Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN2V_RS04855 Genome accession   NZ_CP168233
Coordinates   861079..862218 (+) Length   379 a.a.
NCBI ID   WP_003263110.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain 144-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856079..867218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2V_RS04820 (ACEN2V_04820) - 856150..856392 (+) 243 WP_003263116.1 hypothetical protein -
  ACEN2V_RS04825 (ACEN2V_04825) - 856551..857051 (+) 501 WP_003271865.1 Dps family protein -
  ACEN2V_RS04830 (ACEN2V_04830) ubiA 857125..858000 (+) 876 WP_014616181.1 4-hydroxybenzoate octaprenyltransferase -
  ACEN2V_RS04835 (ACEN2V_04835) - 857997..858275 (-) 279 WP_042548948.1 hypothetical protein -
  ACEN2V_RS04840 (ACEN2V_04840) proC 858284..859108 (-) 825 WP_014616182.1 pyrroline-5-carboxylate reductase -
  ACEN2V_RS04845 (ACEN2V_04845) - 859142..859864 (-) 723 WP_014616183.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN2V_RS04850 (ACEN2V_04850) pilT 859986..861029 (+) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN2V_RS04855 (ACEN2V_04855) pilU 861079..862218 (+) 1140 WP_003263110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN2V_RS04860 (ACEN2V_04860) - 862414..862854 (+) 441 WP_014616184.1 type IV pilin protein -
  ACEN2V_RS04865 (ACEN2V_04865) - 862858..863346 (+) 489 WP_014616185.1 GspH/FimT family pseudopilin -
  ACEN2V_RS04870 (ACEN2V_04870) pilV 863343..863933 (+) 591 WP_039547379.1 type IV pilus modification protein PilV -
  ACEN2V_RS04875 (ACEN2V_04875) - 863930..864958 (+) 1029 WP_014616187.1 PilW family protein -
  ACEN2V_RS04880 (ACEN2V_04880) - 864962..865498 (+) 537 WP_014616188.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42207.65 Da        Isoelectric Point: 6.9314

>NTDB_id=1043349 ACEN2V_RS04855 WP_003263110.1 861079..862218(+) (pilU) [Ralstonia solanacearum strain 144-1]
MLDRDAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREYEDSFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTINTKIPTLTELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIVNFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVTFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHGKRGGPADLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1043349 ACEN2V_RS04855 WP_003263110.1 861079..862218(+) (pilU) [Ralstonia solanacearum strain 144-1]
ATGCTGGACCGCGACGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACCTGTT
CATCACCGCTGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAACTGCGCGAGTACGAAGACTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACCAAGATCCCGACGCTCACCGAACTGGATCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTCGTGGTGGGGGCGACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATACGTGCACGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
GGTGGATACGGAGTCATGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCGTCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCGATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGCCGTGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAGTCCCGCGAGCAG
GGGATGGTCACGTTCGACCAGGCACTGTTCGAGCTCTATGAAGCGGACAAGATCACCTATGAAGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCACGGCAAGCGCGGCGGGCCGGCGGATCTGACGGCCGGTACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.756

93.14

0.575
  pilU Acinetobacter baylyi ADP1

54.875

94.723

0.52
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.844

87.863

0.385
  pilT Legionella pneumophila strain ERS1305867

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Vibrio cholerae strain A1552

45.741

83.641

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.741

83.641

0.383
  pilT Acinetobacter baylyi ADP1

44.72

84.96

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372
  pilT Acinetobacter nosocomialis M2

43.887

84.169

0.369
  pilT Acinetobacter baumannii D1279779

43.887

84.169

0.369
  pilT Acinetobacter baumannii strain A118

43.887

84.169

0.369

Multiple sequence alignment