Detailed information NTG1026855

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB3464_RS21060
• Genome accession: NZ_CP162519
• Coordinates: 4702862..4703731 (-)
• Length: 289 a.a.
• NCBI ID: WP_019361533.1
• Uniprot ID: A0A1H6NA05
• Organism: Pseudomonas asplenii strain UPB 526
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4697862..4708731

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3464_RS21025 (AB3464_20930)	-	4698582..4699889 (-)	1308	WP_090202196.1	NAD(P)/FAD-dependent oxidoreductase	-
AB3464_RS21030 (AB3464_20935)	-	4699953..4700126 (-)	174	WP_010451874.1	DUF3094 family protein	-
AB3464_RS21035 (AB3464_20940)	-	4700297..4700926 (-)	630	WP_010451875.1	DUF1780 domain-containing protein	-
AB3464_RS21040 (AB3464_20945)	-	4701046..4701744 (+)	699	WP_397457932.1	energy-coupling factor ABC transporter permease	-
AB3464_RS21045 (AB3464_20950)	-	4701812..4702027 (+)	216	WP_397457933.1	hypothetical protein	-
AB3464_RS21050 (AB3464_20955)	yacG	4702039..4702242 (-)	204	WP_010451880.1	DNA gyrase inhibitor YacG	-
AB3464_RS21055 (AB3464_20960)	coaE	4702239..4702862 (-)	624	WP_019361534.1	dephospho-CoA kinase	-
AB3464_RS21060 (AB3464_20965)	pilD	4702862..4703731 (-)	870	WP_019361533.1	A24 family peptidase	Machinery gene
AB3464_RS21065 (AB3464_20970)	pilC	4703735..4704952 (-)	1218	WP_397457934.1	type II secretion system F family protein	Machinery gene
AB3464_RS21070 (AB3464_20975)	pilB	4704956..4706656 (-)	1701	WP_397459838.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AB3464_RS21075 (AB3464_20980)	pilA2	4706881..4707300 (+)	420	WP_397457935.1	pilin	Machinery gene

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31861.96 Da
• Isoelectric Point: 8.4916

>NTDB_id=1026855 AB3464_RS21060 WP_019361533.1 4702862..4703731(-) (pilD) [Pseudomonas asplenii strain UPB 526]
MPLPDFLASYPLAFVFCALILGLIVGSFINVVVWRLPKMLERDWRAQAHDVLGLPPPAHTSAYNLLLPHSHCPHCNHRIR
AWENLPLLSYVLLRGRCSSCKAPISKRYPLTELACGLLSAFIAWHFGFGWPAGLMLVLTWGLLAMSLIDADHQILPDALV
LPLLWLGLIANHFAMFTSLNDALWGAVAGYLSLWSVYWVFKLLTGKEGMGHGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGLILLRLRNAQTSTPIPFGPYLAIAGWIALLWGGQITASYLQFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=1026855 AB3464_RS21060 WP_019361533.1 4702862..4703731(-) (pilD) [Pseudomonas asplenii strain UPB 526]
ATGCCCCTACCCGATTTCCTGGCCAGCTACCCGCTGGCCTTTGTTTTTTGCGCACTCATTCTCGGCCTGATCGTCGGCAG
CTTTATCAACGTCGTCGTCTGGCGACTGCCGAAAATGCTCGAGCGCGACTGGCGCGCCCAGGCACACGATGTACTCGGCC
TGCCGCCCCCCGCCCACACCTCGGCCTATAACCTGTTGCTGCCGCATTCGCATTGTCCGCACTGCAACCATCGCATCCGA
GCCTGGGAAAACCTGCCACTGCTCAGCTACGTCCTGCTACGCGGCAGATGCTCAAGCTGCAAGGCCCCGATCAGCAAGCG
CTACCCGCTGACCGAACTGGCCTGCGGCCTGCTCTCGGCATTTATCGCCTGGCACTTCGGCTTCGGCTGGCCTGCCGGCT
TGATGCTGGTGCTGACCTGGGGGTTGCTGGCCATGAGCCTGATCGATGCCGACCACCAGATACTCCCCGACGCCCTGGTA
CTGCCCCTGCTCTGGCTGGGCCTGATCGCCAACCACTTTGCGATGTTCACCTCGCTGAACGATGCGCTGTGGGGCGCCGT
CGCCGGTTATCTGAGCCTGTGGTCGGTGTATTGGGTATTCAAGCTGTTGACCGGCAAGGAAGGCATGGGCCATGGCGACT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGCGGCTGGCAAATACTGCCGCTGACCATCCTGCTGTCGTCCCTGGTAGGC
GCGATACTCGGGCTGATTCTGCTGCGCCTGCGTAACGCCCAGACCTCGACGCCGATCCCCTTTGGCCCTTATCTGGCGAT
TGCCGGCTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGCCTCCTATCTGCAATTTGCCGGCTTCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A1H6NA05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.429	96.886	0.547
pilD	Vibrio campbellii strain DS40M4	52.982	98.616	0.522
pilD	Acinetobacter baumannii D1279779	49.286	96.886	0.478
pilD	Acinetobacter nosocomialis M2	49.286	96.886	0.478
pilD	Neisseria gonorrhoeae MS11	49.804	88.235	0.439