Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/Xre-YgiU
Location 2371566..2372272 Replicon chromosome
Accession NC_002488
Organism Xylella fastidiosa 9a5c

Toxin (Protein)


Gene name mqsR Uniprot ID Q9PAL4
Locus tag XF_RS10790 Protein ID WP_010894925.1
Coordinates 2371566..2371868 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID Q9PAL3
Locus tag XF_RS10795 Protein ID WP_010894926.1
Coordinates 2371871..2372272 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XF_RS10765 (XF2485) 2366724..2367902 - 1179 WP_042462912.1 phage tail sheath family protein -
XF_RS10770 (XF2486) 2367967..2369559 - 1593 WP_010894924.1 tail fiber domain-containing protein -
XF_RS10775 (XF2487) 2369567..2370124 - 558 WP_010893249.1 phage tail protein I -
XF_RS10780 (XF2488) 2370117..2371010 - 894 WP_010893248.1 baseplate J/gp47 family protein -
XF_RS10785 (XF2489) 2371010..2371348 - 339 WP_042462908.1 GPW/gp25 family protein -
XF_RS10790 (XF2490) 2371566..2371868 + 303 WP_010894925.1 type II toxin-antitoxin system MqsR family toxin Toxin
XF_RS10795 (XF2491) 2371871..2372272 + 402 WP_010894926.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
XF_RS10800 (XF2492) 2372341..2372928 - 588 WP_010894927.1 phage baseplate assembly protein V -
XF_RS10805 (XF2493) 2372925..2373467 - 543 WP_010894928.1 hypothetical protein -
XF_RS10810 (XF2494) 2373443..2373964 - 522 WP_010893241.1 hypothetical protein -
XF_RS10815 (XF2495) 2373961..2374284 - 324 WP_042462902.1 hypothetical protein -
XF_RS10820 (XF2496) 2374284..2374544 - 261 WP_010893239.1 hypothetical protein -
XF_RS10825 (XF2497) 2374562..2376442 - 1881 WP_010893238.1 phage major capsid protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2361169..2404268 43099


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(2-99)

Antitoxin

Cro/C1-type helix-turn-helix domain

(74-127)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 10958.74 Da        Isoelectric Point: 8.0251

>T162 WP_010894925.1 NC_002488:2371566-2371868 [Xylella fastidiosa 9a5c]
MEKGTPHYKLPVVKALVKAGKVRATATAYQGARELGIKDLTGMCTVVLALTSADFYKSMTTYADHTVFQDVYHAKTASGD
EVYLKLTVIDDVLIVSFKEL

Download         Length: 303 bp

>T162 NC_002488:2371566-2371868 [Xylella fastidiosa 9a5c]
ATGGAGAAAGGCACACCCCACTACAAGCTACCAGTCGTCAAAGCCTTGGTAAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTTGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 15018.15 Da        Isoelectric Point: 7.1650

>AT162 WP_010894926.1 NC_002488:2371871-2372272 [Xylella fastidiosa 9a5c]
MRCPCCGAAELIHDTRDMLYTYKSETTSIPTVTGDFCPACGEVVLDREHGDRYSELVGLFQRQVNSAYVDPGYITKIRRK
LDLDQRQAAELFGGGVNAFSRYENGKTKPPLSLVKLFKLLDRHPDLLNEVKSF

Download         Length: 402 bp

>AT162 NC_002488:2371871-2372272 [Xylella fastidiosa 9a5c]
ATGAGATGTCCATGCTGCGGCGCGGCAGAACTGATCCACGACACGCGCGACATGCTTTACACCTATAAGAGCGAAACTAC
TAGTATCCCAACGGTGACCGGCGACTTCTGCCCGGCGTGTGGCGAAGTCGTCTTGGACCGTGAACATGGTGATCGTTACA
GCGAATTGGTCGGCTTGTTCCAACGTCAGGTGAATTCGGCCTACGTCGATCCTGGTTACATTACCAAGATACGCCGGAAG
CTTGATCTCGACCAGCGGCAAGCTGCCGAACTGTTCGGTGGTGGTGTTAATGCTTTCTCACGTTACGAGAACGGTAAAAC
CAAGCCGCCGCTGTCTTTGGTTAAGTTATTCAAGCTTCTGGATCGCCACCCCGATTTGCTCAACGAAGTGAAGAGTTTCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6100 Escherichia coli str. K-12 substr. MG1655

60.606

100

0.612
T1045 Escherichia coli str. K-12 substr. MG1655

60.606

100

0.612
T314 Pseudomonas putida KT2440

57

100

0.582

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT314 Pseudomonas putida KT2440

62.121

99.248

0.617
AT6100 Escherichia coli str. K-12 substr. MG1655

41.985

100

0.42
AT1045 Escherichia coli str. K-12 substr. MG1655

41.985

100

0.42

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q9PAL4

Antitoxin

Source ID Structure
AlphaFold DB Q9PAL3

References

(1) Isis Gabriela Barbosa Carvalho et al. (2021) Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance. Frontiers in Microbiology 12:712564. [PubMed:34616378] experimental

(2) Marcus V Merfa et al. (2016) The MqsRA Toxin-Antitoxin System from Xylella fastidiosa Plays a Key Role in Bacterial Fitness, Pathogenicity, and Persister Cell Formation. Frontiers in Microbiology 7:904. [PubMed:27375608] experimental

(3) Reinaldo Rodrigues de Souza-Neto et al. (2022) MqsR toxin as a biotechnological tool for plant pathogen bacterial control. Scientific Reports 12(1):2794. [PubMed:35181693]


experimental experimental literature