Detailed information of regulator
Summary
| Regulator ID | REG0319  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-RR |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020) |
| Related T6SS | T6SS00791 (Type i4b) |
| Strain | Edwardsiella tarda strain PPD130/91 |
| Replicon | chromosome |
| Sequence | Protein sequence (210 a.a.); Nucleotide sequence (630 bp) |
| Description | Use of proteomics to identify disruption of esrB affected the expression of EvpA and EvpC |
| Reference |
External database links
| Locus tag (Gene) | Y113_RS04830 (esrB) |
| Coordinates (Strand) | 20006..20635 (+) |
| NCBI ID | 1940020585 |
| RefSeq | NZ_JACQ01000008 |
| Uniprot ID | - |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map02020 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| EvpA and EvpC protein concentration | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 3.1e-27 | 5..116 |
| GerE | PF00196.21 | 7.5e-19 | 146..200 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-209 | MPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWSDMRIVVVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQILKLVAEGKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9934 | - | - |
Protein sequence of regulator: 210 a.a. .
>REG0319 NZ_JACQ01000008:20006-20635 [Edwardsiella tarda PPD130/91]
MPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWSDMRIV
VVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQILKLVAE
GKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF*
MPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWSDMRIV
VVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQILKLVAE
GKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF*
Nucleotide sequence of regulator: 630 bp .
>REG0319 NZ_JACQ01000008:20006-20635 [Edwardsiella tarda PPD130/91]
TTGCCTCTGAACATTCTGATCGTTGAAGATCATGCCTTGCTAGCCGATGGCATCCGTAATCTCTTGGACAGCCAGCCCCG
CTACCGGGTCGTGGGCGCCGTCTCGAATGGGCTGGCCGTTTATGAGGCCTGCCAGCGCCTCTCGCCCGATCTGGTCTTAC
TCGATCTGGGTCTGCCGGGCATGGACGGCGTTGACGTGATCCGTCAGCTGCGGCGTCGCTGGAGCGATATGCGTATCGTT
GTCGTCACCGCCTCCTCTGAGGCGCGGCGCGGCAGCGAAGCCCTCGCCGCCGGCGCGCTCGCCTACGTCCTGAAGCAAAG
CCCGCAGCAAACCCTGCTGGCGGCCATGCAGCATGCCTCGCTGGGGAAAACCTTTGTCGATCCCGCCCTCCGGCTGGACT
CCCTGGTAGCCTCGTCCGATATGGCTCAGGAGCCGCCGCTGACCCTGCGCGAACGGCAAATTCTCAAGCTGGTCGCCGAG
GGCAAGCGCAACCGGGATATCGCGGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCACCGGCTCAACCTGATGCG
CAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAACTGGGCCCGCCGCCTGGGGGTTCTGGAGTTTTAA
TTGCCTCTGAACATTCTGATCGTTGAAGATCATGCCTTGCTAGCCGATGGCATCCGTAATCTCTTGGACAGCCAGCCCCG
CTACCGGGTCGTGGGCGCCGTCTCGAATGGGCTGGCCGTTTATGAGGCCTGCCAGCGCCTCTCGCCCGATCTGGTCTTAC
TCGATCTGGGTCTGCCGGGCATGGACGGCGTTGACGTGATCCGTCAGCTGCGGCGTCGCTGGAGCGATATGCGTATCGTT
GTCGTCACCGCCTCCTCTGAGGCGCGGCGCGGCAGCGAAGCCCTCGCCGCCGGCGCGCTCGCCTACGTCCTGAAGCAAAG
CCCGCAGCAAACCCTGCTGGCGGCCATGCAGCATGCCTCGCTGGGGAAAACCTTTGTCGATCCCGCCCTCCGGCTGGACT
CCCTGGTAGCCTCGTCCGATATGGCTCAGGAGCCGCCGCTGACCCTGCGCGAACGGCAAATTCTCAAGCTGGTCGCCGAG
GGCAAGCGCAACCGGGATATCGCGGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCACCGGCTCAACCTGATGCG
CAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAACTGGGCCCGCCGCCTGGGGGTTCTGGAGTTTTAA