protein -
  ACF3OJ_RS11910 (ACF3OJ_11910) - 2473373..2474236 (+) 864 WP_392405843.1 spermidine synthase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15771.23 Da        Isoelectric Point: 8.9947

>NTDB_id=1060148 ACF3OJ_RS11895 WP_004140954.1 2468853..2469296(+) (pilE) [Cardiobacterium hominis strain WGS1530]
MGVKVKRNIQQGFTLIELMVVIAIIGVLAAIALPAYQIYVTRAQMGEAFNLVDAQKNAVVETRTNKGDWPSNNTEAGMGD
PTKISGKYVEKVEVTSGGVITATMKSSGIAATIQHKTLTLTPTETNGSYTWECSSNVGKMYLPAVCR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1060148 ACF3OJ_RS11895 WP_004140954.1 2468853..2469296(+) (pilE) [Cardiobacterium hominis strain WGS1530]
ATGGGTGTTAAAGTGAAAAGAAATATACAGCAAGGCTTTACGCTGATTGAGTTGATGGTCGTGATTGCTATTATTGGGGT
GTTGGCTGCTATTGCACTTCCCGCTTATCAAATTTATGTTACGCGGGCGCAAATGGGCGAAGCTTTCAATTTAGTAGATG
CGCAAAAGAACGCGGTTGTTGAAACTCGAACCAATAAAGGGGACTGGCCATCTAATAACACAGAAGCAGGTATGGGCGAC
CCTACTAAAATCAGCGGAAAATATGTTGAAAAAGTAGAAGTGACTAGCGGTGGTGTTATTACGGCTACAATGAAATCGTC
TGGAATAGCGGCTACAATACAACATAAAACGTTAACGCTTACTCCTACAGAGACCAATGGTTCATACACTTGGGAATGCA
GTTCTAACGTTGGTAAAATGTATCTTCCTGCAGTATGCAGGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8N9J4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

49.045

100

0.524
  pilE Neisseria gonorrhoeae strain FA1090

46.795

100

0.497
  pilA2 Legionella pneumophila str. Paris

42.143

95.238

0.401
  pilA2 Legionella pneumophila strain ERS1305867

42.143

95.238

0.401
  pilA/pilA1 Eikenella corrodens VA1

37.255

100

0.388
  comP Acinetobacter baylyi ADP1

37.5

100

0.388
  pilA Acinetobacter baumannii strain A118

38.732

96.599

0.374
  pilA Vibrio cholerae strain A1552

35.256

100

0.374
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.256

100

0.374
  pilA Vibrio cholerae C6706

35.256

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

97.279

0.367
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.758

100

0.361

Multiple sequence alignment