Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABX298_RS08960 Genome accession   NZ_CP160798
Coordinates   2054995..2055993 (-) Length   332 a.a.
NCBI ID   WP_367394322.1    Uniprot ID   -
Organism   Pseudomonas sp. X4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2049995..2060993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX298_RS08940 (ABX298_08940) argS 2051065..2052801 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  ABX298_RS08945 (ABX298_08945) - 2052803..2053501 (+) 699 WP_025340965.1 SPOR domain-containing protein -
  ABX298_RS08950 (ABX298_08950) - 2053646..2054065 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  ABX298_RS08955 (ABX298_08955) - 2054232..2054867 (+) 636 WP_025340966.1 C40 family peptidase -
  ABX298_RS08960 (ABX298_08960) pilT 2054995..2055993 (-) 999 WP_367394322.1 type IV pilus twitching motility protein PilT Machinery gene
  ABX298_RS08965 (ABX298_08965) - 2056059..2056745 (+) 687 WP_367394323.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABX298_RS08970 (ABX298_08970) proC 2056784..2057602 (+) 819 WP_367394324.1 pyrroline-5-carboxylate reductase -
  ABX298_RS08975 (ABX298_08975) - 2057613..2058203 (+) 591 WP_013974619.1 YggT family protein -
  ABX298_RS08980 (ABX298_08980) - 2058303..2059442 (+) 1140 WP_025340970.1 homoserine O-acetyltransferase -
  ABX298_RS08985 (ABX298_08985) metW 2059450..2060070 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  ABX298_RS08990 (ABX298_08990) - 2060093..2060527 (+) 435 WP_003257823.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35794.35 Da        Isoelectric Point: 6.1023

>NTDB_id=1022074 ABX298_RS08960 WP_367394322.1 2054995..2055993(-) (pilT) [Pseudomonas sp. X4]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLDGELQRMALPTLGPAALTEGMAPMLDEDQRRQWAQGDELDLALELPVLGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCSDGLILVSGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTSAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1022074 ABX298_RS08960 WP_367394322.1 2054995..2055993(-) (pilT) [Pseudomonas sp. X4]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCTGCCGCCTTGACCGAGGGCATGGCCCCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGTGCTGGGGCGC
TTTCGGCTGAACCTGTTTCGTCAGTTGCATGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGGGATGTGTTTCAAGCTGTTGCGCAGTGCAGCGATGGCTTGATCCTCGTGAGTGGGCCGACCGGCA
GCGGCAAGTCCAGCACCTTGGCGACACTGCTTGACCAACTGAACCGCGATCGGGCTCTGCATATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGTAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGTGGTGGGTTCGCCCA
GGGGTTGCGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGCGCGGCCGAGACCGGACACCTGGTGTTGGCGACTGTGCATACGCGTTCGGCGGTGAGCAGTATTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGCTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTAGCGCAGGT
GCTGGTCAGGCGTGCGGGCGGTGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGTCAGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.121

99.398

0.518
  pilT Acinetobacter baumannii D1279779

51.212

99.398

0.509
  pilT Acinetobacter nosocomialis M2

51.212

99.398

0.509
  pilT Acinetobacter baumannii strain A118

51.212

99.398

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Legionella pneumophila strain Lp02

49.091

99.398

0.488
  pilT Legionella pneumophila strain ERS1305867

49.091

99.398

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria meningitidis 8013

48.036

99.699

0.479
  pilT Neisseria gonorrhoeae MS11

47.734

99.699

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.921

99.699

0.458
  pilU Pseudomonas stutzeri DSM 10701

36.858

99.699

0.367

Multiple sequence alignment