Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   AB3464_RS21075 Genome accession   NZ_CP162519
Coordinates   4706881..4707300 (+) Length   139 a.a.
NCBI ID   WP_397457935.1    Uniprot ID   -
Organism   Pseudomonas asplenii strain UPB 526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4701881..4712300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3464_RS21050 (AB3464_20955) yacG 4702039..4702242 (-) 204 WP_010451880.1 DNA gyrase inhibitor YacG -
  AB3464_RS21055 (AB3464_20960) coaE 4702239..4702862 (-) 624 WP_019361534.1 dephospho-CoA kinase -
  AB3464_RS21060 (AB3464_20965) pilD 4702862..4703731 (-) 870 WP_019361533.1 A24 family peptidase Machinery gene
  AB3464_RS21065 (AB3464_20970) pilC 4703735..4704952 (-) 1218 WP_397457934.1 type II secretion system F family protein Machinery gene
  AB3464_RS21070 (AB3464_20975) pilB 4704956..4706656 (-) 1701 WP_397459838.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3464_RS21075 (AB3464_20980) pilA2 4706881..4707300 (+) 420 WP_397457935.1 pilin Machinery gene
  AB3464_RS21080 (AB3464_20985) - 4707380..4708795 (+) 1416 WP_397457936.1 O-antigen ligase family protein -
  AB3464_RS21090 (AB3464_20995) - 4709031..4709717 (-) 687 WP_397457937.1 helix-turn-helix transcriptional regulator -
  AB3464_RS21095 (AB3464_21000) - 4709976..4710599 (+) 624 WP_397457938.1 glutathione S-transferase family protein -
  AB3464_RS21100 (AB3464_21005) - 4710745..4712241 (+) 1497 WP_397457939.1 AlkA N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 13972.16 Da        Isoelectric Point: 8.1087

>NTDB_id=1026858 AB3464_RS21075 WP_397457935.1 4706881..4707300(+) (pilA2) [Pseudomonas asplenii strain UPB 526]
MSTQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKASEVVLAASAARTCVTEAIQSKGSKTGLDSCGTGFVATKYAT
GIAVGATTGVIVVTGAIKAAADTTVTMTPTFSATDATIITGWTCTGTPANWMPGSCRGT

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1026858 AB3464_RS21075 WP_397457935.1 4706881..4707300(+) (pilA2) [Pseudomonas asplenii strain UPB 526]
ATGAGTACTCAGAAAGGTTTTACATTGATTGAGCTGATGATTGTCGTTGCAATCATCGGTATTTTGGCTGCGGTTGCGTT
ACCCGCCTATCAAGACTACACCGCGCGTGCGAAGGCATCCGAGGTTGTACTTGCTGCCAGCGCGGCTAGAACCTGTGTAA
CCGAAGCTATTCAATCTAAAGGCTCCAAAACTGGTTTGGATAGTTGTGGTACTGGCTTTGTTGCCACTAAATATGCGACG
GGTATAGCTGTAGGTGCAACTACTGGTGTTATTGTCGTTACAGGAGCCATTAAAGCAGCAGCTGATACTACTGTGACTAT
GACTCCAACCTTTAGTGCGACGGATGCTACTATTATCACTGGCTGGACATGTACTGGTACGCCGGCAAACTGGATGCCCG
GTTCTTGCCGTGGCACATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.286

100

0.496
  pilA2 Legionella pneumophila strain ERS1305867

49.286

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

43.333

100

0.468
  comP Acinetobacter baylyi ADP1

41.722

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.86

100

0.41
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.007

100

0.396
  pilA Vibrio parahaemolyticus RIMD 2210633

40

97.122

0.388
  pilA Ralstonia pseudosolanacearum GMI1000

40.769

93.525

0.381
  pilE Neisseria gonorrhoeae strain FA1090

46.429

80.576

0.374
  pilA Glaesserella parasuis strain SC1401

36.62

100

0.374
  pilA Haemophilus influenzae 86-028NP

37.143

100

0.374

Multiple sequence alignment