Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB1723_RS17975 Genome accession   NZ_CP160633
Coordinates   3947584..3948714 (-) Length   376 a.a.
NCBI ID   WP_313205535.1    Uniprot ID   -
Organism   Stenotrophomonas cyclobalanopsidis strain Ed1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3942584..3953714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1723_RS17955 (AB1723_17950) - 3942921..3943910 (+) 990 WP_367381288.1 fumarylacetoacetate hydrolase family protein -
  AB1723_RS17960 (AB1723_17955) maiA 3943925..3944608 (+) 684 WP_367381289.1 maleylacetoacetate isomerase -
  AB1723_RS17965 (AB1723_17960) - 3944642..3946141 (-) 1500 WP_367381290.1 UdgX family uracil-DNA binding protein -
  AB1723_RS17970 (AB1723_17965) - 3946122..3947405 (-) 1284 WP_367381291.1 putative DNA modification/repair radical SAM protein -
  AB1723_RS17975 (AB1723_17970) pilU 3947584..3948714 (-) 1131 WP_313205535.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1723_RS17980 (AB1723_17975) - 3948836..3949201 (+) 366 WP_079220525.1 DUF4398 domain-containing protein -
  AB1723_RS17985 (AB1723_17980) - 3949198..3950082 (+) 885 WP_164082351.1 hypothetical protein -
  AB1723_RS17990 (AB1723_17985) - 3950407..3950643 (+) 237 WP_004141535.1 YdcH family protein -
  AB1723_RS17995 (AB1723_17990) - 3950986..3952356 (+) 1371 WP_367381292.1 pyridoxal-phosphate dependent enzyme -
  AB1723_RS18000 (AB1723_17995) - 3952479..3953660 (+) 1182 WP_367381293.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41570.88 Da        Isoelectric Point: 6.3741

>NTDB_id=1021737 AB1723_RS17975 WP_313205535.1 3947584..3948714(-) (pilU) [Stenotrophomonas cyclobalanopsidis strain Ed1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMESSLEEGMESFDQCL
FRLAKAGVIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSSPTQPSITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1021737 AB1723_RS17975 WP_313205535.1 3947584..3948714(-) (pilU) [Stenotrophomonas cyclobalanopsidis strain Ed1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACTGGTGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGCTCGGCAACACCGGGCTGCCGCCGGGCATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGC
TTCCGTGTCAATGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACTTCGCTGGCGTCGATGATCGACCACCGCAACAGTACGACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCAG
CCATCGCCTTCGCCGAGACCGGCCATCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCACTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAGTCGTCGCTGGAGGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGCCTGGCCAAGGCCGGGGTGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGTCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGTGGCAGCGGCGAGCACGATCCGTACGCGGATTTCTCCTCGCCCACCCAGCCCAGCATCACCC
ACGGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.479

0.489
  pilU Vibrio cholerae strain A1552

49.167

95.745

0.471
  pilU Acinetobacter baylyi ADP1

49.435

94.149

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

91.489

0.426
  pilT Pseudomonas aeruginosa PAK

42.604

89.894

0.383
  pilT Acinetobacter baumannii D1279779

41.716

89.894

0.375
  pilT Acinetobacter nosocomialis M2

41.716

89.894

0.375
  pilT Acinetobacter baumannii strain A118

41.716

89.894

0.375
  pilT Acinetobacter baylyi ADP1

41.176

90.426

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.894

0.37
  pilT Legionella pneumophila strain ERS1305867

40.828

89.894

0.367
  pilT Legionella pneumophila strain Lp02

40.828

89.894

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.564

0.362
  pilT Vibrio cholerae strain A1552

40.841

88.564

0.362

Multiple sequence alignment