Detailed information of regulator
Summary
| Regulator ID | REG0365  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00791 (Type i4b) |
| Strain | Edwardsiella tarda PPD130/91 |
| Replicon | chromosome |
| Sequence | Protein sequence (150 a.a.); Nucleotide sequence (450 bp) |
| Description | When culturing in DMEM, evpQ is negatively regulated by Fur and positively regulated by EsrC. |
| Reference |
External database links
| Locus tag (Gene) | Y113_RS02460 (Fur) |
| Coordinates (Strand) | 461902..462351 (+) |
| NCBI ID | 735678383 |
| RefSeq | NZ_JACQ01000003 |
| Uniprot ID | A2TEZ4_EDWTA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| EvpQ protein level | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FUR | PF01475.21 | 7.7e-49 | 10..130 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-149 | MTDNNTALKKAGLKVTLPRLKILEVLQDPTCHHVSAEDLYKKLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDESIEKRQREIAKKHGIQLTNHSLYLYGHCAEGDCRSDEALHDEKA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9967 | - | - |
Protein sequence of regulator: 150 a.a. .
>REG0365 NZ_JACQ01000003:461902-462351 [Edwardsiella tarda PPD130/91]
MTDNNTALKKAGLKVTLPRLKILEVLQDPTCHHVSAEDLYKKLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDESIEKRQREIAKKHGIQLTNHSLYLYGHCAEGDCRSDEALHDEKA*
MTDNNTALKKAGLKVTLPRLKILEVLQDPTCHHVSAEDLYKKLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDESIEKRQREIAKKHGIQLTNHSLYLYGHCAEGDCRSDEALHDEKA*
Nucleotide sequence of regulator: 450 bp .
>REG0365 NZ_JACQ01000003:461902-462351 [Edwardsiella tarda PPD130/91]
ATGACTGACAACAACACCGCGTTAAAGAAAGCTGGCCTGAAAGTCACGCTTCCCCGTCTCAAGATTCTGGAAGTACTGCA
AGATCCTACGTGCCATCACGTCAGTGCTGAAGACTTGTACAAGAAGCTGATCGACATGGGCGAGGAGATCGGTCTGGCGA
CCGTATACCGCGTGCTCAACCAGTTTGACGATGCCGGCATCGTTACCCGCCATAACTTTGAGGGCGGTAAATCGGTCTTT
GAGCTGACCCAACAGCACCACCACGATCACCTGATCTGCCTGGACTGCGGCAAGGTCATCGAGTTCAGCGATGAGTCCAT
CGAGAAGCGTCAGCGTGAAATCGCCAAAAAACACGGCATCCAGCTGACCAACCACAGCCTGTATCTCTACGGCCACTGCG
CCGAGGGCGACTGCCGCAGCGATGAGGCCCTGCACGACGAAAAGGCCTAA
ATGACTGACAACAACACCGCGTTAAAGAAAGCTGGCCTGAAAGTCACGCTTCCCCGTCTCAAGATTCTGGAAGTACTGCA
AGATCCTACGTGCCATCACGTCAGTGCTGAAGACTTGTACAAGAAGCTGATCGACATGGGCGAGGAGATCGGTCTGGCGA
CCGTATACCGCGTGCTCAACCAGTTTGACGATGCCGGCATCGTTACCCGCCATAACTTTGAGGGCGGTAAATCGGTCTTT
GAGCTGACCCAACAGCACCACCACGATCACCTGATCTGCCTGGACTGCGGCAAGGTCATCGAGTTCAGCGATGAGTCCAT
CGAGAAGCGTCAGCGTGAAATCGCCAAAAAACACGGCATCCAGCTGACCAACCACAGCCTGTATCTCTACGGCCACTGCG
CCGAGGGCGACTGCCGCAGCGATGAGGCCCTGCACGACGAAAAGGCCTAA