Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00791  |
| T6SS type | Type i4b |
| Strain | Edwardsiella tarda PPD130/91 |
| Replicon (GenBank) | chromosome (AY424360) |
| Location | 3625..24399 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Virulence in blue gourami fish; virulence in several other fish models; invasion of cultured carp epithelial cells. In the evpB and evpC mutants, secreted protein EvpC disappeared from their proteomes, whereas the evpA mutant lacked both EvpA and EvpC. |
| References | [1] Zheng J et al (2007) Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 66(5):1192-206. [PudMed:17986187] [2] Chakraborty S et al (2011) Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 286(45):39417-30. [PudMed:21953460] [3] Hu W et al (2014) A Disordered Region in the EvpP Protein from the Type VI Secretion System of Edwardsiella tarda is Essential for EvpC Binding. PLoS One. 9(11):e110810. [PudMed:25401506] [4] Rao PS et al (2004) Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 53(2):573-86. [PudMed:15228535] [5] Jobichen C et al (2010) Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 5(9):e12910. [PudMed:20886112] [6] Chakraborty S et al (2010) Temperature and Mg2+ sensing by a novel PhoP-PhoQ two-component system for regulation of virulence in Edwardsiella tarda. J Biol Chem. 285(50):38876-88. [PudMed:20937832] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | PAAR | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| Locus_01 | 90..788 | - | 699 | 158512111 | putative transporting ATPase | ||
| Locus_02 | 739..1581 | - | 843 | 158512112 | hypothetical protein | ||
| Locus_03 | 1654..1821 | - | 168 | 158512113 | hypothetical protein | ||
| Locus_04 | 1843..3297 | - | 1455 | 158512114 | hypothetical protein | ||
| Locus_05 (evpP) | 3625..4182 | + | 558 | 158512115 | EvpP | ||
| T6CP010874 | TssB | Locus_06 (evpA) | 4431..4946 | + | 516 | 40287636 | EvpA |
| T6CP010875 | TssC | Locus_07 (evpB) | 4943..6430 | + | 1488 | 40287637 | EvpB |
| T6CP010876 | TssD | Locus_08 (evpC) | 6500..6991 | + | 492 | 40287638 | EvpC |
| Locus_09 (evpD) | 7088..8311 | + | 1224 | 40287639 | EvpD | ||
| T6CP010877 | TssE | Locus_10 (evpE) | 8320..8796 | + | 477 | 45332447 | EvpE |
| T6CP010878 | TssF | Locus_11 (evpF) | 8800..10641 | + | 1842 | 158512125 | EvpF |
| T6CP010879 | TssG | Locus_12 (evpG) | 10638..11663 | + | 1026 | 158512126 | EvpG |
| T6CP010880 | TssH | Locus_13 (evpH) | 11782..14394 | + | 2613 | 158512127 | EvpH |
| T6CP010881 | TssI | Locus_14 (evpI) | 14394..16379 | + | 1986 | 158512116 | EvpI |
| T6CP012276 | PAAR | Locus_15 (evpJ) | 16475..16777 | + | 303 | 158512117 | EvpJ |
| T6CP010882 | TssA | Locus_16 (evpK) | 16791..17858 | + | 1068 | 158512118 | EvpK |
| T6CP010883 | TssJ | Locus_17 (evpL) | 17779..18486 | + | 708 | 158512119 | EvpL |
| T6CP010884 | TssK | Locus_18 (evpM) | 18585..19973 | + | 1389 | 158512120 | EvpM |
| T6CP010885 | TssL | Locus_19 (evpN) | 19970..20620 | + | 651 | 158512121 | EvpN |
| T6CP010886 | TssM | Locus_20 (evpO) | 20608..24399 | + | 3792 | 158512122 | EvpO |
| Locus_21 | 24531..25418 | - | 888 | 158512123 | hypothetical protein | ||
| Locus_22 | 25375..26460 | - | 1086 | 158512124 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0318 |
Y113_RS04835 (esrC) | protein | TCS-HK | Edwardsiella tarda strain PPD130/91 | chromosome (NZ_JACQ01000008) | 20632..23367 (-) | 1624663318 | EvpQ protein level (↓) |
| REG0319 |
Y113_RS04830 (esrB) | protein | TCS-RR | Edwardsiella tarda strain PPD130/91 | chromosome (NZ_JACQ01000008) | 20006..20635 (+) | 1940020585 | EvpA and EvpC protein concentration (↓) |
| REG0364 |
Y113_RS11250 (RpoS) | protein | Transcription regulator | Edwardsiella tarda PPD130/91 | chromosome (NZ_JACQ01000026) | 9122..10108 (-) | 502612732 | EvpQ transcription and protein level (↑) |
| REG0365 |
Y113_RS02460 (Fur) | protein | Global regulator | Edwardsiella tarda PPD130/91 | chromosome (NZ_JACQ01000003) | 461902..462351 (+) | 735678383 | EvpQ protein level (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01887 |
Y113_RS08110 (EvpQ) | Tse | - | Edwardsiella tarda PPD130/91 | chromosome (NZ_JACQ01000015) | 41196..41717 (-) | 502611890 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|