Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFV80_RS02265 Genome accession   NZ_CP156659
Coordinates   481211..482347 (+) Length   378 a.a.
NCBI ID   WP_095550180.1    Uniprot ID   -
Organism   Vandammella animalimorsus strain SK20231019-0001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476211..487347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV80_RS02245 (ABFV80_000449) - 476821..477747 (+) 927 WP_347485648.1 LD-carboxypeptidase -
  ABFV80_RS02250 (ABFV80_000450) - 478140..479087 (+) 948 WP_347485649.1 tripartite tricarboxylate transporter substrate-binding protein -
  ABFV80_RS02255 (ABFV80_000451) pilT 479381..480424 (+) 1044 WP_095542791.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFV80_RS02260 (ABFV80_000452) - 480491..481141 (+) 651 WP_124858275.1 cyclic nucleotide-binding domain-containing protein -
  ABFV80_RS02265 (ABFV80_000453) pilU 481211..482347 (+) 1137 WP_095550180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFV80_RS02270 (ABFV80_000454) - 482418..483350 (+) 933 WP_347485650.1 NAD(P)-dependent oxidoreductase -
  ABFV80_RS02275 (ABFV80_000455) aroQ 483614..484093 (+) 480 WP_347485652.1 type II 3-dehydroquinate dehydratase -
  ABFV80_RS02280 (ABFV80_000456) - 484214..484855 (+) 642 WP_095542796.1 DUF6806 family protein -
  ABFV80_RS02285 (ABFV80_000457) murI 485015..485845 (-) 831 WP_347485654.1 glutamate racemase -
  ABFV80_RS02290 (ABFV80_000458) - 486115..486576 (+) 462 WP_095550336.1 low molecular weight protein-tyrosine-phosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41816.09 Da        Isoelectric Point: 7.0679

>NTDB_id=1001886 ABFV80_RS02265 WP_095550180.1 481211..482347(+) (pilU) [Vandammella animalimorsus strain SK20231019-0001]
MERDQASKFINDLLRLMVSRNGSDLFITADFPPAIKVDGKVSKVSPQPLTPAHTLALARSIMSDKQAAEFERTKEANFAI
SPAGVGRFRVNAFVQQGHVGLVLRVIPLNVPTIDGLGLPQVLKDVAMSKRGLCILVGATGSGKSTSLAAMVDWRNEHSYG
HIVTIEDPVEFVHAHKNCVITQREIGLDTDNWEVALKNSLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQAMDRIINFFPEERRAQLLMDVSLNLRAIMSQRLIPRQDGKGRIAAIEVLLNTPRVADLIFKGEVAEIKEAMKLSRNQG
MQTFDQALFDLFEANVISYEDALRNADSLNDLRLQIKLHSTRARTKDLAAGTEHLSVV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1001886 ABFV80_RS02265 WP_095550180.1 481211..482347(+) (pilU) [Vandammella animalimorsus strain SK20231019-0001]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTGCGGCTGATGGTCAGCCGCAATGGCAGCGATTTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCTCCAAGGTCTCGCCGCAGCCGCTGACGCCGGCGCACA
CGCTGGCGCTGGCGCGCTCCATCATGAGCGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAGGCCAATTTCGCCATC
TCGCCCGCGGGCGTGGGGCGCTTTCGCGTCAACGCCTTCGTGCAGCAAGGTCACGTGGGCTTGGTGCTGCGCGTCATCCC
GCTGAATGTGCCCACCATCGACGGCCTGGGCCTGCCGCAGGTCCTCAAGGACGTGGCCATGAGCAAGCGCGGCCTGTGCA
TTTTGGTGGGCGCCACCGGCTCGGGCAAGTCCACCTCGCTGGCGGCCATGGTGGATTGGCGCAACGAGCATTCCTACGGC
CACATCGTCACCATCGAGGATCCGGTGGAATTCGTGCACGCGCACAAGAACTGCGTCATCACCCAGCGCGAGATCGGCCT
GGACACCGACAACTGGGAGGTGGCGCTGAAGAACTCGCTGCGCCAGGCGCCGGACGTGATCCTCATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCTCC
AACCAGGCCATGGACCGCATCATCAACTTCTTCCCTGAGGAGCGCCGCGCCCAGCTGCTGATGGACGTGTCTCTGAACCT
GCGCGCCATCATGTCGCAGCGTCTGATCCCGCGCCAGGACGGCAAGGGGCGCATCGCTGCGATCGAGGTGCTGCTCAACA
CCCCGCGCGTGGCCGATTTGATCTTCAAGGGCGAGGTGGCTGAGATCAAGGAAGCCATGAAGCTCAGCCGCAACCAGGGC
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAGGCCAACGTCATCAGCTACGAAGATGCGCTGCGCAATGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCACGCGCGCGCGCACCAAGGACTTGGCCGCTGGCACCGAAC
ACCTCTCTGTGGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.931

92.063

0.579
  pilU Acinetobacter baylyi ADP1

57.429

92.593

0.532
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Pseudomonas stutzeri DSM 10701

41.246

89.153

0.368
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365
  pilT Acinetobacter baylyi ADP1

41.441

88.095

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.265

87.831

0.362
  pilT Vibrio cholerae strain A1552

41.265

87.831

0.362

Multiple sequence alignment