Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AB2S62_RS12470 Genome accession   NZ_CP162100
Coordinates   2785592..2785990 (+) Length   132 a.a.
NCBI ID   WP_367987321.1    Uniprot ID   -
Organism   Vibrio sp. NTOU-M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780592..2790990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2S62_RS12445 (AB2S62_12205) dsbC 2781316..2782107 (-) 792 WP_367987317.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  AB2S62_RS12450 (AB2S62_12210) xerD 2782122..2783021 (-) 900 WP_367989208.1 site-specific tyrosine recombinase XerD -
  AB2S62_RS12455 (AB2S62_12215) fldB 2783184..2783705 (+) 522 WP_367987318.1 flavodoxin FldB -
  AB2S62_RS12460 (AB2S62_12220) ampD 2783729..2784274 (-) 546 WP_367987319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB2S62_RS12465 (AB2S62_12225) nadC 2784406..2785308 (+) 903 WP_367987320.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB2S62_RS12470 (AB2S62_12230) pilA 2785592..2785990 (+) 399 WP_367987321.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB2S62_RS12475 (AB2S62_12235) pilB 2785994..2787682 (+) 1689 WP_367987322.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB2S62_RS12480 (AB2S62_12240) pilC 2787710..2788936 (+) 1227 WP_367987323.1 type II secretion system F family protein Machinery gene
  AB2S62_RS12485 (AB2S62_12245) pilD 2788996..2789874 (+) 879 WP_367987324.1 A24 family peptidase Machinery gene
  AB2S62_RS12490 (AB2S62_12250) coaE 2789876..2790484 (+) 609 WP_367987325.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13359.29 Da        Isoelectric Point: 10.0411

>NTDB_id=1024048 AB2S62_RS12470 WP_367987321.1 2785592..2785990(+) (pilA) [Vibrio sp. NTOU-M3]
MQTKQTRKQKGFTLIELMIVVAVIGVLAAIAIPQYQKYVEKGALGSALATATALKTNYEDYLAISGAAPSTSAAINATPF
SLGTISVTSGGIAVNVTKGGGSGSGITLNRSTQGNWSCTVSSANSVKLNGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=1024048 AB2S62_RS12470 WP_367987321.1 2785592..2785990(+) (pilA) [Vibrio sp. NTOU-M3]
ATGCAAACAAAACAAACTCGTAAACAAAAAGGTTTTACGTTAATTGAATTGATGATCGTAGTAGCTGTAATTGGTGTACT
TGCGGCGATTGCGATTCCTCAATATCAAAAATACGTTGAAAAAGGAGCTTTAGGTTCAGCACTTGCGACTGCAACCGCTC
TTAAAACAAATTACGAAGACTACCTAGCAATTAGTGGGGCAGCTCCTTCAACTTCTGCTGCAATTAACGCAACACCATTT
AGCCTTGGAACCATATCTGTAACTTCTGGTGGTATTGCTGTAAATGTAACCAAAGGCGGTGGCTCCGGTAGCGGTATAAC
TCTAAATAGAAGCACGCAAGGCAATTGGAGCTGTACAGTTAGTTCAGCAAATAGTGTGAAGCTCAATGGTTGCTCATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(45-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

46.259

100

0.515
  pilA Vibrio cholerae C6706

46.259

100

0.515
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.259

100

0.515
  pilA Pseudomonas aeruginosa PAK

41.216

100

0.462
  pilA Vibrio parahaemolyticus RIMD 2210633

39.286

100

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.41

100

0.394
  pilA Acinetobacter baumannii strain A118

37.778

100

0.386
  comP Acinetobacter baylyi ADP1

37.594

100

0.379
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.842

100

0.371
  pilA2 Legionella pneumophila str. Paris

39.344

92.424

0.364
  pilA2 Legionella pneumophila strain ERS1305867

39.344

92.424

0.364

Multiple sequence alignment