Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mqsRA (relBE)/Xre-YgiU |
| Location | 3167851..3168544 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | mqsR | Uniprot ID | Q46865 |
| Locus tag | b3022 | Protein ID | NP_417494.1 |
| Coordinates | 3168248..3168544 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | mqsA | Uniprot ID | Q46864 |
| Locus tag | b3021 | Protein ID | NP_417493.1 |
| Coordinates | 3167851..3168246 (-) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b3019 | 3163715..3165973 | - | 2259 | NP_417491.1 | DNA topoisomerase IV subunit A | - |
| b3020 | 3166111..3167718 | - | 1608 | NP_417492.1 | putative deoxycholate binding periplasmic protein | - |
| b3021 | 3167851..3168246 | - | 396 | NP_417493.1 | antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA | Antitoxin |
| b3022 | 3168248..3168544 | - | 297 | NP_417494.1 | mRNA interferase toxin MqsR | Toxin |
| b3023 | 3168749..3169231 | - | 483 | YP_026197.1 | DNA-binding transcriptional repressor YgiV | - |
| b3024 | 3169284..3169676 | - | 393 | NP_417496.1 | BOF family protein YgiW | - |
| b3025 | 3169828..3170487 | + | 660 | NP_417497.1 | DNA-binding transcriptional activator QseB | - |
| b3026 | 3170484..3171833 | + | 1350 | NP_417498.1 | sensor histidine kinase QseC | - |
| b3027 | 3171879..3172211 | - | 333 | NP_417499.1 | DUF2645 domain-containing inner membrane protein YgiZ | - |
| b3028 | 3172530..3173111 | + | 582 | NP_417500.1 | NADPH:quinone oxidoreductase MdaB | - |
| b3029 | 3173142..3173456 | + | 315 | NP_417501.1 | putative quinol monooxygenase YgiN | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11231.96 Da Isoelectric Point: 8.9070
>T6100 NP_417494.1 NC_000913:c3168544-3168248 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK
Download Length: 297 bp
>T6100 NC_000913:c3168544-3168248 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 132 a.a. Molecular weight: 14703.10 Da Isoelectric Point: 9.2136
>AT6100 NP_417493.1 NC_000913:c3168246-3167851 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR
Download Length: 396 bp
>AT6100 NC_000913:c3168246-3167851 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1045 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| T162 | Xylella fastidiosa 9a5c |
60.606 |
100 |
0.612 |
| T314 | Pseudomonas putida KT2440 |
57.732 |
98.98 |
0.571 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1045 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| AT314 | Pseudomonas putida KT2440 |
45.038 |
100 |
0.45 |
| AT162 | Xylella fastidiosa 9a5c |
41.985 |
100 |
0.42 |
Multiple sequence alignment
References
(1) Toomas Mets et al. (2019) Fragmentation of Escherichia coli mRNA by MazF and MqsR. Biochimie 156:79-91. [PubMed:30315853]
(2) Villu Kasari et al. (2010) The Escherichia coli mqsR and ygiT genes encode a new toxin-antitoxin pair. Journal of Bacteriology 192(11):2908-19. [PubMed:20233923]