Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ02_RS18985 Genome accession   NZ_CP167838
Coordinates   4370305..4371438 (+) Length   377 a.a.
NCBI ID   WP_104615100.1    Uniprot ID   A0A2S7C6U0
Organism   Xanthomonas sp. NCPPB 4468     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4365305..4376438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ02_RS18960 (ACCQ02_18960) - 4365468..4366664 (-) 1197 WP_316688729.1 cystathionine gamma-synthase -
  ACCQ02_RS18965 (ACCQ02_18965) - 4366754..4368124 (-) 1371 WP_316688731.1 pyridoxal-phosphate dependent enzyme -
  ACCQ02_RS18970 (ACCQ02_18970) - 4368363..4368581 (-) 219 WP_005991804.1 YdcH family protein -
  ACCQ02_RS18975 (ACCQ02_18975) - 4368935..4369825 (-) 891 WP_316688734.1 hypothetical protein -
  ACCQ02_RS18980 (ACCQ02_18980) - 4369822..4370205 (-) 384 WP_104615101.1 DUF4398 domain-containing protein -
  ACCQ02_RS18985 (ACCQ02_18985) pilU 4370305..4371438 (+) 1134 WP_104615100.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ02_RS18990 (ACCQ02_18990) maiA 4371720..4372382 (-) 663 WP_316688737.1 maleylacetoacetate isomerase -
  ACCQ02_RS18995 (ACCQ02_18995) - 4372426..4373484 (-) 1059 WP_316692687.1 fumarylacetoacetate hydrolase family protein -
  ACCQ02_RS19000 (ACCQ02_19000) - 4373791..4375176 (+) 1386 WP_055848080.1 DEAD/DEAH box helicase -
  ACCQ02_RS19005 (ACCQ02_19005) queD 4375329..4375682 (+) 354 WP_228323561.1 6-carboxytetrahydropterin synthase QueD -
  ACCQ02_RS19010 (ACCQ02_19010) - 4375874..4376209 (+) 336 WP_228323562.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41728.03 Da        Isoelectric Point: 6.1401

>NTDB_id=1039714 ACCQ02_RS18985 WP_104615100.1 4370305..4371438(+) (pilU) [Xanthomonas sp. NCPPB 4468]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1039714 ACCQ02_RS18985 WP_104615100.1 4370305..4371438(+) (pilU) [Xanthomonas sp. NCPPB 4468]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAACACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGTCCGATGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAGGTGGGCATGGTAATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
TGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGTTGCACTCCAACAACGCCGACCAGACCATCGAACGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCGGTGGTCTCGCAGCG
TCTGGTCAAGGGCGTGGATGGACGCCGCCGCCCCGCCACCGAGGTGTTGCTCAATACCCCGATGATCCGCGACCTGCTGC
GTCGTGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAGTCGTTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGACCGGTGAGATCGAGCAGGAAGAAGCGCTGCGTGCAGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGGCTGTCCGAAGGCGGCTCCGGCGAGCACGACCCATACGCGGATTACGAGTCGGCGTCGGCGCCGAAGATCAGTC
ACGGGTTTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7C6U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361

Multiple sequence alignment