Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABS648_RS06300 Genome accession   NZ_CP158373
Coordinates   1308316..1308762 (-) Length   148 a.a.
NCBI ID   WP_184488627.1    Uniprot ID   A0AAU7Y8L9
Organism   Pseudomonas solani strain T3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1303316..1313762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS648_RS06270 (ABS648_06255) - 1303375..1303590 (+) 216 WP_350447877.1 hypothetical protein -
  ABS648_RS06275 (ABS648_06260) yacG 1303594..1303794 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  ABS648_RS06280 (ABS648_06265) coaE 1303791..1304402 (-) 612 WP_350447878.1 dephospho-CoA kinase -
  ABS648_RS06285 (ABS648_06270) pilD 1304402..1305271 (-) 870 WP_350447879.1 A24 family peptidase Machinery gene
  ABS648_RS06290 (ABS648_06275) pilC 1305272..1306492 (-) 1221 WP_350447880.1 type II secretion system F family protein Machinery gene
  ABS648_RS06295 (ABS648_06280) pilB 1306496..1308199 (-) 1704 WP_350447881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABS648_RS06300 (ABS648_06285) pilA 1308316..1308762 (-) 447 WP_184488627.1 pilin Machinery gene
  ABS648_RS06305 (ABS648_06290) - 1308918..1310645 (+) 1728 WP_350447882.1 Wzy polymerase domain-containing protein -
  ABS648_RS06315 (ABS648_06300) nadC 1310822..1311670 (-) 849 WP_350447883.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15057.27 Da        Isoelectric Point: 9.1607

>NTDB_id=1011054 ABS648_RS06300 WP_184488627.1 1308316..1308762(-) (pilA) [Pseudomonas solani strain T3]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIARSEAASGLAALAPLKTAVEDAYARGASTTDFTLAMLGSSATASPL
GSISFPASFAADGAGSLQFNFGVLGVSGPRTTNQALRLTRTASGVWTCTSSAGLATELRPRNCATNTP

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1011054 ABS648_RS06300 WP_184488627.1 1308316..1308762(-) (pilA) [Pseudomonas solani strain T3]
ATGAAAGCACAGAAAGGCTTCACCCTGATCGAACTGATGATCGTTGTTGCGATCATCGGCATCCTCGCTGCCATCGCCAT
CCCGGCCTACCAGAACTACATTGCACGTTCTGAAGCAGCCAGTGGCTTGGCCGCTCTTGCTCCGCTCAAGACTGCAGTTG
AAGATGCCTATGCCCGTGGTGCCTCTACCACTGACTTCACCCTCGCCATGCTGGGCAGCTCCGCTACTGCAAGCCCCCTG
GGCTCCATCAGCTTCCCCGCCAGCTTCGCCGCTGACGGCGCTGGTTCTCTGCAGTTCAACTTTGGCGTTCTCGGCGTTTC
CGGCCCCCGCACCACCAACCAGGCGCTCCGCCTGACCCGCACTGCGAGTGGCGTTTGGACCTGCACCAGCAGCGCTGGCC
TTGCCACCGAACTGCGTCCGCGCAACTGCGCGACCAACACCCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

51.634

100

0.534
  pilA Acinetobacter baumannii strain A118

46.309

100

0.466
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.177

99.324

0.419
  pilA Vibrio cholerae strain A1552

42.177

99.324

0.419
  pilA Vibrio cholerae C6706

42.177

99.324

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.781

98.649

0.412
  pilA Vibrio parahaemolyticus RIMD 2210633

46.565

88.514

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

40.58

93.243

0.378
  pilA2 Legionella pneumophila str. Paris

40

94.595

0.378
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.568

93.919

0.372
  pilA2 Legionella pneumophila strain ERS1305867

38.298

95.27

0.365

Multiple sequence alignment