TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family higB (T6088) Predicted domain RelE
Locus tag orf4325 Length 103 a.a.
Coordinates 4635076..4635387 (-)

Antitoxin (Protein)


Predicted family higA (AT6088) Predicted domain PRK10072 (HTH)
Locus tag orf4324 Length 96 a.a.
Coordinates 4634785..4635075 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf4317 4630272..4630907 + 636 ORF4317 Predicted ORF by PRODIGAL Version 1.20 -
orf4318 4630904..4631833 + 930 ORF4318 Predicted ORF by PRODIGAL Version 1.20 -
orf4319 4632053..4632163 - 111 ORF4319 Predicted ORF by PRODIGAL Version 1.20 -
orf4320 4632196..4633404 - 1209 ORF4320 Predicted ORF by PRODIGAL Version 1.20 -
orf4321 4633474..4633743 - 270 ORF4321 Predicted ORF by PRODIGAL Version 1.20 -
orf4322 4633962..4634180 - 219 ORF4322 Predicted ORF by PRODIGAL Version 1.20 -
orf4323 4634607..4634699 - 93 ORF4323 Predicted ORF by PRODIGAL Version 1.20 -
orf4324 4634785..4635075 - 291 ORF4324 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf4325 4635076..4635387 - 312 ORF4325 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf4326 4635616..4636524 + 909 ORF4326 Predicted ORF by PRODIGAL Version 1.20 -
orf4327 4636588..4637529 - 942 ORF4327 Predicted ORF by PRODIGAL Version 1.20 -
orf4328 4637574..4638011 - 438 ORF4328 Predicted ORF by PRODIGAL Version 1.20 -
orf4329 4638008..4638880 - 873 ORF4329 Predicted ORF by PRODIGAL Version 1.20 -
orf4330 4638874..4639473 - 600 ORF4330 Predicted ORF by PRODIGAL Version 1.20 -
orf4331 4639572..4640357 - 786 ORF4331 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

RelE (E-value: 0.00036, Score:18.7)

(41-98)

Antitoxin

PRK10072 (HTH) (E-value: 6.1e-09, Score:34.3)

(37-94)

TIGR03070 (couple_hipB) (E-value: 1.3e-05, Score:22.6)

(199-262)

MqsA_antitoxin (MqsA) (E-value: 5.3e-05, Score:21.4)

(57-125)

cd00093 (Xre) (E-value: 8.1e-05, Score:20.2)

(50-110)

smart00530 (HTH_XRE) (E-value: 0.00069, Score:17.8)

(19-76)

COG1476 (XRE) (E-value: 0.0015, Score:15.5)

(16-54)

YdaS_antitoxin (YdaS) (E-value: 0.011, Score:13.8)

(10-44)

Xre-like-HTH (Xre) (E-value: 0.021, Score:13.3)

(15-53)

PF13560 (HTH_31) (E-value: 0.013, Score:12.7)

(21-57)

COG4197 (YdaS) (E-value: 0.032, Score:12.3)

(16-53)

PF01381 (HTH_3) (E-value: 0.008, Score:11.9)

(187-236)

COG4197 (YdaS) (E-value: 72, Score:1.5)

(17-41)

Xre-like-HTH (Xre) (E-value: 190, Score:0.6)

(18-40)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>ORF4325 TA_31 Toxin
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAG
VRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>ORF4325 TA_31 Toxin
TTACCACCTCGTATTTAACAACCGGAGCATTGCTTTTTCCTGGGGAGACAGATCATCTTT
AATACCCTTACGATAAATAAGCAGTAATCTGATCTCATAATGTTTAACCTGATGAAAGTA
AATAACCCTGACACCAGCACGTTTCCCCTTTCCTCCTGACACCCATCGAACCTTTCTTAA
CCCACCAGTTTCAGGTATTACCTCACCATAGTCAGGATTCAAGGCCAGGAAAAACTGAAA
ACGACTGAATTCATCGTCGGTCAGCAGTTTTTGAACATCTTCAGTAAAAATTTCCGTTTC
GATGAAGAGCAT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>ORF4324 TA_31 Antitoxin
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVD
TLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR

Download         Length: 291 bp

>ORF4324 TA_31 Antitoxin
CTAACGGTTAAGTGCTTGCAGCACATGTTGCGGATCATTGGCAATCGCTCGCAGCAAAGC
CTTTGCAGGCCCGGTCGGTGAACGTCGCCCCTGCTCCCAGTTACGCAACGTGTCTACGCT
TACAGAAATAAGATCGGCAAATTTAGATTGTGACAACCCTGAAGCGCGGCGTATTTCTTT
GATTTTCATAGCATCAACTTCAAAAGTCCGCGAAGGCTGTCGCGTTCCTTCAATAATTTC
ATTCATCTGGGCCATGCTGTGAGTCAACCGCTCAAAAAGTGCCTTATCCAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6088 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

70.874

100

0.709
T303 Pseudomonas putida KT2440

52.427

99.038

0.524
T174 Vibrio cholerae O1 biovar El Tor str. N16961

41.176

92.727

0.408
T169 Vibrio cholerae O1 biovar El Tor str. N16961

31.507

73.737

0.223