SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0318
Regulator type	protein
Regulatory pathway (from literatures)	TCS-HK
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020)
Related T6SS	T6SS00791 (Type i4b)
Strain	Edwardsiella tarda strain PPD130/91
Replicon	chromosome
Sequence	Protein sequence (912 a.a.); Nucleotide sequence (2736 bp)
Description	The LacZ reporter study suggests that EsrC regulated the transcription of the evpA-H transcriptional unit; only one promoter sequence was found, and the regulation of EsrC on evpA is possibly direct. EvpA and EvpC were shown to be regulated by EsrC earlier in the 2-DE studies; When culturing in DMEM, evpQ is negatively regulated by Fur and positively regulated by EsrC.
Reference	[1] Zheng J, Tung SL, Leung KY. Regulation of a type III and a putative secretion system in Edwardsiella tarda by EsrC is under the control of a two-component system, EsrA-EsrB. Infect Immun. 2005 Jul;73(7):4127-37. PMID: 15972502 [2] Li DY, Liu YL, Liao XJ, et al. Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element in Edwardsiella piscicida. Front Microbiol. 2021 Mar 11;12:643498. doi: 10.3389/fmicb.2021.643498. eCollection 2021. PMID: 33776977

External database links

Locus tag (Gene)	Y113_RS04835 (esrC)
Coordinates (Strand)	20632..23367 (-)
NCBI ID	1624663318
RefSeq	NZ_JACQ01000008
Uniprot ID	-
KEGG ID	-
KEGG pathway (determined by BlastKOALA)	map02020
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
EvpQ protein level	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
HATPase_c	PF02518.28	2e-20	501..605
Response_reg	PF00072.26	9.6e-20	684..798
HisKA	PF00512.27	2.1e-17	389..453
Hpt	PF01627.25	1.8e-10	829..904

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-14	MSRHIWRTSLSFRL
Transmembrane helix	15-37	ALILSGFIALFWLLSTGVTLLLN
Inside	38-285	FEQTKSSLQRRLTATVAETVYELSSELLDVSRDVDTLMRCWHRLDDGVASGAYYLTARYIHDMTAPPDPAARISRAKAFVEAYGSGGLGNLVDTFVLLDGGVVLSSAGGQPTDTPLHIEQIRALRDQAVGNHIIWSKPYRSVGGNWRVIAAKRDLLTGALVGMTVNLHPSFSAVEKTPDGETIVWLDREGQPIIPLSAALSQSLPTCQREMESNFDEVFSICREVAPVGWKLLLFTPSRQITDAAFAA
Transmembrane helix	286-308	LHRYLPVALLLLVALVGLLYLVL
Outside	309-911	QRTLGRTLAGIMQRLTPNVAVADLPPLVVAGEDELGRIAQVYNRLLSAVKAQYAQLEARVAERTVELERARRQAERASANKSEHLNSISHEIRTPLNGIIGALTLLENGENTPEQRDLLDTGLKCSRHLLEIINNLLDFSRIESGQMVVNPEYLDPLPLIDQAMLTVQVPALEKGLTLHCLLAPSFPQTLYTDGLRLRQILINLLGNAVKFTPHGEVVLHGWSEQDRLCFRVQDTGPGIDDARINDIFTVFHQVDCHIAGSGLGLPIARSLSRLLGGELSCEKSAIGARFRLALPLEANGSVPIAPPEDKGSIVAPLSLHPQLSAWGYHPLAGDNPDLDAPELSYLPGRLRQHLDGETQTTGAREQMPVSPWSLQVLVVDDVDTNQDIVGRMLRQQGHKVYTASSGSEALVLGRGHVFDLVLMDMRMPGLSGDETLHLWRDSVNGMLDPDCPIIALTANAQPGERKRLQDAGFNEYLTKPVTPAMLSRALEYTADIQLTRGIELSPNRAGEIPLLGQDPALRRRLQEDVLHYLAQLTQAVAQQDAPAVRQALHTLKGLAGQGGLTLVHEAAARWEQALEGGMPMPDKALESLSRLVHSEFEAD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9933	-	-

Protein sequence of regulator: 912 a.a. .

                                    >REG0318 NZ_JACQ01000008:c23367-20632 [Edwardsiella tarda PPD130/91]
MSRHIWRTSLSFRLALILSGFIALFWLLSTGVTLLLNFEQTKSSLQRRLTATVAETVYELSSELLDVSRDVDTLMRCWHR
LDDGVASGAYYLTARYIHDMTAPPDPAARISRAKAFVEAYGSGGLGNLVDTFVLLDGGVVLSSAGGQPTDTPLHIEQIRA
LRDQAVGNHIIWSKPYRSVGGNWRVIAAKRDLLTGALVGMTVNLHPSFSAVEKTPDGETIVWLDREGQPIIPLSAALSQS
LPTCQREMESNFDEVFSICREVAPVGWKLLLFTPSRQITDAAFAALHRYLPVALLLLVALVGLLYLVLQRTLGRTLAGIM
QRLTPNVAVADLPPLVVAGEDELGRIAQVYNRLLSAVKAQYAQLEARVAERTVELERARRQAERASANKSEHLNSISHEI
RTPLNGIIGALTLLENGENTPEQRDLLDTGLKCSRHLLEIINNLLDFSRIESGQMVVNPEYLDPLPLIDQAMLTVQVPAL
EKGLTLHCLLAPSFPQTLYTDGLRLRQILINLLGNAVKFTPHGEVVLHGWSEQDRLCFRVQDTGPGIDDARINDIFTVFH
QVDCHIAGSGLGLPIARSLSRLLGGELSCEKSAIGARFRLALPLEANGSVPIAPPEDKGSIVAPLSLHPQLSAWGYHPLA
GDNPDLDAPELSYLPGRLRQHLDGETQTTGAREQMPVSPWSLQVLVVDDVDTNQDIVGRMLRQQGHKVYTASSGSEALVL
GRGHVFDLVLMDMRMPGLSGDETLHLWRDSVNGMLDPDCPIIALTANAQPGERKRLQDAGFNEYLTKPVTPAMLSRALEY
TADIQLTRGIELSPNRAGEIPLLGQDPALRRRLQEDVLHYLAQLTQAVAQQDAPAVRQALHTLKGLAGQGGLTLVHEAAA
RWEQALEGGMPMPDKALESLSRLVHSEFEAD*

                                 

Nucleotide sequence of regulator: 2736 bp .

                                    >REG0318 NZ_JACQ01000008:c23367-20632 [Edwardsiella tarda PPD130/91]
ATGTCCCGTCATATCTGGCGCACCTCCCTCTCGTTCCGCCTGGCGCTGATCCTCAGTGGATTTATTGCCCTGTTCTGGCT
GCTGAGTACCGGCGTGACGCTGCTGCTGAACTTTGAGCAGACCAAGTCATCGCTGCAGCGCCGTTTGACGGCGACCGTGG
CCGAAACGGTCTATGAGCTGAGCTCTGAGCTGCTGGATGTCAGCCGTGACGTGGATACGCTGATGCGCTGTTGGCACCGC
CTGGACGACGGCGTCGCCTCTGGCGCCTACTACCTGACGGCGCGCTACATTCACGATATGACCGCGCCGCCGGATCCGGC
GGCGCGCATCAGCCGCGCGAAGGCCTTTGTCGAAGCCTACGGCAGCGGCGGCTTGGGCAATCTGGTGGATACCTTCGTCC
TGCTGGACGGCGGCGTGGTGCTGTCCAGCGCCGGCGGTCAGCCGACCGACACCCCGCTGCACATTGAACAGATCCGGGCG
CTGCGCGATCAGGCCGTCGGCAATCACATCATCTGGAGTAAGCCTTATCGCAGCGTCGGGGGAAACTGGCGAGTGATTGC
GGCGAAGCGGGATTTGCTCACCGGTGCGCTGGTCGGCATGACGGTCAACCTGCATCCCTCCTTTAGCGCCGTAGAGAAAA
CCCCAGATGGTGAAACGATCGTTTGGCTCGATCGGGAGGGGCAGCCGATCATTCCGCTGTCCGCCGCGCTGAGTCAGAGC
CTGCCGACGTGCCAGCGCGAAATGGAGAGTAATTTTGACGAGGTATTCTCCATCTGCCGCGAGGTCGCCCCGGTCGGCTG
GAAGCTGCTGCTGTTCACACCGAGCCGCCAGATTACGGACGCGGCGTTCGCCGCATTGCACCGCTATCTGCCGGTGGCGC
TGCTGCTGCTGGTCGCGCTGGTGGGGCTCTTGTACCTGGTGCTGCAGCGCACGCTGGGGCGTACGCTGGCCGGCATTATG
CAGCGCCTGACGCCCAACGTGGCGGTGGCCGATCTGCCGCCCCTGGTGGTGGCGGGAGAGGATGAGCTGGGGCGCATTGC
GCAGGTCTATAATCGCCTGCTGTCGGCGGTCAAGGCGCAGTATGCGCAGCTGGAGGCGCGGGTCGCCGAACGAACGGTAG
AGCTGGAGCGGGCGCGGCGCCAGGCCGAGCGCGCCAGCGCCAACAAGAGCGAGCACCTGAACAGCATCAGCCATGAAATT
CGCACCCCGTTGAATGGGATTATCGGTGCGCTGACGCTGCTGGAGAACGGTGAGAATACGCCGGAGCAGCGTGATTTGCT
GGATACCGGGCTTAAGTGTTCGCGCCATCTGCTGGAGATCATCAATAACCTGCTGGACTTCTCGCGCATTGAGTCGGGAC
AGATGGTGGTGAACCCGGAGTACCTCGATCCGCTGCCGCTGATCGATCAGGCGATGCTGACGGTGCAGGTGCCGGCGCTG
GAGAAGGGATTGACCCTGCACTGCCTGCTGGCGCCGTCGTTTCCGCAGACGCTGTATACCGACGGGCTGCGCCTGCGCCA
GATCTTGATCAACCTGCTGGGCAACGCGGTCAAGTTTACCCCCCATGGCGAGGTGGTGCTACACGGCTGGAGCGAGCAGG
ATCGACTCTGTTTCCGGGTGCAGGATACCGGCCCGGGCATCGATGACGCGCGGATCAACGATATTTTTACCGTATTTCAT
CAGGTCGATTGCCACATCGCCGGCAGCGGCCTGGGGCTGCCGATCGCCCGCAGCCTGTCGCGCCTGCTGGGGGGTGAGCT
CTCCTGCGAGAAAAGCGCGATCGGCGCCCGTTTCCGCCTGGCGCTGCCGCTGGAGGCGAACGGGAGTGTCCCCATCGCGC
CGCCGGAGGATAAGGGCAGCATTGTCGCGCCGCTCAGCCTGCACCCACAGCTGTCGGCCTGGGGATACCACCCCCTGGCG
GGCGACAATCCCGATCTGGACGCGCCGGAGCTGAGCTACCTGCCCGGCCGCCTGCGGCAGCATTTGGATGGCGAAACGCA
GACGACCGGCGCGCGGGAGCAGATGCCGGTATCGCCCTGGTCGCTGCAGGTACTGGTGGTGGACGATGTCGATACCAATC
AGGATATCGTCGGGCGCATGCTGCGCCAGCAGGGGCACAAAGTGTATACCGCCTCCAGCGGCTCTGAAGCGCTGGTGCTG
GGGCGCGGGCACGTGTTTGACTTAGTCCTGATGGATATGCGTATGCCGGGGCTCTCCGGCGACGAAACGCTGCATCTGTG
GCGGGATAGCGTGAATGGAATGCTCGACCCCGACTGCCCGATCATCGCGCTGACGGCCAATGCGCAGCCCGGCGAACGCA
AGCGGCTGCAGGACGCTGGTTTTAATGAGTATCTCACCAAGCCGGTGACCCCCGCGATGCTCTCCAGAGCCCTGGAGTAC
ACGGCCGATATCCAGTTAACCCGGGGGATCGAGCTGTCGCCTAACCGCGCGGGCGAGATACCGCTGCTCGGACAGGATCC
CGCGCTGCGGCGGCGTCTGCAGGAGGATGTCTTGCACTATCTGGCGCAGCTGACGCAGGCGGTCGCGCAGCAGGATGCCC
CCGCGGTGCGGCAGGCGCTGCATACGCTAAAAGGGCTGGCCGGGCAGGGCGGTCTGACGCTGGTGCATGAGGCGGCGGCG
CGCTGGGAGCAGGCGCTGGAGGGCGGGATGCCGATGCCAGACAAGGCGCTGGAAAGCCTATCCCGCCTGGTGCACAGTGA
ATTCGAGGCGGATTAA

                                