Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mqsRA (relBE)/Xre-YgiU |
| Location | 4750051..4750751 | Replicon | chromosome |
| Accession | NC_002947 | ||
| Organism | Pseudomonas putida KT2440 | ||
Toxin (Protein)
| Gene name | mqsR | Uniprot ID | Q88F93 |
| Locus tag | PP_4205 | Protein ID | WP_010954960.1 |
| Coordinates | 4750455..4750751 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | mqsA | Uniprot ID | I7AUJ7 |
| Locus tag | PP_4204 | Protein ID | WP_003254235.1 |
| Coordinates | 4750051..4750452 (-) | Length | 134 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PP_RS21810 (PP_4200) | 4745185..4746006 | - | 822 | WP_010954957.1 | transporter substrate-binding domain-containing protein | - |
| PP_RS21815 (PP_4201) | 4746082..4747011 | - | 930 | WP_010954958.1 | FAD-binding protein | - |
| PP_RS21820 (PP_4202) | 4747012..4747761 | - | 750 | WP_003254232.1 | electron transfer flavoprotein subunit beta/FixA family protein | - |
| PP_RS21825 (PP_4203) | 4748297..4749979 | + | 1683 | WP_010954959.1 | electron transfer flavoprotein-ubiquinone oxidoreductase | - |
| PP_RS21830 (PP_4204) | 4750051..4750452 | - | 402 | WP_003254235.1 | type II toxin-antitoxin system MqsA family antitoxin | Antitoxin |
| PP_RS21835 (PP_4205) | 4750455..4750751 | - | 297 | WP_010954960.1 | type II toxin-antitoxin system MqsR family toxin | Toxin |
| PP_RS21840 (PP_4206) | 4750846..4752171 | - | 1326 | WP_010954961.1 | MFS transporter | - |
| PP_RS21845 (PP_4207) | 4752244..4752723 | - | 480 | WP_010954962.1 | hypothetical protein | - |
| PP_RS21850 (PP_4208) | 4752892..4753368 | - | 477 | WP_010954963.1 | sigma-70 family RNA polymerase sigma factor | - |
| PP_RS21855 (PP_4209) | 4753581..4754759 | + | 1179 | WP_010954964.1 | efflux RND transporter periplasmic adaptor subunit | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11133.22 Da Isoelectric Point: 10.2271
>T314 WP_010954960.1 NC_002947:c4750751-4750455 [Pseudomonas putida KT2440]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL
Download Length: 297 bp
>T314 NC_002947:c4750751-4750455 [Pseudomonas putida KT2440]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA
Antitoxin
Download Length: 134 a.a. Molecular weight: 14684.76 Da Isoelectric Point: 4.9531
>AT314 WP_003254235.1 NC_002947:c4750452-4750051 [Pseudomonas putida KT2440]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA
Download Length: 402 bp
>AT314 NC_002947:c4750452-4750051 [Pseudomonas putida KT2440]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T162 | Xylella fastidiosa 9a5c |
57 |
100 |
0.582 |
| T1045 | Escherichia coli str. K-12 substr. MG1655 |
57.732 |
98.98 |
0.571 |
| T6100 | Escherichia coli str. K-12 substr. MG1655 |
57.732 |
98.98 |
0.571 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT162 | Xylella fastidiosa 9a5c |
62.121 |
99.248 |
0.617 |
| AT6100 | Escherichia coli str. K-12 substr. MG1655 |
45.038 |
100 |
0.45 |
| AT1045 | Escherichia coli str. K-12 substr. MG1655 |
45.038 |
100 |
0.45 |
Multiple sequence alignment
References
(1) Chenglong Sun et al. (2017) MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440. Frontiers in Microbiology 8:840. [PubMed:28536573]