Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEO96_RS02155 Genome accession   NZ_CP168657
Coordinates   433720..434826 (+) Length   368 a.a.
NCBI ID   WP_373433192.1    Uniprot ID   -
Organism   Vibrio anguillarum strain VA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428720..439826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEO96_RS02120 (ACEO96_02120) - 429061..429660 (-) 600 WP_017042394.1 XTP/dITP diphosphatase -
  ACEO96_RS02125 (ACEO96_02125) - 429673..430104 (-) 432 WP_170899356.1 DUF4426 domain-containing protein -
  ACEO96_RS02130 (ACEO96_02130) yggU 430147..430437 (-) 291 WP_088720811.1 DUF167 family protein YggU -
  ACEO96_RS02135 (ACEO96_02135) - 430437..430994 (-) 558 WP_010320643.1 YggT family protein -
  ACEO96_RS02140 (ACEO96_02140) proC 431065..431883 (-) 819 WP_096036776.1 pyrroline-5-carboxylate reductase -
  ACEO96_RS02145 (ACEO96_02145) - 431945..432646 (-) 702 WP_373433191.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEO96_RS02150 (ACEO96_02150) pilT 432672..433709 (+) 1038 WP_013857819.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEO96_RS02155 (ACEO96_02155) pilU 433720..434826 (+) 1107 WP_373433192.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEO96_RS02160 (ACEO96_02160) ruvX 434910..435338 (-) 429 WP_010320648.1 Holliday junction resolvase RuvX -
  ACEO96_RS02165 (ACEO96_02165) - 435421..435984 (-) 564 WP_013857816.1 YqgE/AlgH family protein -
  ACEO96_RS02170 (ACEO96_02170) gshB 436031..436984 (-) 954 WP_373433193.1 glutathione synthase -
  ACEO96_RS02175 (ACEO96_02175) rsmE 436998..437729 (-) 732 WP_013857814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEO96_RS02180 (ACEO96_02180) endA 437838..438545 (-) 708 WP_170899360.1 deoxyribonuclease I -
  ACEO96_RS02185 (ACEO96_02185) - 438677..439177 (-) 501 WP_019282289.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41413.49 Da        Isoelectric Point: 6.5073

>NTDB_id=1044808 ACEO96_RS02155 WP_373433192.1 433720..434826(+) (pilU) [Vibrio anguillarum strain VA1]
MELNQCLEGMLTQKASDLYLTVGAPVLFRVDGELRAHGEKLSQTQVMALLDAMMDEDRRREFQRTREANFAVVREIGRFR
VSAFFQRELPGAVIRRIETHIPTFEELKLPGVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEILLNSPRISDLLRRGDLHELKATMGKSKEVGMQTFDQALY
QLVVEDKISEQDALHSADSANDLRLMLKTKRGEGISSGTLDNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1044808 ACEO96_RS02155 WP_373433192.1 433720..434826(+) (pilU) [Vibrio anguillarum strain VA1]
ATGGAACTGAACCAATGTCTTGAGGGTATGTTGACTCAAAAAGCTTCCGACTTGTACCTCACCGTAGGAGCGCCAGTGTT
ATTTCGCGTCGATGGAGAGTTAAGAGCTCATGGCGAAAAGTTATCCCAAACGCAGGTGATGGCGTTGTTAGATGCCATGA
TGGATGAGGATCGCCGCCGTGAATTTCAACGTACACGTGAAGCCAATTTTGCAGTGGTGAGAGAGATTGGCCGCTTTCGT
GTCAGTGCTTTTTTCCAACGTGAGCTGCCCGGTGCGGTGATCCGCCGCATTGAAACTCATATCCCCACTTTTGAAGAATT
AAAGTTACCGGGCGTGTTGCAAGATCTCTCGATTGCAAAGCGTGGGCTGGTGCTGGTGGTCGGCGCGACGGGGTCGGGCA
AATCGACCACCATGGCGGCCATGACGGGTTATCGTAACTCGCACCGAACTGGGCATATTTTAACAGTGGAAGATCCGATT
GAGTTTGTGCATGAGCATCAGCGTTGCATTGTGACCCAGCGAGAAGTGGGGTTGGATACCGAGAGTTACGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCTGATATGATTTTAATTGGTGAGATCCGTTCACGTGAAACGATGGAATATGCGATGA
CCTTTGCCGAGACTGGTCATTTATGTATGGCAACGTTACATGCTAACAATGCCAACCAAGCGCTTGAGCGAATTTTGCAT
TTGGTCCCTAAAGAGCAAAAAGAGCAGTTTTTATTTGATCTGTCGATGAACCTCAAAGGCGTCGTGGGGCAGCAGTTGAT
CCGCGATAAAAATGGCAAGGGACGACATGGTGTGTTTGAGATTCTACTCAACAGTCCTCGAATTTCCGATTTGCTCCGCC
GCGGTGATCTGCACGAATTGAAAGCGACGATGGGCAAATCAAAAGAAGTGGGGATGCAGACTTTTGACCAAGCACTGTAT
CAACTGGTCGTTGAGGACAAAATCAGTGAACAAGATGCACTGCACAGCGCTGACTCGGCTAACGATTTACGTTTAATGTT
AAAAACAAAGCGTGGCGAAGGGATCAGCAGTGGCACATTGGATAACGTGCAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

86.957

100

0.87
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

95.109

0.408
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

40.419

90.761

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.761

0.367

Multiple sequence alignment