Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mqsRA (relBE)/Xre-YgiU |
| Location | 3165873..3166566 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | mqsR | Uniprot ID | Q46865 |
| Locus tag | b3022 | Protein ID | NP_417494.1 |
| Coordinates | 3168248..3168544 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | mqsA | Uniprot ID | Q46864 |
| Locus tag | b3021 | Protein ID | NP_417493.1 |
| Coordinates | 3167851..3168246 (-) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b3017 | 3161257..3162669 | - | 1413 | NP_417489.1 | cell division protein FtsP | - |
| b3018 | 3162744..3163481 | - | 738 | NP_417490.1 | 1-acylglycerol-3-phosphate O-acyltransferase PlsC | - |
| b3019 | 3163715..3165973 | - | 2259 | NP_417491.1 | DNA topoisomerase IV subunit A | - |
| b3020 | 3166111..3167718 | - | 1608 | NP_417492.1 | putative deoxycholate binding periplasmic protein | - |
| b3021 | 3167851..3168246 | - | 396 | NP_417493.1 | antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA | Antitoxin |
| b3022 | 3168248..3168544 | - | 297 | NP_417494.1 | mRNA interferase toxin MqsR | Toxin |
| b3023 | 3168749..3169231 | - | 483 | YP_026197.1 | DNA-binding transcriptional repressor YgiV | - |
| b3024 | 3169284..3169676 | - | 393 | NP_417496.1 | BOF family protein YgiW | - |
| b3025 | 3169828..3170487 | + | 660 | NP_417497.1 | DNA-binding transcriptional activator QseB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11231.96 Da Isoelectric Point: 8.9070
>T1045 NP_417494.1 NC_000913:c3168544-3168248 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK
Download Length: 297 bp
>T1045 NC_000913:c3168544-3168248 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 132 a.a. Molecular weight: 14703.10 Da Isoelectric Point: 9.2136
>AT1045 NP_417493.1 NC_000913:c3168246-3167851 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR
Download Length: 396 bp
>AT1045 NC_000913:c3168246-3167851 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6100 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| T162 | Xylella fastidiosa 9a5c |
60.606 |
100 |
0.612 |
| T314 | Pseudomonas putida KT2440 |
57.732 |
98.98 |
0.571 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6100 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| AT314 | Pseudomonas putida KT2440 |
45.038 |
100 |
0.45 |
| AT162 | Xylella fastidiosa 9a5c |
41.985 |
100 |
0.42 |
Multiple sequence alignment
References
(1) Younghoon Kim et al. (2010) Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD. Environmental Microbiology 12(5):1105-21. [PubMed:20105222]
(2) Mikkel Christensen-Dalsgaard et al. (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Molecular Microbiology 75(2):333-48. [PubMed:19943910]
(3) Nathan Fraikin et al. (2019) Reassessing the Role of the Type II MqsRA Toxin-Antitoxin System in Stress Response and Biofilm Formation: mqsA Is Transcriptionally Uncoupled from mqsR. MBio 10(6):e02678-19. [PubMed:31848281]
(4) Breann L Brown et al. (2009) Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties. PLoS Pathogens 5(12):e1000706. [PubMed:20041169]