Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFYG5_RS12950 Genome accession   NZ_CP170721
Coordinates   2761412..2762524 (+) Length   370 a.a.
NCBI ID   WP_395116475.1    Uniprot ID   A0AB74UME6
Organism   Rhodanobacter sp. FW102-FHT14D07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2756412..2767524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYG5_RS12930 (ACFYG5_12930) - 2758017..2759387 (-) 1371 WP_395116481.1 pyridoxal-phosphate dependent enzyme -
  ACFYG5_RS12935 (ACFYG5_12935) - 2759539..2759766 (-) 228 WP_136257589.1 YdcH family protein -
  ACFYG5_RS12940 (ACFYG5_12940) - 2760099..2760758 (-) 660 WP_395116479.1 hypothetical protein -
  ACFYG5_RS12945 (ACFYG5_12945) - 2760755..2761339 (-) 585 WP_395116477.1 DUF4398 domain-containing protein -
  ACFYG5_RS12950 (ACFYG5_12950) pilU 2761412..2762524 (+) 1113 WP_395116475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFYG5_RS12955 (ACFYG5_12955) maiA 2762654..2763316 (-) 663 WP_395116473.1 maleylacetoacetate isomerase -
  ACFYG5_RS12960 (ACFYG5_12960) - 2763499..2764683 (+) 1185 WP_395116470.1 phosphoglycerate kinase -
  ACFYG5_RS12965 (ACFYG5_12965) - 2764683..2765336 (+) 654 WP_395116468.1 HAD hydrolase-like protein -
  ACFYG5_RS12970 (ACFYG5_12970) tkt 2765466..2767475 (-) 2010 WP_395116466.1 transketolase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41068.32 Da        Isoelectric Point: 6.5971

>NTDB_id=1058871 ACFYG5_RS12950 WP_395116475.1 2761412..2762524(+) (pilU) [Rhodanobacter sp. FW102-FHT14D07]
MDIGYFLKLMVDKGASDMFLSTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERNLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIEQLQLPPIFRDLIMEPRGLILVVGATGSGKSTTLAAMIDQRNSQSPGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNSDQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRIPAAEVLLNTPLIRDMIRRGQIHEIKDAMDRSLQEGMQTFDQSL
YKLYKEGRVELDEALSKSDSRDGLALKIRLAEGGNSSAELAGNDPYGMGF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1058871 ACFYG5_RS12950 WP_395116475.1 2761412..2762524(+) (pilU) [Rhodanobacter sp. FW102-FHT14D07]
GTGGACATCGGCTACTTCCTCAAGCTCATGGTAGACAAGGGCGCGTCGGACATGTTCCTGTCCACCGGTGCCCCGGTGAA
CATCAAGGTCGAGGGCAAGCTTTATCCGCTGGGCAATACCGGCCTGCCGAGCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAAGTGCCGCAGTTCGAGCGCAACCTCGAACTGAACATGGCATTGGCGGTGAAGGAAGCCGGCCGC
TTCCGCATCAACGTCTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGAGATCCCCTCGATCGA
GCAGCTGCAGCTGCCGCCGATCTTCCGCGACCTGATCATGGAGCCGCGCGGACTGATCCTGGTGGTGGGCGCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCAGCGCAACTCGCAGTCGCCCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGCGCTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACGCACAGCTACCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCATGATCGGCGAGATCCGCGACACCGACACGATGGAGGCGG
CCATTGCCTTCTCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACTCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAATCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGCCGCATCCCGGCCGCCGAGGTGCTGCTGAACACGCCGCTGATCCGCGACATGATTC
GCCGCGGCCAGATCCACGAGATCAAGGACGCCATGGATCGCAGTCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACAAGCTGTACAAGGAAGGCCGCGTGGAACTGGACGAGGCCCTCAGCAAATCCGACTCCCGCGATGGCCTCGCCCTCAA
GATCCGCCTCGCCGAAGGCGGCAACAGCAGCGCCGAACTGGCCGGCAACGACCCGTACGGGATGGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.852

95.135

0.484
  pilU Vibrio cholerae strain A1552

47.838

100

0.478
  pilU Acinetobacter baylyi ADP1

49.02

96.486

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

91.622

0.405
  pilT Acinetobacter baylyi ADP1

41.176

91.892

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.351

0.37
  pilT Legionella pneumophila strain ERS1305867

42.415

87.297

0.37
  pilT Legionella pneumophila strain Lp02

42.415

87.297

0.37
  pilT Pseudomonas aeruginosa PAK

40.237

91.351

0.368
  pilT Vibrio cholerae strain A1552

40.356

91.081

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

91.081

0.368
  pilT Acinetobacter baumannii strain A118

41.59

88.378

0.368
  pilT Acinetobacter baumannii D1279779

41.59

88.378

0.368
  pilT Acinetobacter nosocomialis M2

41.59

88.378

0.368

Multiple sequence alignment