Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJI2_RS00920 Genome accession   NZ_CP170549
Coordinates   204234..205424 (+) Length   396 a.a.
NCBI ID   WP_377703351.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 199234..210424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI2_RS00895 (ACFJI2_00895) - 200026..200748 (+) 723 WP_377703347.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJI2_RS00900 (ACFJI2_00900) proC 200758..201672 (+) 915 WP_377703348.1 pyrroline-5-carboxylate reductase -
  ACFJI2_RS00905 (ACFJI2_00905) - 201838..202191 (-) 354 WP_377703349.1 YqjD family protein -
  ACFJI2_RS00910 (ACFJI2_00910) ubiA 202249..203103 (-) 855 WP_377703350.1 4-hydroxybenzoate octaprenyltransferase -
  ACFJI2_RS00915 (ACFJI2_00915) - 203140..204084 (-) 945 WP_107877275.1 hydrogen peroxide-inducible genes activator -
  ACFJI2_RS00920 (ACFJI2_00920) pilU 204234..205424 (+) 1191 WP_377703351.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJI2_RS00925 (ACFJI2_00925) rfaE2 205437..205919 (+) 483 WP_377703352.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  ACFJI2_RS00930 (ACFJI2_00930) - 205921..206382 (-) 462 WP_377703353.1 DUF421 domain-containing protein -
  ACFJI2_RS00935 (ACFJI2_00935) - 206493..207356 (-) 864 WP_377703354.1 transposase -
  ACFJI2_RS00940 (ACFJI2_00940) trmL 207404..207874 (-) 471 WP_377703355.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  ACFJI2_RS00945 (ACFJI2_00945) - 207889..208719 (-) 831 WP_377703356.1 double zinc ribbon domain-containing protein -
  ACFJI2_RS00950 (ACFJI2_00950) - 208778..209722 (+) 945 WP_377705777.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 44035.27 Da        Isoelectric Point: 6.2745

>NTDB_id=1057909 ACFJI2_RS00920 WP_377703351.1 204234..205424(+) (pilU) [Pseudoduganella sp. UC29_71]
MSSTFGHEEALAYIHKLLKVMHQTGGSDLFISADFPPSIKSQGAMKPLSQQRLTGEVTRALAMAIMNDKQRNEFAAEMEC
NFAISLPDVCRFRVNVFVQQQNVAMVIRTIASEIPNFEKLDLPEVLKEVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
HSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLKAIVSQRLVRTEDGKGRKAAIEILLNTPTIAEMIFKGNFHSIKEIMAKS
RELGMCTFDQALYELYNKGYISYEEALRNADSANGLRLQIKLRGDRKEPGEGGASRAAGELSMQIDEEEEDAEPGH

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=1057909 ACFJI2_RS00920 WP_377703351.1 204234..205424(+) (pilU) [Pseudoduganella sp. UC29_71]
ATGTCATCCACATTTGGCCACGAAGAAGCGCTGGCCTACATCCACAAACTGCTCAAGGTGATGCACCAGACCGGGGGCTC
GGACCTGTTCATTTCCGCCGATTTCCCGCCCAGCATCAAGTCGCAGGGCGCCATGAAGCCGCTCAGCCAGCAACGCCTGA
CCGGCGAAGTCACGCGCGCGCTGGCCATGGCCATCATGAACGACAAGCAGCGCAACGAGTTCGCCGCCGAGATGGAATGC
AATTTCGCCATCTCGCTGCCGGACGTGTGCCGGTTCCGGGTCAATGTCTTCGTGCAGCAGCAGAACGTGGCCATGGTGAT
CCGCACCATCGCCTCCGAGATTCCGAATTTTGAAAAGCTGGACCTGCCCGAAGTGCTCAAGGAAGTCATCATGACCAAGC
GCGGGCTGGTGCTGGTGGTTGGCGGCACGGGGTCCGGCAAGTCAACCACGCTGGCGGCCATGATCGACTACCGCAACAGC
CACTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAGTATGTGCACAAGAACAAGGGCTGCCTGGTCACGCACCG
CGAAGTGGGCGTGGACACGCATTCCTGGCACAACGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAAACCATGGAGCACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGGCACCTTGCAT
GCGAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAGGAGCGGCGCAACCAGCTGCTGATGGACTT
GTCGTCCAACCTGAAGGCCATCGTCTCGCAGCGCCTGGTGCGCACCGAGGATGGCAAGGGGCGCAAGGCCGCCATCGAAA
TCCTGCTCAACACGCCGACCATCGCCGAGATGATCTTCAAGGGCAATTTCCACTCGATCAAGGAAATCATGGCCAAGTCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCGCTGTACGAGCTCTATAATAAGGGCTACATCTCCTACGAAGAAGCGCT
GCGCAACGCCGACTCGGCCAACGGCCTGCGGCTGCAGATCAAGCTGCGCGGCGACCGCAAGGAACCGGGCGAGGGCGGCG
CCAGCCGCGCGGCCGGCGAGCTGAGCATGCAAATCGACGAGGAAGAAGAAGACGCCGAACCCGGCCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.807

87.626

0.533
  pilU Acinetobacter baylyi ADP1

53.1

93.687

0.497
  pilU Vibrio cholerae strain A1552

54.545

86.111

0.47
  pilT Legionella pneumophila strain ERS1305867

45.345

84.091

0.381
  pilT Legionella pneumophila strain Lp02

45.345

84.091

0.381
  pilT Pseudomonas aeruginosa PAK

44.214

85.101

0.376
  pilT Acinetobacter baylyi ADP1

43.544

84.091

0.366
  pilT Pseudomonas stutzeri DSM 10701

43.027

85.101

0.366
  pilT Vibrio cholerae strain A1552

43.373

83.838

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.373

83.838

0.364
  pilT Acinetobacter baumannii strain A118

42.943

84.091

0.361
  pilT Acinetobacter baumannii D1279779

42.943

84.091

0.361
  pilT Acinetobacter nosocomialis M2

42.943

84.091

0.361

Multiple sequence alignment