Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABFU60_RS05095 Genome accession   NZ_CP155949
Coordinates   1216669..1217067 (+) Length   132 a.a.
NCBI ID   WP_104570927.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain bglFP 6815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1205115..1228465 1216669..1217067 within 0


Gene organization within MGE regions


Location: 1205115..1228465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU60_RS05040 (ABFU60_05030) - 1205115..1205261 (+) 147 WP_076054137.1 hypothetical protein -
  ABFU60_RS05045 (ABFU60_05035) - 1205445..1205840 (+) 396 WP_042594933.1 hypothetical protein -
  ABFU60_RS05050 (ABFU60_05040) - 1205931..1206323 (+) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  ABFU60_RS05055 (ABFU60_05045) glgX 1206849..1208978 (-) 2130 WP_274299339.1 glycogen debranching protein GlgX -
  ABFU60_RS05060 (ABFU60_05050) rimK 1209472..1210347 (+) 876 WP_076054142.1 30S ribosomal protein S6--L-glutamate ligase -
  ABFU60_RS05065 (ABFU60_05055) - 1210997..1211674 (+) 678 WP_003490678.1 response regulator transcription factor -
  ABFU60_RS05070 (ABFU60_05060) - 1211667..1213001 (+) 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
  ABFU60_RS05075 (ABFU60_05065) - 1213100..1213355 (-) 256 Protein_986 SymE family type I addiction module toxin -
  ABFU60_RS05080 (ABFU60_05070) coaE 1213549..1214172 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  ABFU60_RS05085 (ABFU60_05075) - 1214186..1215049 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU60_RS05090 (ABFU60_05080) pilC 1215056..1216318 (-) 1263 WP_104570928.1 type II secretion system F family protein Machinery gene
  ABFU60_RS05095 (ABFU60_05085) pilA/pilAI 1216669..1217067 (+) 399 WP_104570927.1 pilin Machinery gene
  ABFU60_RS05100 (ABFU60_05090) - 1217168..1217590 (+) 423 WP_104570926.1 pilin -
  ABFU60_RS05105 (ABFU60_05095) - 1217649..1219469 (+) 1821 WP_147308815.1 hypothetical protein -
  ABFU60_RS05110 (ABFU60_05100) - 1219593..1220351 (+) 759 WP_274299340.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  ABFU60_RS05115 (ABFU60_05105) - 1220848..1221534 (+) 687 WP_258872788.1 glycosyltransferase family 9 protein -
  ABFU60_RS05120 (ABFU60_05110) - 1221537..1222379 (-) 843 WP_147308817.1 glycosyltransferase -
  ABFU60_RS05125 (ABFU60_05115) - 1222373..1223545 (-) 1173 WP_181920810.1 glycosyltransferase -
  ABFU60_RS05130 (ABFU60_05120) - 1223530..1224393 (-) 864 WP_116921534.1 glycosyltransferase -
  ABFU60_RS05135 (ABFU60_05125) - 1224443..1225294 (-) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  ABFU60_RS05140 (ABFU60_05130) pilB 1225450..1227183 (+) 1734 WP_274299341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU60_RS05145 (ABFU60_05135) - 1227366..1228103 (-) 738 WP_274299342.1 zeta toxin family protein -
  ABFU60_RS05150 (ABFU60_05140) - 1228103..1228459 (-) 357 WP_139328384.1 hypothetical protein -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13656.88 Da        Isoelectric Point: 8.8372

>NTDB_id=1000110 ABFU60_RS05095 WP_104570927.1 1216669..1217067(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain bglFP 6815]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQYEVLVNEGTVPTTAAQIGLTTPTARCAV
VVSTSGITCTLNGNANKIKGKTISLNRSSADGTWSCVTGGTMDAKYKPAGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=1000110 ABFU60_RS05095 WP_104570927.1 1216669..1217067(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain bglFP 6815]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTAGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
ACCGATGTATCAGGACTATGTTGCCAAGTCGCAGGCGACTGCCGGTCTGGCAGAAATCACTCCTGGCAAGACGCAGTATG
AAGTGTTGGTCAACGAAGGTACTGTTCCTACGACGGCCGCCCAGATTGGCTTGACAACGCCGACTGCGCGTTGCGCTGTT
GTCGTAAGCACCTCTGGCATTACATGCACCTTGAATGGCAACGCGAACAAGATTAAAGGCAAAACCATTAGCCTTAACCG
TAGTTCCGCAGATGGTACTTGGAGCTGTGTAACTGGCGGCACCATGGACGCGAAATACAAGCCCGCCGGCTGTTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

58.394

100

0.606
  pilA Acinetobacter baumannii strain A118

51.064

100

0.545
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.667

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.47
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.447
  pilA2 Legionella pneumophila str. Paris

43.066

100

0.447
  comP Acinetobacter baylyi ADP1

39.456

100

0.439
  pilA Vibrio cholerae strain A1552

39.583

100

0.432
  pilA Vibrio cholerae C6706

39.583

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.432
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.409
  pilE Neisseria gonorrhoeae MS11

34.416

100

0.402
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

94.697

0.394
  pilA Haemophilus influenzae Rd KW20

38.346

100

0.386

Multiple sequence alignment