Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABLV49_RS00390 Genome accession   NZ_CP157675
Coordinates   75905..77053 (-) Length   382 a.a.
NCBI ID   WP_349279617.1    Uniprot ID   A0AAU7LTQ2
Organism   Polaromonas hydrogenivorans strain DSM 17735     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70905..82053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLV49_RS00370 (ABLV49_00370) - 72689..72982 (+) 294 WP_349279610.1 hypothetical protein -
  ABLV49_RS00375 (ABLV49_00375) - 72985..74001 (+) 1017 WP_349279611.1 AsmA-like C-terminal region-containing protein -
  ABLV49_RS00380 (ABLV49_00380) - 74121..74501 (+) 381 WP_349279613.1 hypothetical protein -
  ABLV49_RS00385 (ABLV49_00385) - 74541..75827 (-) 1287 WP_349279615.1 solute carrier family 23 protein -
  ABLV49_RS00390 (ABLV49_00390) pilU 75905..77053 (-) 1149 WP_349279617.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABLV49_RS00395 (ABLV49_00395) - 77295..78554 (+) 1260 WP_349279619.1 transposase -
  ABLV49_RS00400 (ABLV49_00400) - 78588..79832 (+) 1245 WP_349279621.1 efflux RND transporter periplasmic adaptor subunit -
  ABLV49_RS00405 (ABLV49_00405) - 79851..81113 (+) 1263 WP_349279623.1 FtsX-like permease family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42151.55 Da        Isoelectric Point: 6.5775

>NTDB_id=1008041 ABLV49_RS00390 WP_349279617.1 75905..77053(-) (pilU) [Polaromonas hydrogenivorans strain DSM 17735]
MPLPSPAAPSPSSSDPDDLGRYLALMVAHDASDLFLSAEVAASLKIQGRIRRLPEPPLTGTHIKRLAYSVMRDSQVREFE
ADLECDLAIAMDALGRFRVNVFRQRGEVAVVVRHINSTIPTLESLKLPPVLKTLAMLKRGLVLVVGAAGSGKSTTLAAML
DYRNNMASDHILTVEDPMEFEHSHKKCIVDQREVGLDTRSFDEALRRAMREAPDVIMIGEIRDRETMQHAMAYAETGHLC
VSTLHANNANQAIQRVLNFFPETAQRQVLMDLSLNLKGVVGQRLLAGAAGKRVPAVEVMLLSPYVADLIQKGQTDEIKAV
MAKSVELGMATFDQSLYELYVRGDITHEQAIEHADSKTDLSLRIRLASGRRPDTEALQVLRD

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=1008041 ABLV49_RS00390 WP_349279617.1 75905..77053(-) (pilU) [Polaromonas hydrogenivorans strain DSM 17735]
ATGCCACTACCCTCCCCAGCCGCCCCATCCCCATCTTCGTCCGATCCCGATGACCTCGGGCGCTACCTGGCGCTGATGGT
GGCCCATGACGCGTCCGACCTTTTCCTGTCGGCTGAGGTGGCCGCCTCGCTCAAGATCCAGGGCCGGATACGCCGCCTGC
CCGAGCCGCCGCTGACGGGAACCCACATCAAGCGACTGGCGTATTCGGTGATGCGCGACAGCCAGGTTCGCGAGTTCGAG
GCCGATCTGGAGTGCGACCTGGCGATTGCCATGGATGCGCTGGGCCGCTTTCGCGTCAATGTGTTCCGCCAGCGCGGCGA
GGTGGCAGTGGTGGTGCGGCACATCAACAGCACGATTCCGACGCTCGAATCGCTGAAACTGCCGCCGGTTCTGAAAACGC
TGGCCATGCTCAAGCGCGGCCTGGTTCTGGTGGTGGGCGCGGCCGGCTCGGGCAAATCGACCACGCTGGCGGCCATGCTG
GACTACCGCAACAACATGGCCAGCGACCATATCCTGACGGTGGAAGACCCGATGGAGTTCGAGCACAGCCACAAGAAATG
CATCGTTGACCAGCGCGAGGTCGGGCTGGACACGCGCTCTTTCGACGAAGCCCTGCGCCGCGCCATGCGCGAAGCGCCCG
ACGTGATCATGATCGGCGAAATCCGCGACCGCGAGACCATGCAGCACGCCATGGCCTATGCCGAAACCGGCCACCTGTGC
GTCTCGACGCTGCATGCCAACAATGCCAACCAGGCGATCCAGCGCGTGCTGAACTTTTTCCCGGAAACCGCGCAGCGCCA
GGTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTCGTCGGCCAGCGCCTGCTGGCGGGCGCGGCTGGCAAGCGGGTTC
CGGCGGTCGAGGTGATGCTGCTGTCGCCCTATGTCGCCGATTTGATCCAGAAGGGCCAGACCGACGAGATCAAGGCGGTG
ATGGCCAAGAGCGTCGAGCTGGGCATGGCCACCTTCGACCAGTCGCTGTACGAGCTGTATGTGCGCGGCGACATCACGCA
CGAGCAGGCCATCGAGCATGCCGACTCGAAAACCGACCTGTCGCTGCGCATCCGGCTGGCGTCCGGACGCCGCCCCGACA
CCGAAGCCCTGCAGGTGCTGCGCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(28-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.441

91.361

0.497
  pilU Vibrio cholerae strain A1552

50.685

95.55

0.484
  pilU Acinetobacter baylyi ADP1

48.58

92.147

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.675

88.482

0.395
  pilT Legionella pneumophila strain ERS1305867

43.402

89.267

0.387
  pilT Legionella pneumophila strain Lp02

43.402

89.267

0.387
  pilT Acinetobacter baylyi ADP1

44.681

86.126

0.385
  pilT Acinetobacter nosocomialis M2

44.144

87.173

0.385
  pilT Acinetobacter baumannii D1279779

44.144

87.173

0.385
  pilT Acinetobacter baumannii strain A118

44.144

87.173

0.385
  pilT Pseudomonas aeruginosa PAK

43.62

88.22

0.385
  pilT Vibrio cholerae strain A1552

42.197

90.576

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.197

90.576

0.382
  pilT Pseudomonas stutzeri DSM 10701

42.433

88.22

0.374

Multiple sequence alignment