Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) /Xre-COG2856
Location 323285..324283 Replicon chromosome
Accession NC_009641
Organism Staphylococcus aureus subsp. aureus str. Newman

Toxin (Protein)


Gene name - Uniprot ID A0A380E6V7
Locus tag NWMN_RS01465 Protein ID WP_000525007.1
Coordinates 323285..323749 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag NWMN_RS01470 Protein ID WP_000333630.1
Coordinates 323762..324076 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWMN_RS01445 (NWMN_0262) 318821..320752 + 1932 Protein_279 YSIRK domain-containing triacylglycerol lipase Lip2/Geh -
NWMN_RS01450 (NWMN_0263) 320846..322051 - 1206 WP_000264186.1 site-specific integrase -
NWMN_RS01455 322162..322341 + 180 WP_000337827.1 hypothetical protein -
NWMN_RS01460 (NWMN_0264) 322321..323253 - 933 WP_000392183.1 hypothetical protein -
NWMN_RS01465 (NWMN_0265) 323285..323749 - 465 WP_000525007.1 hypothetical protein Toxin
NWMN_RS01470 323762..324076 - 315 WP_000333630.1 helix-turn-helix domain-containing protein Antitoxin
NWMN_RS01475 324228..324464 + 237 WP_001121116.1 helix-turn-helix domain-containing protein -
NWMN_RS01480 324478..324657 + 180 WP_000438352.1 hypothetical protein -
NWMN_RS01485 (NWMN_0267) 324758..325240 - 483 WP_000394410.1 hypothetical protein -
NWMN_RS01490 (NWMN_0268) 325300..326049 + 750 WP_001148586.1 phage antirepressor KilAC domain-containing protein -
NWMN_RS01495 326065..326283 + 219 WP_000993180.1 hypothetical protein -
NWMN_RS01500 (NWMN_0269) 326313..326576 + 264 WP_001124198.1 helix-turn-helix domain-containing protein -
NWMN_RS01505 326588..326749 + 162 WP_000066021.1 DUF1270 family protein -
NWMN_RS01510 (NWMN_0270) 326841..327101 + 261 WP_000291089.1 DUF1108 family protein -
NWMN_RS01515 (NWMN_2615) 327111..327332 + 222 WP_000815401.1 DUF2483 family protein -
NWMN_RS01520 (NWMN_0271) 327325..328113 + 789 WP_000134097.1 ATP-binding protein -
NWMN_RS01525 (NWMN_0272) 328142..328693 + 552 WP_000704705.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320846..363190 42344


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(4-62)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 18100.42 Da        Isoelectric Point: 4.7904

>T4244 WP_000525007.1 NC_009641:c323749-323285 [Staphylococcus aureus subsp. aureus str. Newman]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSNFNNRKFENYAR
RHGFISAVPLREIVEAHNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEFSITFEPLRVFKLHHID

Download         Length: 465 bp

>T4244 NC_009641:c323749-323285 [Staphylococcus aureus subsp. aureus str. Newman]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCGCACAATTACGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGTACTC
ACTACGGCGAATTCTCAATTACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCATATTGATTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT4244 WP_000333630.1 NC_009641:c324076-323762 [Staphylococcus aureus subsp. aureus str. Newman]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT4244 NC_009641:c324076-323762 [Staphylococcus aureus subsp. aureus str. Newman]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATTTA
GAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T611 Staphylococcus aureus subsp. aureus MW2

94.771

100

0.948

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT611 Staphylococcus aureus subsp. aureus MW2

53.922

98.077

0.529

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A380E6V7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References

(1) Nour Sayed et al. (2012) Functional and structural insights of a Staphylococcus aureus apoptotic-like membrane peptide from a toxin-antitoxin module. The Journal of Biological Chemistry 287(52):43454-63. [PubMed:23129767] experimental


experimental experimental literature