Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABS648_RS06295 Genome accession   NZ_CP158373
Coordinates   1306496..1308199 (-) Length   567 a.a.
NCBI ID   WP_350447881.1    Uniprot ID   A0AAU7Y6J9
Organism   Pseudomonas solani strain T3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1301496..1313199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS648_RS06255 (ABS648_06240) - 1301695..1302120 (+) 426 WP_350447874.1 GNAT family N-acetyltransferase -
  ABS648_RS06260 (ABS648_06245) - 1302117..1302584 (+) 468 WP_350447875.1 FAD/FMN-containing dehydrogenase -
  ABS648_RS06265 (ABS648_06250) - 1302581..1303267 (+) 687 WP_350447876.1 energy-coupling factor ABC transporter permease -
  ABS648_RS06270 (ABS648_06255) - 1303375..1303590 (+) 216 WP_350447877.1 hypothetical protein -
  ABS648_RS06275 (ABS648_06260) yacG 1303594..1303794 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  ABS648_RS06280 (ABS648_06265) coaE 1303791..1304402 (-) 612 WP_350447878.1 dephospho-CoA kinase -
  ABS648_RS06285 (ABS648_06270) pilD 1304402..1305271 (-) 870 WP_350447879.1 A24 family peptidase Machinery gene
  ABS648_RS06290 (ABS648_06275) pilC 1305272..1306492 (-) 1221 WP_350447880.1 type II secretion system F family protein Machinery gene
  ABS648_RS06295 (ABS648_06280) pilB 1306496..1308199 (-) 1704 WP_350447881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABS648_RS06300 (ABS648_06285) pilA 1308316..1308762 (-) 447 WP_184488627.1 pilin Machinery gene
  ABS648_RS06305 (ABS648_06290) - 1308918..1310645 (+) 1728 WP_350447882.1 Wzy polymerase domain-containing protein -
  ABS648_RS06315 (ABS648_06300) nadC 1310822..1311670 (-) 849 WP_350447883.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62343.17 Da        Isoelectric Point: 5.3378

>NTDB_id=1011053 ABS648_RS06295 WP_350447881.1 1306496..1308199(-) (pilB) [Pseudomonas solani strain T3]
MNDSPTLSGLARQLVLAELLDEKSAQNAQQQAQRNRLSLVTYVVQNKLVKSRAIAELSAEQFGVAFFDLSAIDKEGQPKD
LVSEKLLRQHRVIPLWRRGNKLFVGVSDPTNHQAVTDIQFSTGLTTEAILVEDDKLGDAIDKFFDSASSGMEDLGDVDLD
GVDIEAVDDDKQDSGAGESADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEETQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSACKKEVDVPHDTLLREGFP
EDKIGTFKIFGPVGCDNCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRKDGFNDLRNSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1011053 ABS648_RS06295 WP_350447881.1 1306496..1308199(-) (pilB) [Pseudomonas solani strain T3]
ATGAATGACTCGCCCACCCTCTCAGGTCTTGCACGCCAGTTGGTTCTCGCTGAACTACTGGATGAGAAAAGTGCGCAGAA
CGCTCAGCAACAAGCACAGCGGAATAGGCTTTCGCTGGTTACCTATGTGGTCCAGAACAAGCTGGTTAAAAGCAGAGCCA
TTGCAGAGCTGTCAGCAGAGCAGTTTGGCGTCGCTTTCTTCGACCTCAGCGCCATAGACAAGGAGGGACAGCCAAAAGAT
CTGGTTAGTGAAAAGCTGCTCCGCCAGCATCGAGTGATACCCCTCTGGCGTAGAGGTAACAAGCTATTCGTTGGAGTTTC
AGACCCTACCAATCACCAGGCTGTGACGGATATCCAGTTCAGCACAGGCCTCACTACAGAGGCGATTCTGGTCGAGGATG
ACAAGCTGGGCGATGCCATCGACAAGTTCTTCGATAGCGCCAGCTCTGGTATGGAGGACCTCGGTGACGTCGATCTGGAC
GGCGTTGATATAGAGGCGGTCGACGACGACAAACAAGACAGCGGTGCAGGTGAGAGCGCCGATGACGCTCCCGTCGTACG
CTTCGTCAATAAAATGCTGCTGGACGCCATCAAGGGCGGCTCCTCCGACTTGCACTTCGAGCCCTACGAGCGCTCGTACC
GCGTAAGGCTGCGGACCGATGGCATCCTTCACGAAGTGGCGCGCCCACCGATCCAACTGGCCAGCCGTATTTCCGCGCGC
CTGAAGGTTATGGCAGCCCTGGATATTTCCGAACGTCGCAAACCCCAGGACGGCCGGATCAAGATGAAAATTTCCAAAAC
CAAAGCCATCGACTTCCGCGTCAACACGCTGCCCACTCTCTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCATCCA
GCGCGCAGATGGGCATCGATGCGTTGGGTTATGAGGAAACACAGAAAGAACTCTATTTGAATGCACTGAAACAACCTCAG
GGCATGATCCTTGTGACGGGCCCTACAGGGTCAGGCAAGACGGTTTCGCTTTACACCGGCATCAATATTCTCAACACCGT
CGACATCAACATTTCAACAGCTGAAGACCCGGTGGAAATCAACCTGGAAGGGATCAACCAGGTCAACGTCAACCCACGTC
AGGGTATGGATTTCGCCCAAGCCCTTCGGGCTTTCCTGCGCCAAGACCCTGACGTGATCATGGTCGGCGAAATTCGTGAC
CTCGAGACCGCCGAAATCGCCATCAAGGCGGCACAGACCGGTCACATGGTGATGTCCACGCTGCACACCAACAGCGCTGC
GGAAACCTTGACCCGCCTTCGCAATATGGGCGTTCCGGCCTTCAACATCGCGACATCGGTCAATTTGATCATCGCTCAGC
GCCTGGCTCGAAAACTCTGTAGTGCATGCAAAAAGGAGGTTGATGTACCTCACGACACGCTACTCAGAGAGGGCTTCCCT
GAAGACAAGATCGGTACCTTCAAGATATTCGGCCCTGTAGGTTGTGATAATTGCAAAGGCGGTTACAAAGGCAGGGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCCTTGCAGCGCATTATCATGGAGGAAGGTAACTCCATCGATATCGCCGCGC
AGATGCGTAAAGATGGCTTCAACGATCTGCGCAACTCGGGCCTGCTGAAAGCCATGCAAGGCGTAACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.947

100

0.593
  pilB Acinetobacter baylyi ADP1

58.584

99.647

0.584
  pilB Legionella pneumophila strain ERS1305867

55.732

100

0.557
  pilB Vibrio cholerae strain A1552

50.179

98.765

0.496
  pilB Vibrio campbellii strain DS40M4

48.496

99.647

0.483
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

50.37

95.238

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.298

0.369
  pilF Thermus thermophilus HB27

36.667

100

0.369

Multiple sequence alignment