Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABHF91_RS10925 Genome accession   NZ_CP158016
Coordinates   2321882..2323027 (+) Length   381 a.a.
NCBI ID   WP_409423109.1    Uniprot ID   -
Organism   Pseudaeromonas sp. ZJS20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2316882..2328027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF91_RS10900 (ABHF91_10880) - 2317387..2318448 (+) 1062 WP_409420318.1 3-deoxy-7-phosphoheptulonate synthase -
  ABHF91_RS10905 (ABHF91_10885) - 2318445..2319194 (-) 750 WP_409420319.1 ATP-binding cassette domain-containing protein -
  ABHF91_RS10910 (ABHF91_10890) btuC 2319178..2320215 (-) 1038 WP_409420320.1 vitamin B12 ABC transporter permease BtuC -
  ABHF91_RS10915 (ABHF91_10895) - 2320300..2320659 (+) 360 WP_409420321.1 DMT family protein -
  ABHF91_RS10920 (ABHF91_10900) - 2320656..2321621 (-) 966 WP_409420322.1 calcium/sodium antiporter -
  ABHF91_RS10925 (ABHF91_10905) pilU 2321882..2323027 (+) 1146 WP_409423109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF91_RS10930 (ABHF91_10910) - 2323131..2324021 (+) 891 WP_409420323.1 DMT family transporter -
  ABHF91_RS10935 (ABHF91_10915) rsuA 2324052..2324744 (-) 693 WP_409420324.1 16S rRNA pseudouridine(516) synthase RsuA -
  ABHF91_RS10940 (ABHF91_10920) - 2324946..2326133 (+) 1188 WP_409420325.1 HD-GYP domain-containing protein -
  ABHF91_RS10945 (ABHF91_10925) putP 2326199..2327716 (-) 1518 WP_409420326.1 sodium/proline symporter PutP -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42451.64 Da        Isoelectric Point: 6.1948

>NTDB_id=1009518 ABHF91_RS10925 WP_409423109.1 2321882..2323027(+) (pilU) [Pseudaeromonas sp. ZJS20]
MDLPQMLKILATQDGSDLYLSTGAPPCAKFQGVLKTLSDQPMAPGDVAAIADAIMDSEQRIAFEKDLEMNLALSLPQVGR
FRVNIFKQRNEVSLVARNIKLDIPRFEDLKLPPILLDVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNTSGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLRAFVSQRLVKTLDGKRRAAIEIMLGTHTIRDLIKRGEFGPLKEIMEKSTALGMQTFDSSL
FKLVVEEEVISEEEALKNADSPNNLKLKIKLHREEGQLKNTGDSASWSLDPIASDEEDPFA

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1009518 ABHF91_RS10925 WP_409423109.1 2321882..2323027(+) (pilU) [Pseudaeromonas sp. ZJS20]
ATGGATCTGCCCCAGATGTTGAAAATCCTGGCCACCCAGGACGGTTCCGATCTCTATCTTTCCACCGGCGCGCCCCCCTG
CGCCAAGTTTCAAGGGGTGCTCAAGACCCTCAGTGATCAGCCCATGGCACCGGGGGACGTGGCCGCCATCGCCGACGCCA
TCATGGACAGCGAACAGCGCATCGCCTTCGAAAAAGATCTGGAGATGAACCTGGCCCTCTCCCTGCCCCAGGTGGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTCGTGGCGCGTAACATCAAGCTGGACATCCCCCGCTTCGA
GGATCTCAAGCTGCCGCCGATCCTGCTGGACGTGGTGATGGAGAAGCGGGGCCTGGTGCTGTTTGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCACTGGCGGCCCTCATCGACCACCGCAACCGCAACACCAGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAATATGTGCACCGCCACAAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCTTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAAATCCGCGACCGGGAGACCATGGAACACG
CCCTGGCCTTCTCCGAAACCGGTCACCTGGCCATCTCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCAGAGGAGCGGCGGCCCCAGCTGCTCAACGACCTGGGCAACAACCTGCGGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCCTGGACGGCAAGCGCCGCGCCGCCATCGAGATCATGCTGGGCACCCACACCATCCGGGACCTCATCA
AGCGCGGCGAGTTCGGCCCCTTGAAGGAGATCATGGAAAAATCCACCGCCCTGGGGATGCAGACCTTCGACAGCTCCTTG
TTCAAGCTGGTGGTGGAGGAAGAGGTGATCAGCGAGGAGGAGGCCCTGAAGAACGCCGACTCCCCCAACAACCTCAAGCT
CAAGATCAAGCTGCACCGGGAGGAGGGTCAGTTGAAGAACACCGGCGACTCCGCCAGCTGGAGCCTGGATCCCATCGCCA
GCGACGAAGAGGATCCCTTCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.648

93.176

0.509
  pilU Acinetobacter baylyi ADP1

51.404

93.438

0.48
  pilU Vibrio cholerae strain A1552

48.148

99.213

0.478
  pilT Pseudomonas stutzeri DSM 10701

41.449

90.551

0.375
  pilT Pseudomonas aeruginosa PAK

41.449

90.551

0.375
  pilT Legionella pneumophila strain ERS1305867

42.388

87.927

0.373
  pilT Legionella pneumophila strain Lp02

42.388

87.927

0.373
  pilT Acinetobacter nosocomialis M2

40.87

90.551

0.37
  pilT Acinetobacter baumannii strain A118

40.87

90.551

0.37
  pilT Acinetobacter baumannii D1279779

40.87

90.551

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.751

90.814

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

87.402

0.367
  pilT Vibrio cholerae strain A1552

42.042

87.402

0.367
  pilT Acinetobacter baylyi ADP1

40.29

90.551

0.365

Multiple sequence alignment