Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB2S62_RS12475 Genome accession   NZ_CP162100
Coordinates   2785994..2787682 (+) Length   562 a.a.
NCBI ID   WP_367987322.1    Uniprot ID   -
Organism   Vibrio sp. NTOU-M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780994..2792682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2S62_RS12445 (AB2S62_12205) dsbC 2781316..2782107 (-) 792 WP_367987317.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  AB2S62_RS12450 (AB2S62_12210) xerD 2782122..2783021 (-) 900 WP_367989208.1 site-specific tyrosine recombinase XerD -
  AB2S62_RS12455 (AB2S62_12215) fldB 2783184..2783705 (+) 522 WP_367987318.1 flavodoxin FldB -
  AB2S62_RS12460 (AB2S62_12220) ampD 2783729..2784274 (-) 546 WP_367987319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB2S62_RS12465 (AB2S62_12225) nadC 2784406..2785308 (+) 903 WP_367987320.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB2S62_RS12470 (AB2S62_12230) pilA 2785592..2785990 (+) 399 WP_367987321.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB2S62_RS12475 (AB2S62_12235) pilB 2785994..2787682 (+) 1689 WP_367987322.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB2S62_RS12480 (AB2S62_12240) pilC 2787710..2788936 (+) 1227 WP_367987323.1 type II secretion system F family protein Machinery gene
  AB2S62_RS12485 (AB2S62_12245) pilD 2788996..2789874 (+) 879 WP_367987324.1 A24 family peptidase Machinery gene
  AB2S62_RS12490 (AB2S62_12250) coaE 2789876..2790484 (+) 609 WP_367987325.1 dephospho-CoA kinase -
  AB2S62_RS12495 (AB2S62_12255) zapD 2790518..2791258 (+) 741 WP_367987326.1 cell division protein ZapD -
  AB2S62_RS12500 (AB2S62_12260) yacG 2791330..2791527 (+) 198 WP_367987327.1 DNA gyrase inhibitor YacG -
  AB2S62_RS12505 (AB2S62_12265) rplS 2791611..2791964 (-) 354 WP_367987328.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62193.35 Da        Isoelectric Point: 5.1935

>NTDB_id=1024049 AB2S62_RS12475 WP_367987322.1 2785994..2787682(+) (pilB) [Vibrio sp. NTOU-M3]
MLTNLPSILRQTNILSAEQEPKVEEHINASGMSVPEALVDLGIIDSQALTIELCRIFGFPEKKLTQYDYKQLCQQLSLRE
LITRHLALPIEQQGQTLTLAICDPTNLEVEDEFRFATGLQIELVLANCKDLQGAVRKLYGRTINGASSNVKDINENELAE
LVDVSDDEIGGLEDLSKDEAPVSRYINQILLDAVRKGASDIHFEPYEDSYRVRLRCDGILIETQQPSHHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNAETAIDMRVSTLPTLWGEKIVLRLLDSSAANLDINKLGYNDTQKQLYLNALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINIRGINQVQVQPQIGFGFAEALRAFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLGNMGIAPFNIASSLSLIIAQRLARKLCPQCKLPYHAPDVVLKQLNLTPDT
PIYQANCEGCSDCNSGYSGRVGIYEVMAFNNKLAQAIIQGANIQQIEGLAQSQGMQTLKESGIEKLKQGITSYAELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1024049 AB2S62_RS12475 WP_367987322.1 2785994..2787682(+) (pilB) [Vibrio sp. NTOU-M3]
ATGCTAACCAACCTTCCTTCCATCTTGCGCCAAACAAATATTCTTAGTGCGGAGCAAGAACCAAAAGTGGAAGAACATAT
CAATGCTTCAGGGATGTCCGTCCCTGAAGCATTAGTCGATCTTGGCATCATCGACAGTCAGGCATTAACCATTGAACTGT
GCCGAATTTTCGGTTTCCCAGAAAAAAAACTCACCCAATACGACTATAAGCAGCTATGCCAACAATTGTCATTGCGTGAA
CTCATTACGCGCCACCTCGCTCTTCCGATAGAGCAACAAGGGCAAACTCTCACACTGGCCATTTGTGATCCCACGAATTT
AGAAGTGGAAGACGAATTTCGCTTTGCAACAGGGCTACAAATTGAGCTAGTACTGGCAAACTGTAAAGACCTTCAAGGAG
CGGTACGTAAACTTTATGGTCGCACCATTAACGGTGCTTCATCAAATGTCAAAGACATCAATGAAAACGAACTGGCAGAG
TTAGTTGATGTCAGTGATGATGAAATTGGCGGGTTAGAAGATCTCAGTAAAGATGAAGCGCCAGTCAGTCGGTATATTAA
CCAAATTCTTCTTGATGCCGTACGTAAAGGCGCATCAGATATCCATTTTGAACCGTATGAAGACAGTTACCGGGTACGAC
TACGCTGTGATGGTATTCTGATAGAAACCCAGCAACCTTCACACCATTTAAGCCGTCGCCTTTCAGCTCGGATTAAAATT
CTGTCAAAACTCGATATTGCTGAGCGTCGACTGCCTCAAGATGGGCGGATCAAACTTCGTCTCAATGCAGAAACCGCAAT
AGATATGCGCGTTTCTACGCTCCCCACATTATGGGGTGAAAAAATCGTTCTACGACTACTCGACAGCAGCGCTGCCAATC
TTGATATCAACAAGCTTGGCTACAACGACACGCAAAAACAGCTTTATCTCAACGCCCTGAAAAAACCGCAGGGAATGATC
TTAATGACAGGCCCTACCGGAAGCGGAAAAACGGTTTCACTCTATACGGGCTTGCGCATACTCAATACTGATGAAGTCAA
CATTTCAACCGCAGAAGATCCGGTAGAGATCAACATTCGTGGTATCAACCAAGTTCAAGTTCAACCGCAAATTGGTTTTG
GCTTTGCCGAGGCTTTGCGTGCCTTCTTACGTCAGGATCCTGACATTGTGATGGTGGGCGAAATCCGCGATCTCGAAACC
GCAGAAATTGCAGTCAAAGCGGCCCAAACAGGTCACTTAGTTCTGTCAACACTCCATACAAACTCAGCGGCAGAAACTGT
GATTCGATTAGGTAACATGGGGATTGCTCCGTTCAACATCGCTTCATCACTGAGCTTGATCATTGCTCAGCGTCTTGCGC
GAAAACTTTGTCCTCAATGCAAGTTGCCGTATCACGCCCCCGATGTAGTGTTAAAACAATTAAACCTCACACCAGATACT
CCAATTTACCAAGCGAATTGTGAAGGCTGCAGCGATTGTAATAGCGGTTACTCTGGCCGAGTCGGTATCTACGAAGTGAT
GGCGTTTAATAACAAGCTCGCCCAAGCCATCATTCAAGGGGCTAATATCCAACAAATTGAAGGCCTTGCTCAGTCACAAG
GGATGCAGACACTCAAAGAGTCTGGCATTGAAAAATTAAAGCAAGGCATCACCAGTTACGCTGAATTGCAGCGTGTACTC
TACTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.488

100

0.735
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.703
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.701
  pilB Legionella pneumophila strain ERS1305867

50

97.153

0.486
  pilB Acinetobacter baumannii D1279779

52.047

91.281

0.475
  pilB Acinetobacter baylyi ADP1

51.282

90.214

0.463
  pilF Neisseria gonorrhoeae MS11

43.816

100

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

36.007

100

0.391
  pilF Thermus thermophilus HB27

38.532

96.975

0.374

Multiple sequence alignment