Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABU614_RS11160 Genome accession   NZ_CP159925
Coordinates   2572999..2574114 (-) Length   371 a.a.
NCBI ID   WP_064749144.1    Uniprot ID   A0AAU8N0B7
Organism   Lysobacter firmicutimachus strain SR10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2567999..2579114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU614_RS11145 (ABU614_11145) - 2568622..2570598 (-) 1977 WP_363800631.1 M23 family metallopeptidase -
  ABU614_RS11150 (ABU614_11150) - 2570987..2571967 (+) 981 WP_064749142.1 fumarylacetoacetate hydrolase family protein -
  ABU614_RS11155 (ABU614_11155) maiA 2572246..2572905 (+) 660 WP_064749143.1 maleylacetoacetate isomerase -
  ABU614_RS11160 (ABU614_11160) pilU 2572999..2574114 (-) 1116 WP_064749144.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABU614_RS11165 (ABU614_11165) - 2574211..2574588 (+) 378 WP_075575217.1 DUF4398 domain-containing protein -
  ABU614_RS11170 (ABU614_11170) - 2574585..2575202 (+) 618 WP_363800634.1 hypothetical protein -
  ABU614_RS11175 (ABU614_11175) - 2575630..2575848 (+) 219 WP_064749147.1 YdcH family protein -
  ABU614_RS11180 (ABU614_11180) - 2576173..2577543 (+) 1371 WP_363800636.1 pyridoxal-phosphate dependent enzyme -
  ABU614_RS11185 (ABU614_11185) - 2577764..2578942 (+) 1179 WP_363800637.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41057.31 Da        Isoelectric Point: 6.1908

>NTDB_id=1016818 ABU614_RS11160 WP_064749144.1 2572999..2574114(-) (pilU) [Lysobacter firmicutimachus strain SR10]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPAGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSIIPSIEELQLPQVLKSIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGTDGRRLPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGSGEHDPYADMYDASATH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1016818 ABU614_RS11160 WP_064749144.1 2572999..2574114(-) (pilU) [Lysobacter firmicutimachus strain SR10]
ATGGACATCGGCTATTTCCTTAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACGACCGGCGCGCCGGTGTA
CATCAAGGTCGAGGGCCGGCTGTACCCGCTCGGCAATACCGGCCTGCCCGCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTTCCGCAGTTCGAGCGCGACCTGGAGCTCAACATGGCCCTGGCCCTGCCCGACGCGGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCATCATCCCGAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGAGCATCATCATGGCGCCGCGCGGCCTGGTCCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGATCACCGCAACAGCAACACCGCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTCGACGCCACCACCATGGAGGCGG
CGATCGCGTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCCTGGAGCGCATC
CTCAACTTCTTCCCCGAAGCCGCGCACAAGAACGTGCTGATGAACCTCGCCCTGAACCTCAAGGCGGTGGTCTCGCAGCG
CCTGGTGGTCGGCACCGACGGGCGCCGCCTGCCGGCGGCGGAAGTGCTGATCAACACTCCGCACATCCGCGACCTGATGC
GTCGCGGCCAGGTTCACGAGATCAAGCAGGCGATGGAAGAATCGCTGGAAGACGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGGCTGTACAAGGAAGGCCGGATCGAGATGGAGGAGGCGCTCAAGGCCGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCACGATCCTTACGCCGACATGTACGACGCTTCGGCGACGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

96.765

0.496
  pilU Acinetobacter baylyi ADP1

49.153

95.418

0.469
  pilU Vibrio cholerae strain A1552

48.333

97.035

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.018

91.375

0.42
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Legionella pneumophila strain Lp02

40.237

91.105

0.367
  pilT Legionella pneumophila strain ERS1305867

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

38.905

93.531

0.364
  pilT Neisseria meningitidis 8013

38.905

93.531

0.364

Multiple sequence alignment