Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNP46_RS13240 Genome accession   NZ_CP157875
Coordinates   2883850..2884989 (+) Length   379 a.a.
NCBI ID   WP_349918383.1    Uniprot ID   -
Organism   Aeromonas veronii strain Ichip_C_3-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2878850..2889989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP46_RS13210 (ABNP46_13200) - 2879164..2880228 (+) 1065 WP_349918374.1 3-deoxy-7-phosphoheptulonate synthase -
  ABNP46_RS13215 (ABNP46_13205) aac(6') 2880235..2880693 (+) 459 WP_349918376.1 aminoglycoside 6'-N-acetyltransferase -
  ABNP46_RS13220 (ABNP46_13210) - 2880716..2881501 (-) 786 WP_349918377.1 ATP-binding cassette domain-containing protein -
  ABNP46_RS13225 (ABNP46_13215) btuC 2881488..2882495 (-) 1008 WP_349918378.1 vitamin B12 ABC transporter permease BtuC -
  ABNP46_RS13230 (ABNP46_13220) - 2882577..2883326 (-) 750 WP_349918380.1 transporter substrate-binding domain-containing protein -
  ABNP46_RS13235 (ABNP46_13225) - 2883440..2883796 (+) 357 WP_349918382.1 DMT family protein -
  ABNP46_RS13240 (ABNP46_13230) pilU 2883850..2884989 (+) 1140 WP_349918383.1 type IVa pilus ATPase TapW Machinery gene
  ABNP46_RS13245 (ABNP46_13235) - 2885098..2885340 (-) 243 WP_349918385.1 FeoC-like transcriptional regulator -
  ABNP46_RS13250 (ABNP46_13240) feoB 2885384..2887654 (-) 2271 WP_349918386.1 Fe(2+) transporter permease subunit FeoB -
  ABNP46_RS13255 (ABNP46_13245) - 2887651..2887878 (-) 228 WP_349918387.1 FeoA family protein -
  ABNP46_RS13260 (ABNP46_13250) - 2887997..2888326 (+) 330 WP_349918388.1 stress response translation initiation inhibitor YciH -
  ABNP46_RS13265 (ABNP46_13255) - 2888593..2889150 (+) 558 WP_349918390.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42263.32 Da        Isoelectric Point: 6.5119

>NTDB_id=1008757 ABNP46_RS13240 WP_349918383.1 2883850..2884989(+) (pilU) [Aeromonas veronii strain Ichip_C_3-7]
MELRDMLQTLAKQDGSDLYLSTGAPPCAKFNGALRPLSETPLGPGDVAKIADDIMDAEQKQQFEKELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNHSGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRAQLLNDLGNNLQAFVSQRLVKTLDGGRRAAVEIMLGTHTIRDLIKRGEFASLKEIMEKSRALGMVTFDTAL
FDLVVEGAIDEEEALKNADSANNLRLKIKLWREKGQILGSADASSWSLEPTKDGQQDLL

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1008757 ABNP46_RS13240 WP_349918383.1 2883850..2884989(+) (pilU) [Aeromonas veronii strain Ichip_C_3-7]
ATGGAATTGCGAGACATGTTACAGACGCTGGCCAAGCAGGATGGCTCCGACCTCTATCTCTCCACCGGCGCTCCCCCGTG
CGCCAAGTTCAACGGCGCACTGCGCCCCTTGAGCGAGACCCCCCTCGGGCCCGGAGACGTGGCGAAGATTGCCGACGACA
TCATGGATGCGGAGCAAAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTCGGCCGC
TTTCGCATCAACATCTTCAAGCAGCGCAACGAAGTCTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAGCTCCCTCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACGGGCT
CGGGCAAGTCGACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACCATAGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAGAACACCCTGCGCCAGGCACCGGATGTCATCCTCATCGGGGAAATTCGTGACCGCGAGACCATGGAACATG
CCCTCGCCTTCTCCGAGACCGGGCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGGCCCAGCTGCTCAACGATCTGGGCAACAACCTGCAGGCGTTCGTCTCCCAGCG
GCTGGTGAAGACGCTGGACGGCGGACGGCGGGCGGCGGTGGAGATCATGCTCGGCACCCACACCATTCGGGATCTCATCA
AGCGTGGCGAGTTTGCCAGCCTCAAGGAGATCATGGAGAAATCCCGGGCCCTTGGCATGGTCACCTTCGACACCGCCCTG
TTCGATCTGGTGGTGGAAGGGGCCATCGATGAAGAGGAGGCGCTGAAGAACGCCGACTCCGCCAACAACCTGCGACTCAA
GATCAAACTGTGGCGGGAGAAGGGGCAGATCCTCGGCAGCGCCGATGCCAGCAGCTGGAGCCTGGAACCCACCAAGGATG
GGCAGCAGGATCTCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

98.945

0.538
  pilU Acinetobacter baylyi ADP1

51.451

100

0.515
  pilU Vibrio cholerae strain A1552

49.062

98.417

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.926

93.14

0.39
  pilT Pseudomonas aeruginosa PAK

42.151

90.765

0.383
  pilT Legionella pneumophila strain ERS1305867

43.413

88.127

0.383
  pilT Legionella pneumophila strain Lp02

43.413

88.127

0.383
  pilT Vibrio cholerae strain A1552

42.643

87.863

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.643

87.863

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.279

90.765

0.375
  pilT Acinetobacter baumannii D1279779

41.916

88.127

0.369
  pilT Acinetobacter baumannii strain A118

41.916

88.127

0.369
  pilT Acinetobacter nosocomialis M2

41.916

88.127

0.369
  pilT Acinetobacter baylyi ADP1

40.116

90.765

0.364

Multiple sequence alignment