Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00466 experimental
T6SS type Type ii
Strain Francisella tularensis subsp. holarctica LVS
Replicon (RefSeq) chromosome (NC_007880)
Location 102157..124300
Function anti-eukaryotic; effector translocation; virulence
Comment Growth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model. IglE of F. novicida is required for T6SS in vitro.
References
[1] Nano FE et al (2007) The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
[2] Broms JE et al (2012) Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]
[3] Lindgren M et al (2013) Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623]
[4] Broms JE et al (2011) IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239]
[5] Lindgren M et al (2013) The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941]
[6] de Bruin OM et al (2011) The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]
[7] Broms JE et al (2012) DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651]
[8] Brms JE, Meyer L, Sjstedt A. et al (2017) A mutagenesis-based approach identifies amino acids in the N-terminal part of Francisella tularensis IglE that critically control Type VI system-mediated secretion.. Virulence. 8(6):821-847. [PudMed:27830989]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentIglCIglDIglEIglFIglGIglHIglIIglJPdpAPdpBPdpCPdpDPdpETssBTssCTssITssL
Number11111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
FTL_RS00505 98287..98684 - 398 pseudogene DUF3568 domain-containing protein
FTL_RS00510 98687..99097 - 411 WP_003014086.1 DUF3568 domain-containing protein
FTL_RS00515 99118..99510 - 393 WP_003014087.1 DUF3568 domain-containing protein
FTL_RS00520 99799..100251 + 453 pseudogene nicotinamide mononucleotide transporter
FTL_RS10125 100311..101191 + 881 WP_011457326.1 IS630 family transposase
FTL_RS00535 101192..102064 - 873 WP_011457343.1 MFS transporter
FTL_RS10795 102049..102771 + 723 pseudogene Pathogenicity determinant protein D
T6CP004815 TssB FTL_RS00550 (tssB) 102852..103406 + 555 WP_003016161.1 type VI secretion system contractile sheath small subunit
T6CP004816 TssC FTL_RS00555 (tssC) 103420..104940 + 1521 WP_010031646.1 type VI secretion system contractile sheath large subunit
T6CP004817 IglC FTL_RS00560 104957..105586 + 630 WP_003016165.1 hypothetical protein
T6CP004818 IglD FTL_RS00565 105607..106803 + 1197 WP_011457345.1 hypothetical protein
T6CP004819 PdpE FTL_RS00570 106810..107385 - 576 WP_003016169.1 hypothetical protein
T6CP004820 PdpC FTL_RS00575 (pdpC) 107403..111389 - 3987 WP_003016172.1 pathogenicity determinant protein PdpC
T6CP004821 IglJ FTL_RS00580 111429..112202 - 774 WP_003016175.1 hypothetical protein
T6CP004822 IglI FTL_RS00585 112168..113319 - 1152 WP_003016179.1 hypothetical protein
T6CP004823 TssL FTL_RS00590 113341..113964 - 624 WP_003016181.1 DotU family type IV/VI secretion system protein
T6CP004824 IglH FTL_RS00595 113977..115407 - 1431 WP_003016182.1 hypothetical protein
T6CP004825 IglG FTL_RS00600 115420..115941 - 522 WP_003016185.1 DUF4280 domain-containing protein
T6CP004826 IglF FTL_RS00605 116009..117673 - 1665 WP_003016188.1 hypothetical protein
T6CP004827 TssI FTL_RS00610 117678..118172 - 495 WP_003016192.1 hypothetical protein
T6CP004828 IglE FTL_RS00615 118185..118562 - 378 WP_003018235.1 hypothetical protein
T6CP004829 PdpB FTL_RS00620 118552..121833 - 3282 WP_011457346.1 hypothetical protein
T6CP004830 PdpA FTL_RS00625 121838..124300 - 2463 WP_003016195.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00563 experimental FTT_1347 (VgrG) - - Francisella tularensis subsp. holarctica LVS chromosome (NC_007880) 117678..118172 (-) 89255560
EFF00562 experimental FTL_0118 (IglI) - - Francisella tularensis subsp. holarctica LVS chromosome (NC_007880) 112168..113319 (-) 89255555

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector