Detailed information of TA system TA000611

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): /Xre-COG2856
• Location: 1571120..1571917
• Replicon: chromosome
• Accession: NC_003923
• Organism: Staphylococcus aureus subsp. aureus MW2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MW1437
• Protein ID: 21283166
• Coordinates: 1571456..1571917 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MW1436
• Protein ID: 21283165
• Coordinates: 1571120..1571443 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW_RS07640 (MW1426)	1566856..1567413	-	558	WP_000645048.1	DUF2815 family protein	-
MW_RS07645 (MW1427)	1567439..1568605	-	1167	WP_000762552.1	DUF2800 domain-containing protein	-
MW_RS07650 (MW1428)	1568602..1568964	-	363	WP_000985979.1	hypothetical protein	-
MW_RS07655 (MW1429)	1568979..1569302	-	324	WP_000174994.1	hypothetical protein	-
MW_RS07660 (MW1430)	1569381..1569542	-	162	WP_001285963.1	DUF1270 family protein	-
MW_RS07665 (MW1431)	1569555..1569818	-	264	WP_001124190.1	helix-turn-helix domain-containing protein	-
MW_RS07670 (MW1432)	1569843..1570058	-	216	WP_001036302.1	hypothetical protein	-
MW_RS07675 (MW1433)	1570113..1570478	+	366	WP_001128433.1	hypothetical protein	-
MW_RS07680 (MW1434)	1570447..1570692	-	246	WP_001025401.1	DUF2829 domain-containing protein	-
MW_RS07685 (MW1435)	1570708..1570956	-	249	WP_000272860.1	helix-turn-helix domain-containing protein	-
MW_RS07690 (MW1436)	1571120..1571443	+	324	WP_001260485.1	helix-turn-helix domain-containing protein	Antitoxin
MW_RS07695 (MW1437)	1571456..1571917	+	462	WP_000525002.1	hypothetical protein	Toxin
MW_RS07700 (MW1438)	1571935..1572369	+	435	WP_000755718.1	hypothetical protein	-
MW_RS07705 (MW1439)	1572398..1572793	+	396	WP_000449653.1	hypothetical protein	-
MW_RS07710 (MW1441)	1572992..1573615	-	624	WP_001166482.1	hypothetical protein	-
MW_RS07715 (MW1442)	1573725..1574930	+	1206	WP_000264191.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP019574 Staphylococcus aureus strain USA400-0051 Prophage TA072683, TA072684, TA072687 1523368..1582098 0.003 View NZ_CP026073 Staphylococcus aureus strain FDAARGOS_42 Prophage TA093145, TA093146, TA093149 545174..593712 0.007	inside	Prophage	-	lukF-PV / lukS-PV	1526005..1592556	66551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 17943.33 Da
• Isoelectric Point: 5.0698

>T611 21283166 NC_003923:1571456-1571917 [Staphylococcus aureus subsp. aureus MW2]
MGLYEETLIQHAYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLHEIVEAHNYGVRNLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T611 NC_003923:1571456-1571917 [Staphylococcus aureus subsp. aureus MW2]
ATGGGATTATATGAAGAAACTTTAATACAACATGCTTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCTGTACCGCTACATGAAATTGTAGAAGCTCACAACTACGGTGTTCGTAATTTGTACGAGTT
GTCAGAGTATCTGCAGTTGAGTGAATCATACATACTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACGTTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12439.16 Da
• Isoelectric Point: 9.3811

>AT611 21283165 NC_003923:1571120-1571443 [Staphylococcus aureus subsp. aureus MW2]
MRTSAEIGKLIKQLRKENNVNLTDFAIKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQPENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 324 bp

>AT611 NC_003923:1571120-1571443 [Staphylococcus aureus subsp. aureus MW2]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAAAATAATGTGAATTTAACTGATTTTGCAAT
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATACGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAACCAGAAAACGAAGTACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCTGATTATATAAGAAGCAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T4244	Staphylococcus aureus subsp. aureus str. Newman	94.771	100	0.948

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT4244	Staphylococcus aureus subsp. aureus str. Newman	53.922	98.077	0.529

Multiple sequence alignment

References

(1) Gul Habib et al. (2018) Bioinformatics and Functional Assessment of Toxin-Antitoxin Systems in Staphylococcus aureus. Toxins 10(11):473. [PubMed:30441856]


 experimental literature