Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB3464_RS21070 Genome accession   NZ_CP162519
Coordinates   4704956..4706656 (-) Length   566 a.a.
NCBI ID   WP_397459838.1    Uniprot ID   -
Organism   Pseudomonas asplenii strain UPB 526     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4699956..4711656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3464_RS21035 (AB3464_20940) - 4700297..4700926 (-) 630 WP_010451875.1 DUF1780 domain-containing protein -
  AB3464_RS21040 (AB3464_20945) - 4701046..4701744 (+) 699 WP_397457932.1 energy-coupling factor ABC transporter permease -
  AB3464_RS21045 (AB3464_20950) - 4701812..4702027 (+) 216 WP_397457933.1 hypothetical protein -
  AB3464_RS21050 (AB3464_20955) yacG 4702039..4702242 (-) 204 WP_010451880.1 DNA gyrase inhibitor YacG -
  AB3464_RS21055 (AB3464_20960) coaE 4702239..4702862 (-) 624 WP_019361534.1 dephospho-CoA kinase -
  AB3464_RS21060 (AB3464_20965) pilD 4702862..4703731 (-) 870 WP_019361533.1 A24 family peptidase Machinery gene
  AB3464_RS21065 (AB3464_20970) pilC 4703735..4704952 (-) 1218 WP_397457934.1 type II secretion system F family protein Machinery gene
  AB3464_RS21070 (AB3464_20975) pilB 4704956..4706656 (-) 1701 WP_397459838.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3464_RS21075 (AB3464_20980) pilA2 4706881..4707300 (+) 420 WP_397457935.1 pilin Machinery gene
  AB3464_RS21080 (AB3464_20985) - 4707380..4708795 (+) 1416 WP_397457936.1 O-antigen ligase family protein -
  AB3464_RS21090 (AB3464_20995) - 4709031..4709717 (-) 687 WP_397457937.1 helix-turn-helix transcriptional regulator -
  AB3464_RS21095 (AB3464_21000) - 4709976..4710599 (+) 624 WP_397457938.1 glutathione S-transferase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62752.92 Da        Isoelectric Point: 5.8615

>NTDB_id=1026857 AB3464_RS21070 WP_397459838.1 4704956..4706656(-) (pilB) [Pseudomonas asplenii strain UPB 526]
MNDIALSGLAKQLVAAELITDKNAQQAYQQAQRNRMSLVSYLVQNKLIKSREVAEIASEHFGVALLDLSNLDKESQPKGL
VSEKLIRQHHVLPLWRRGNKLFVGISDPSNHQAITDIQFSTGLNTEAILVEDDKLTDAIDKFFDNHVSGIDDIGDVDLDG
LDIESVDEKKHEAIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRLRTDGMLKEVARPPIHLASRIAARL
KVMANMDISERRKPQDGRIKMRISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPDQKELYLSALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLQNMGIPGFNIATSVSLIIAQRLARKLCAHCKKEMDIPEEAMVKEGFPT
EQIGKFKIYEPVGCDQCNGGYKGRIGIYEVVKNTSALQRLIMAEGNSIEIDEQMRKDGFNDLRTSGLRKAMQGLTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1026857 AB3464_RS21070 WP_397459838.1 4704956..4706656(-) (pilB) [Pseudomonas asplenii strain UPB 526]
ATGAACGATATTGCCCTCTCCGGTCTGGCCAAGCAATTGGTGGCGGCCGAGCTGATTACCGACAAGAACGCTCAGCAAGC
GTATCAGCAAGCCCAACGCAATCGGATGTCCCTAGTCAGCTATCTGGTCCAGAACAAGCTGATAAAAAGCCGCGAGGTAG
CAGAGATTGCCTCCGAACATTTCGGCGTTGCCCTACTCGACCTAAGCAACCTAGACAAAGAAAGCCAACCCAAGGGCCTG
GTTAGCGAGAAGCTGATACGCCAGCACCATGTGCTGCCCTTGTGGCGGCGTGGTAACAAGCTTTTTGTCGGCATATCCGA
CCCCAGCAACCATCAGGCAATCACCGACATCCAGTTCAGCACCGGCCTGAACACCGAAGCTATCCTGGTCGAGGATGACA
AGCTTACGGATGCCATCGATAAGTTCTTCGACAACCATGTCTCCGGTATCGACGACATTGGCGATGTCGACCTTGATGGC
CTTGACATCGAGTCCGTTGACGAGAAAAAACATGAAGCCATTGCTGGTCAGGACGCCGACGACGCACCGGTAGTGCGATT
CGTCAACAAGATGCTGCTGGATGCCATCAAAGGAGGATCCTCCGACCTGCACTTCGAGCCATACGAAAAAATCTACCGCG
TACGTCTGCGTACCGATGGCATGCTGAAGGAAGTCGCCCGTCCACCTATTCACCTGGCCAGCCGGATTGCAGCGCGCTTG
AAGGTGATGGCAAACATGGATATCTCCGAGCGCCGCAAACCGCAGGATGGGCGCATCAAGATGCGCATCTCGAAGACCAA
GGCTATCGATTTCCGGGTCAATACTCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGATCCTTCCAGCG
CACAGATGGGTATCGATGCGCTGGGTTATGAGCCGGATCAGAAAGAACTGTACCTTTCAGCATTGAAGCAGCCACAGGGC
ATGATTCTGGTGACCGGCCCCACCGGGTCGGGTAAAACCGTTTCGCTCTATACCGGCCTGAATATCCTCAACACCGTGGA
TATCAATATTTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGACTTCGCCCAGGCCCTGCGGTCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGTGAGATCCGTGACCTG
GAAACCGCCGAAATCGCTATCAAGGCAGCACAGACCGGCCATATGGTGCTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCAAAACATGGGCATTCCCGGTTTCAACATCGCCACCTCTGTCAGCCTGATCATTGCCCAGCGCC
TGGCGCGTAAGCTCTGCGCCCATTGCAAGAAGGAAATGGACATCCCAGAGGAAGCCATGGTCAAAGAAGGATTCCCAACA
GAGCAGATCGGCAAGTTCAAGATCTATGAACCGGTCGGTTGCGATCAATGCAACGGCGGCTATAAAGGTCGTATCGGGAT
CTACGAGGTGGTGAAGAACACCTCAGCACTGCAACGCCTGATAATGGCCGAAGGCAATTCGATCGAAATCGATGAGCAAA
TGCGCAAGGACGGCTTTAACGACCTGCGCACCTCGGGACTGCGCAAAGCCATGCAGGGGCTGACCAGCCTGGAGGAGATC
AACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Acinetobacter baylyi ADP1

57.143

100

0.572
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.293

0.496
  pilB Vibrio campbellii strain DS40M4

49.734

99.47

0.495
  pilF Neisseria gonorrhoeae MS11

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

36.673

98.763

0.362

Multiple sequence alignment