Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNP31_RS24590 Genome accession   NZ_CP157874
Coordinates   5526383..5527381 (-) Length   332 a.a.
NCBI ID   WP_085663461.1    Uniprot ID   -
Organism   Pseudomonas asiatica strain C-B8A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5521383..5532381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP31_RS24570 (ABNP31_24560) argS 5522453..5524189 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  ABNP31_RS24575 (ABNP31_24565) - 5524191..5524889 (+) 699 WP_025340965.1 SPOR domain-containing protein -
  ABNP31_RS24580 (ABNP31_24570) - 5525034..5525453 (-) 420 WP_350012846.1 TM2 domain-containing protein -
  ABNP31_RS24585 (ABNP31_24575) - 5525620..5526255 (+) 636 WP_085663462.1 C40 family peptidase -
  ABNP31_RS24590 (ABNP31_24580) pilT 5526383..5527381 (-) 999 WP_085663461.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNP31_RS24595 (ABNP31_24585) - 5527447..5528133 (+) 687 WP_085663460.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNP31_RS24600 (ABNP31_24590) proC 5528172..5528990 (+) 819 WP_085663459.1 pyrroline-5-carboxylate reductase -
  ABNP31_RS24605 (ABNP31_24595) - 5529001..5529591 (+) 591 WP_013974619.1 YggT family protein -
  ABNP31_RS24610 (ABNP31_24600) - 5529691..5530830 (+) 1140 WP_085663458.1 homoserine O-acetyltransferase -
  ABNP31_RS24615 (ABNP31_24605) metW 5530838..5531458 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  ABNP31_RS24620 (ABNP31_24610) - 5531481..5531915 (+) 435 WP_025340971.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35730.36 Da        Isoelectric Point: 6.3074

>NTDB_id=1008736 ABNP31_RS24590 WP_085663461.1 5526383..5527381(-) (pilT) [Pseudomonas asiatica strain C-B8A]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLDGELQRMALPTLGPAALTEGMAPMLDEDQRRQWAQGDELDLALELPVLGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCSDGLILVGGPTGSGKTSTLATLLDQLNRGRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1008736 ABNP31_RS24590 WP_085663461.1 5526383..5527381(-) (pilT) [Pseudomonas asiatica strain C-B8A]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCTGCCGCCTTGACCGAGGGCATGGCACCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGTGCTGGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGCATGGCCCTGCGGCCACCTTCCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGGGATGTGTTTCAAGCTGTTGCGCAATGCAGCGATGGCTTGATCCTCGTGGGTGGGCCGACCGGCA
GCGGCAAGACCAGCACCTTGGCGACACTGCTTGACCAGCTGAACCGCGGTCGGGCTCTGCATATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGTAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGTGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGTCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGCGCGGCCGAGACCGGACACCTGGTGCTGGCGACCGTGCATACGCGTTCGGCGGTGAGCAGTATTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGCTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTGGCGCAGGT
GCTGGTCAGGCGTGCGGGCGGTGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.121

99.398

0.518
  pilT Acinetobacter baumannii D1279779

51.212

99.398

0.509
  pilT Acinetobacter nosocomialis M2

51.212

99.398

0.509
  pilT Acinetobacter baumannii strain A118

51.212

99.398

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Legionella pneumophila strain Lp02

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.788

99.398

0.485
  pilT Neisseria meningitidis 8013

48.036

99.699

0.479
  pilT Neisseria gonorrhoeae MS11

47.734

99.699

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.921

99.699

0.458
  pilU Pseudomonas stutzeri DSM 10701

36.858

99.699

0.367

Multiple sequence alignment